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- , IMR-32, WI-38, and SaNar cells were obtained from the American Type Culture Collection Cell Repository, Rockville, Md.; all antisera used were from Meloy Biologicals and were goat antihuman heavy chain specific gamma 2 fraction, 1 mg ab/ml; ampholyte solution was Ampholine 1809–101, pH range 3.5 to 9.5 from LKB Instruments, Inc
- , IMR-32 neuroblastoma cells were killed by lymphocytes from a patient with neuroblastoma in our laboratory (unpublished data); WI-38 fibroblast cells are used routinely as normal control cells in cytotoxicity experiments and can be killed by appropriately sensitized lymphocytes