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Original Article

Telomerase activation, cellular immortalization and cancer

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Pages 123-129 | Published online: 08 Jul 2009

References

  • McClintock B. The stability of broken ends of chromosomes in Zea mays. Genetics 1941; 26: 234–82
  • Haber JE, Thorburn PC. Healing of broken linear dicentric chromosomes in yeast. Genetics 1984; 106: 207–26
  • Olovnikov AM. Principle of marginotomy in template synthesis of polynucleotides (in Russian). Dokl Akad Nauk SSSR 1971; 201: 1496–9
  • Olovnikov AM. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon. J Theor Biol 1973; 41: 181–90
  • Watson JD. Origin of concatemeric T7 DNA. Nature New Biol 1972; 239: 197–201
  • Meyerson M. Role of telomerase in normal and cancer cells. J Clin Oncol 2000; 18: 2626–34
  • Moyzis RK, Buckingham JM, Cram LS, Danl M, Deaven LL, Jones MD. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci USA 1988; 85: 6622–6
  • Meyne J, Ratliff RL, Moyzis RK. Conservation of the human telomere sequence (TTAGGG)n among vertebrates. Proc Natl Acad Sci USA 1989; 86: 7049–53
  • de Lange T, Shiue L, Myers RM, Cox DR, Naylor SR, Killery AM. Structure and variability of human chromosome ends. Mol Cell Biol 1990; 10: 518–27
  • Henderson ER, Blackburn EH. An overhanging 3′ terminus is a conserved feature of telomeres. Mol Cell Biol 1989; 9: 345–8
  • Makarov VL, Hirose Y, Langmore JP. Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell 1997; 88: 657–66
  • McElligott R, Wellinger RJ. The terminal DNA structure of mammalian chromosomes. Embo J 1997; 16: 3705–14
  • Wright WE, Tesmer VM, Huffman KE, Levene SD, Shay JW. Normal human chromosomes have long G-rich telomeric overhangs at one end. Genes Dev 1997; 11: 2801–9
  • Griffith JD, Comeau L, Rosenfield S, Stansel RM, Bianchi A, Moss H. Mammalian telomeres end in a large duplex loop. Cell 1999; 97: 503–14
  • Chong L, van Steensel B, Broccoli D, Erdjument-Bromage H, Hanish J, Tempst P. A human telomeric protein. Science 1995; 270: 1663–7
  • Broccoli D, Smogorzewska A, Chong L, de Lange T. Human telomeres contain two distinct Myb-related proteins, TRF1 and TRF2. Nat Genet 1997; 17: 231–5
  • Bilaud T, Brun C, Ancelin K, Koering CE, Laroche T, Gilson E. Telomeric localization of TRF2, a novel human telobox protein. Nat Genet 1997; 17: 236–9
  • van Steensel B, de Lange T. Control of telomere length by the human telomeric protein TRF1. Nature 1997; 385: 740–3
  • van Steensel B, Smogorzewska A, de Lange T. TRF2 protects human telomeres from end-to-end fusions. Cell 1998; 92: 401–13
  • Smith S, Giriat I, Schmitt A, de Lange T. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres. Science 1998; 282: 1484–7
  • Smith S, de Lange T. Tankyrase promotes telomere elongation in human cells. Curr Biol 2000; 10: 1299–302
  • Li B, Oestreich S, de Lange T. Identification of human Rapl: implications for telomere evolution. Cell 2000; 101: 471–83
  • Greider CW, Blackburn EH. A telomeric sequence in the RNA of Tetrahymena telomerase required for telomere repeat synthesis. Nature 1989; 337: 331–7
  • Yu GL, Bradley JD, Attardi LD, Blackburn EH. In vivo alteration of telomere sequences and senescence caused by mutated Tetrahymena telomerase RNAs. Nature 1990; 344: 126–32
  • Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP. The RNA component of human telomerase. Science 1995; 269: 1236–41
  • Singer MS, Gottschling DE. TLC1: template RNA component of Saccharomyces cerevisiae telomerase. Science 1994; 266: 404–9
  • Lingner J, Hughes TR, Shevchenko A, Mann M, Lundblad V, Cech TR. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 1997; 276: 561–7
  • Counter CM, Meyerson M, Eaton EN, Weinberg RA. The catalytic subunit of yeast telomerase. Proc Natl Acad Sci U S A 1997; 94: 9202–7
  • Nakamura TM, Morin GB, Chapman KB, Weinrich SL, Andrews WH, Lingner J. Telomerase catalytic subunit homologs from fission yeast and human. Science 1997; 277: 955–9
  • Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddie SD. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 1997; 90: 785–95
  • Harrington L, Zhou W, McPhail T, Oulton R, Yeung DS, Mar V. Human telomerase contains evolutionarily conserved catalytic and structural subunits. Genes Dev 1997; 11: 3109–15
  • Nakayama J, Tahara H, Tahara E, Saito M, Ito K, Nakamura H. Telomerase activation by hTRT in human normal fibroblasts and hepatocellular carcinomas. Nat Genet 1998; 18: 65–8
  • Weinrich SL, Pruzan R, Ma L, Ouellette M, Tesmer VM, Holt SE. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat Genet 1997; 17: 498–502
  • Beattie TL, Zhou W, Robinson MO, Harrington L. Re-constitution of human telomerase activity in vitro. Curr Biol 1998; 8: 177–80
  • Beattie TL, Zhou W, Robinson MO, Harrington L. Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Mol Biol Cell 2000; 11: 3329–40
  • Bryan TM, Goodrich KJ, Cech TR. Telomerase RNA bound by protein motifs specific to telomerase reverse transcriptase. Mol Cell 2000; 6: 493–9
  • Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp Cell Res 1961; 25: 585–621
  • Choo CK, Ling MT, Chan KW, Tsao SW, Zheng Z, Zhang D. Immortalization of human prostate epithelial cells by HPV 16 E6/E7 open reading frames. Prostate 1999; 40: 150–8
  • Shay JW, Wright WE, Werbin H. Defining the molecular mechanisms of human cell immortalization. Biochim Biophys Acta 1991; 1072: 1–7
  • Sedivy JM. Can ends justify the means?: telomeres and the mechanisms of replicative senescence and immortalization in mammalian cells. Proc Natl Acad Sci U S A 1998; 95: 9078–81
  • Harley CB, Futcher AB, Greider CW. Telomeres shorten during ageing of human fibroblasts. Nature 1990; 345: 458–60
  • Harley CB, Kim NW, Prowse KR, Weinrich SL, Hirsch KS, West MD. Telomerase, cell immortality, and cancer. Cold Spring Harb Symp Quant Biol 1994; 59: 307–15
  • Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J 1992; 11: 1921–9
  • Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL. Specific association of human telomerase activity with immortal cells and cancer. Science 1994; 266: 2011–5
  • Counter CM, Hirte HW, Bacchetti S, Harley CB. Telomerase activity in human ovarian carcinoma. Proc Natl Acad Sci U S A 1994; 91: 2900–4
  • Shay JW, Bacchetti S. A survey of telomerase activity in human cancer. Eur J Cancer 1997; 33: 787–91
  • Avilion AA, Piatyszek MA, Gupta J, Shay JW, Bacchetti S, Greider CW. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Res 1996; 56: 645–50
  • Ramakrishnan S, Eppenberger U, Mueller H, Shinkai Y, Narayanan R. Expression profile of the putative catalytic subunit of the telomerase gene. Cancer Res 1998; 58: 622–5
  • Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB. Extension of life-span by introduction of telomerase into normal human cells. Science 1998; 279: 349–52
  • Vaziri H, Benchimol S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr Biol 1998; 8: 279–82
  • Counter CM, Meyerson M, Eaton EN, Euisen LW, Caddie SD, Haber DA. Telomerase activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase. Oncogene 1998; 16: 1217–22
  • Yang J, Chang E, Cherry AM, Bangs CD, Oei Y, Bodnar A. Human endothelial cell life extension by telomerase expression. J Biol Chem 1999; 274: 26141–8
  • Dickson MA, Hahn WC, Ino Y, Ronfard V, Wu JY, Weinberg RA. Human keratinocytes that express hTERT and also bypass a p16(INK4a)-enforced mechanism that limits life span become immortal yet retain normal growth and differentiation characteristics. Mol Cell Biol 2000; 20: 1436–47
  • Jiang XR, Jimenez G, Chang E, Frolkis M, Kusler B, Sage M. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype. Nat Genet 1999; 21: 111–4
  • Morales CP, Holt SE, Ouellette M, Kaur KJ, Yan Y, Wilson KS. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase. Nat Genet 1999; 21: 115–8
  • Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 1998; 396: 84–8
  • Hooijberg E, Ruizendaal JJ, Snijders PJ, Kueter EW, Walboomers JM, Spits H. Immortalization of human CD8(+) T cell clones by ectopic expression of telomerase reverse transcriptase. J Immunol 2000; 165: 4239–45
  • Migliaccio M, Amacker M, Just T, Reichenbach P, Valmori D, Cerottini JC. Ectopic human telomerase catalytic subunit expression maintains telomere length but is not sufficient for CD8(+) T lymphocyte immortalization. J Immunol 2000; 165: 4978–84
  • Kilian A, Bowtell DDL, Abud HE, Hime GR, Venter OJ, Keese PK. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum Mol Genet 1997; 6: 2011–9
  • Counter CM, Botelho FM, Wang P, Harley CB, Bacchetti S. Stabilization of short telomeres and telomerase activity accompany immortalization of Epstein-Barr virus-transformed human B lymphocytes. J Virol 1994; 68: 3410–4
  • Klingelhutz AJ, Barber SA, Smith PP, Dyer K, McDougall JK. Restoration of telomeres in human papillomavirus-immortalized human anogenital epithelial cells. Mol Cell Biol 1994; 14: 961–9
  • Counter CM, Hahn WC, Wei W, Caddie SD, Beijersbergen RL, Lansdorp PM. Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization. Proc Natl Acad Sci USA 1998; 95: 14723–8
  • Halvorsen TL, Leibowitz G, Levine F. Telomerase activity is sufficient to allow transformed cells to escape from crisis. Mol Cell Biol 1999; 19: 1864–70
  • Zhu J, Wang H, Bishop JM, Blackburn EH. Telomerase extends the lifespan of virus-transformed human cells without net telomere lengthening. Proc Natl Acad Sci U S A 1999; 96: 3723–8
  • Hahn WC, Counter CM, Lundberg AS, Beijersbergen RL, Brooks MW, Weinberg RA. Creation of human tumour cells with defined genetic elements. Nature 1999; 400: 464–8
  • Elenbaas B, Spirio L, Koerner F, Fleming MD, Zimonjic DB, Donaher JL. Human breast cancer cells generated by oncogenic transformation of primary mammary epithelial cells. Genes Dev 2001; 15: 50–65
  • Ulaner GA, Hu JF, Vu TH, Giudice LC, Hoffman AR. Telomerase activity in human development is regulated by human telomerase reverse transcriptase (hTERT) transcription and by alternate splicing of hTERT transcripts. Cancer Res 1998; 58: 4168–72
  • Kharbanda S, Kumar V, Dhar S, Pandey P, Cheh C, Majumder P. Regulation of the hTERT telomerase catalytic subunit by the c-Abl tyrosine kinase. Curr Biol 2000; 10: 568–75
  • Kang SS, Kwon T, Kwon DY, Do SI. Akt protein kinase enhances human telomerase activity through phosphorylation of telomerase reverse transcriptase subunit. J Biol Chem 1999; 274: 13085–90
  • Li H, Zhao L, Yang Z, Funder JW, Liu JP. Telomerase is controlled by protein kinase Calpha in human breast cancer cells. J Biol Chem 1998; 273: 33436–42
  • Buchkovich KJ, Greider CW. Telomerase regulation during entry into the cell cycle in normal human T cells. Mol Biol Cell 1996; 7: 1443–54
  • Hu BT, Lee SC, Marin E, Ryan DH, Insel RA. Telomerase is up-regulated in human germinal center B cells in vivo and can be re-expressed in memory B cells activated in vitro. J Immunol 1997; 159: 1068–71
  • Lansdorp PM, Poon S, Chavez E, Dragowska V, Zijlmans M, Bryan T. Telomeres in the haemopoietic system. Ciba Found Symp 1997; 211: 209–18
  • Weng NP, Granger L, Hodes RJ. Telomere lengthening and telomerase activation during human B cell differentiation. Proc Natl Acad Sci U S A 1997; 94: 10827–32
  • Weng N, Levine BL, June CH, Hodes RJ. Regulation of telomerase RNA template expression in human T lymphocyte development and activation. J Immunol 1997; 158: 3215–20
  • Bryce LA, Morrison N, Hoare SF, Muir S, Keith WN. Mapping of the gene for the human telomerase reverse transcriptase, hTERT, to chromosome 5pl5.33 by fluorescence in situ hybridization. Neoplasia 2000; 2: 197–201
  • Zhang A, Zheng C, Lindvall C, Hou M, Ekedahl J, Lewensohn R. Frequent amplification of the telomerase reverse transcriptase gene in human tumors. Cancer Res 2000; 60: 6230–5
  • Greenberg RA, O'Hagan RC, Deng H, Xino Q, Hann SR, Adams RR. Telomerase reverse transcriptase gene is a direct target of c-Myc but is not functionally equivalent in cellular transformation. Oncogene 1999; 18: 1219–26
  • Kyo S, Takakura M, Taira T, Kanaya T, Itoh H, Yutsudo M. Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT). Nucleic Acids Res 2000; 28: 669–77
  • Wang J, Xie LY, Allan S, Beach D, Hannon GJ. Myc activates telomerase. Genes Dev 1998; 12: 1769–74
  • Wu KJ, Grandori C, Amacker M, Simon-Vermot N, Polack A, Lingner J. Direct activation of TERT transcription by c-MYC. Nat Genet 1999; 21: 220–4
  • Gunes C, Lichtsteiner S, Vasserot AP, Englert C. Expression of the hTERT gene is regulated at the level of transcriptional initiation and repressed by Madl. Cancer Res 2000; 60: 2116–21
  • Oh S, Song YH, Yim J, Kim TK. Identification of Mad as a repressor of the human telomerase (hTERT) gene. Oncogene 2000; 19: 1485–90
  • Kyo S, Takakura M, Kanaya T, Zhuo W, Fujimoto K, Nishio Y. Estrogen activates telomerase. Cancer Res 1999; 59: 5917–21
  • Klingelhutz AJ, Foster SA, McDougall JK. Telomerase activation by the E6 gene product of human papillomavirus type 16. Nature 1996; 380: 79–82
  • Stoppler H, Hartmann DP, Sherman L, Schlegel R. The human papillomavirus type 16 E6 and E7 oncoproteins dissociate cellular telomerase activity from the maintenance of telomere length. J Biol Chem 1997; 272: 13332–7
  • Oh S, Song Y, Yim J, Kim TK. The Wilms' tumor 1 tumor suppressor gene represses transcription of the human telomerase reverse transcriptase gene. J Biol Chem 1999; 274: 37473–8
  • Cong YS, Bacchetti S. Histone deacetylation is involved in the transcriptional repression of hTERT in normal human cells. J Biol Chem 2000; 275: 35665–8
  • Hahn WC, Stewart SA, Brooks MW, York SG, Eaton E, Kurachi A. Inhibition of telomerase limits the growth of human cancer cells. Nat Med 1999; 5: 1164–70
  • Zhang X, Mar V, Zhou W, Harrington L, Robinson MO. Telomere shortening and apoptosis in telomerase-inhibited human tumor cells. Genes Dev 1999; 13: 2388–99
  • Bisoffi M, Chakerian AE, Fore ML, Bryant JE, Hernandez JP, Moyzis RK. Inhibition of human telomerase by a retrovirus expressing telomeric antisense RNA. Eur J Cancer 1998; 34: 1242–9
  • Herbert B, Pitts AE, Baker SI, Hamilton SE, Wright WE, Shay JW. Inhibition of human telomerase in immortal human cells leads to progressive telomere shortening and cell death. Proc Natl Acad Sci U S A 1999; 96: 14276–81
  • Shammas MA, Simmons CG, Corey DR, Reis RJ. Telomerase inhibition by peptide nucleic acids reverses ‘immortality’ of transformed human cells. Oncogene 1999; 18: 6191–200
  • Vonderheide RH, Hahn WC, Schultze JL, Nadler LM. The telomerase catalytic subunit is a widely expressed tumor-associated antigen recognized by cytotoxic T lymphocytes. Immunity 1999; 10: 673–9
  • Minev B, Hipp J, Firat H, Schmidt JD, Langlade-Demoyen P, Zanetti M. Cytotoxic T cell immunity against telomerase reverse transcriptase in humans. Proc Natl Acad Sci USA 2000; 97: 4796–801
  • Nair SK, Heiser A, Boczkowski D, Majumdar A, Naoe M, Lebkowski JS. Induction of cytotoxic T cell responses and tumor immunity against unrelated tumors using telomerase reverse transcriptase RNA transfected dendritic cells. Nat Med 2000; 6: 1011–7
  • Bryan TM, Englezou A, Dalla-Pozza L, Dunham MA, Reddel RR. Evidence for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines. Nat Med 1997; 3: 1271–4
  • Lundblad V, Blackburn EH. An alternative pathway for yeast telomere maintenance rescues estl- senescence. Cell 1993; 73: 347–60
  • Thomas M, Yang L, Hornsby PJ. Formation of functional tissue from transplanted adrenocortical cells expressing telomerase reverse transcriptase. Nat Biotechnol 2000; 18: 39–42

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