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Original Article

Membrane-associated Proteins Regulating the Complement System: Functions and Deficiencies

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Pages 87-101 | Received 22 Mar 1991, Published online: 10 Jul 2009

References

  • Bordet J. Les serums hemolytiques, les anti-toxins et les théories des serums cytoliques. Ann. de l'Institut Pasteur 1900; 14: 257
  • Yamamoto K. Lytic activity of C5-9 complexes for erythrocytes from the species other than sheep: C9 rather than C8-dependent variation in lytic activity. J. Immunol. 1977; 119: 1482
  • Brown S. J., Ramsey J., Hammer C. H., Frank M. M. Surface modulation of classical pathway activation: C2 and C3 convertase formation and regulation on sheep, guinea pig, and human erythrocytes. J. Immunol. 1983; 131: 403
  • Hänsch G. M., Hammer C. H., Vanguri E., Shin M. L. Homologous species restriction in lysis of erythrocytes by terminal complement proteins. Proc. Natl. Acad. Sci. USA 1981; 78: 5118
  • Houle J. J., Hoffman E. M. Evidence for restriction of the ability of complement to lyse homologous erythrocytes. J. Immunol. 1984; 133: 1444
  • Shin M. L., Hänsch G. M., Hu V. W., Nicholson-Weller A. Membrane factors responsible for homologous species restriction of complement-mediated lysis: evidence for a factor other than DAF operating at the stage of C8 and C9. J. Immunol. 1986; 136: 1777
  • Fearon D. T. Identification of the membrane glycoprotein that is the C3b receptor of the human erythrocyte, polymorphonuclear leucocyte, B lymphocyte and monocyte. J. Exp. Med. 1980; 125: 20
  • Nicholson-Weller A., Burge J., Fearon D. T., Weller P. E., Austen K. F. Isolation of a human erythrocyte membrane glycoprotein with decay accelerating activity for C3-convertase of the complement system. J. Immunol. 1982; 129: 184
  • Hoffmann E. M. Inhibition of complement by a substance isolated from human erythrocytes. I. Extraction from human erythrocyte stromata. Immunochemistry 1969; 6: 391
  • Schönermark S., Rauterberg E. W., Shin M. L., Loke S., Roelcke D., Hänsch G. M. Homologous species restriction in lysis of human erythrocytes: a membrane derived protein with C8-binding capacity functions as an inhibitor. J. Immunol. 1986; 136: 1772
  • Zalman L. S., Wood L. M., Müller-Eberhard H. J. Isolation of a human erythrocyte membrane protein capable of inhibiting expression of homologous complement transmembrane channels. Proc. Natl. Acad. Sci. USA 1986; 83: 6975
  • Cole J., Haisly G. A., Dykman T. R., MacDemott R. R., Atkinson J. P. Identification of an additional class of C3-binding membrane proteins of human peripheral blood leucocytes and cell lines. Proc. Natl. Acad. Sci. USA 1985; 83: 859
  • Liszewski M. K., Post T. W., Atkinson J. P. Membrane Cofactor Protein (MCP or CD46): Newest member of the regulators of complement activation gene cluster. Annu. Rev. Immunol. 1991; 9: 431
  • Davies A., Simmons D. L., Hale G., Harrison R. A., Tighe H., Lachmann P. J., Waldmann H. CD59, an Ly-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. J. Exp. Med. 1989; 170: 637
  • Sugita Y., Nakano Y., Tamita M. Isolation from human erythrocytes of a new membrane protein which inhibits the formation of complement transmembrane channels. J. Biochem. 1988; 104: 633
  • Okada N., Harada R., Fujita T., Okada H. Monoclonal antibodies capable of causing hemolysis of neuraminidase-treated human erythrocytes by homologous complement. J. Immunol. 1989; 143: 2262
  • Holguin M. H., Frederick L. R., Bernshaw N. J., Wilcox L. A., Parker C. J. Isolation and characterization of a membrane protein from normal human erythrocytes that inhibits reactive lysis of the erythrocytes of paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 1989; 84: 7
  • Whitlow M. B., Iida K., Stefanova I., Bernhard A., Nussenzweig V. H19, a surface membrane molecule involved in T-cell activation, inhibits channel formation by human complement. Cell Immunol. 1990; 126: 176
  • Schreiber A. D. Paroxysmal nocturnal hemoglobinuria revisited. N. Engl. J. Med. 1983; 309: 723
  • Rosse W. F. The control of complement activation by the blood cells in paroxysmal nocturnal hemoglobinuria. Blood 1986; 67: 268
  • Devine D. V., Gluck W. L., Rosse W. E., Weinberg J. B. Acute lymphoblastic leukemia in paroxysmal nocturnal hemoglobinuria. Evidence of evolution from the abnormal paroxysmal nocturnal hemoglobinuria clone. J. Clin. Invest. 1987; 79: 314
  • Blaas R., Weber S., Hänsch G. M., Peter H. H. Paroxysmale nachtliche Hamoglobinurie. Klin. Wochenschr. 1990; 68: 247
  • Parker C. J., Baker P. J., Rosse W. F. Increased enzymatic activity of the alternative pathway convertase when bound to the erythrocytes of paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 1982; 69: 337
  • Hänsch G. M., Hammer C., Jiji R., Rother U., Shin M. L. Lysis of paroxysmal nocturnal hemoglobinuria erythrocytes by acid-activated serum. Immunobiol. 1983; 164: 118
  • Hu V. W., Nicholson-Weller A. Enhanced complement-mediated lysis of type III paroxysmal nocturnal hemoglobinuria erythrocytes involves increased C9 binding and polymerization. Proc. Natl. Acad. Sci. USA 1985; 82: 5520
  • Nicholson-Weller J. R., March S., Rosenfeld I., Austen K. F. Affected erythrocytes of patients with paroxysmal nocturnal hemoglobinuria are deficient in the complement regulatory protein, decay accelerating factor. Proc. Natl. Acad. Sci. USA 1983; 80: 5066
  • Pangburn M. K., Schreiber R. D., Trombold J. S., Müller-Eberhard H. J. Paroxysmal nocturnal hemoglobinuria: deficiency in factor H-like functions of the abnormal erythrocytes. J. Exp. Med. 1971; 157: 1983
  • Hänsch G. M., Schönermark S., Roelcke D. Paroxysmal nocturnal hemoglobinuria type III: Lack of an erythrocyte membrane protein restricting the lysis by C5b-9. J. Clin. Invest. 1987; 80: 7
  • Zalman L. S., Wood L. M., Frank M. M., Müller-Eberhard H. J. Deficiency of the homologous restriction factor in paroxysmal nocturnal hemoglobinuria. J. Exp. Med. 1987; 165: 572
  • Okada N., Harada R., Okada H. Erythrocytes of patients with paroxysmal nocturnal haemoglobinuria acquire resistance to complement attack by purified 20-kD homologous restriction factor. Clin. Exp. Immunol. 1990; 80: 109
  • Weiler J. M., Daha M. R., Austen K. E., Fearon D. T. Control of the amplification convertase of complement by the plasma protein B1H. Proc. Natl. Acad. Sci. USA 1976; 73: 3268
  • Whaley K., Ruddy S. Modulation of the alternative complement pathway by B1H globulin. J. Exp. Med. 1976; 144: 1147
  • Shin M. L., Gelfand M. C., Nagle R. B., Carlo J. R., Green I., Frank M. M. Localization of receptors for activated complement on visceral epithelial cells of the human renal glomerulus. J. Immunol. 1977; 118: 869
  • Lublin D. M., Atkinson J. P. Decay-accelerating factor: biochemistry, molecular biology, and function. Ann. Rev. Immunol. 1989; 7: 35
  • Hourcade D., Holers V. M., Atkinson J. P. The regulators of complement activation (RCA) gene clusters. Adv. Immunol. 1989; 45: 381
  • Davitz M. A., Low N. G., Nussenzweig V. Release of decay accelerating factor (DAF) from the cell membrane by phosphatidylinositol specific phospholipase C (PIPLC). J. Exp. Med. 1986; 163: 1150
  • Medof M. F., Kinoshita T., Nussenzweig V. Inhibition of complement activation on the surface of cells after incorporation of decay-accelerating factor (DAF) into their membranes. J. Exp. Med. 1984; 160: 1558
  • Lublin D. M., Coyne K. E. Transfected decay-accelerating factor and membrane cofactor protein protect foreign cells from complement-mediated damage: equal efficiency of phospholipid-anchored and transmembrane forms. Complement Inflamm. 1991; 8: 184, (abstract).
  • Atkinson J. R., Oglesby T. I., White D., Adams E. A., Liszewski M. K. Separation of self from non-self in the complement system: a role for membrane cofactor protein and decay accelerating factor. Clin. Exp. Immunol. 1991; 86: 27
  • Cosio F. G., Sedmak D. D., Mahan I. D., Nahman N. S. Localization of decay accelerating factor in normal and diseased kidneys. Kidney Int. 1989; 36: 100
  • Shibata T., Cosio F. G. Expression of cell-associated C3 regulatory proteins (CCRP) on human glomerular cells. JASN 1990; 1: 538
  • Rother U., Hänsch G. M., Menzel J., Rother K. Deviated lysis: transfer of complement lytic activity to unsensitized cells. I. Generation of the transferable activity on the surface of complement resistant bacteria. Z. Immun.-Forsch. 1974; 148: 172
  • Rother U., Hänsch G. M., Rother K. Deviated lysis: transfer of complement lytic activity to unsensitized cells. II. Generation of the activity by inulin and by antigen antibody complexes. Z. Immun. Forsch. 1976; 151: 442
  • Götze O., Müller-Eberhardt H. J. Lysis of erythrocytes by complement in the absence of antibody. J. Exp. Med. 1970; 132: 898
  • Thompson R. A., Rowe D. S. Reactive haemolysis—a distinctive form of red cell lysis. Immunology 1968; 14: 745
  • Rother U., Hänsch G. M., Rauterberg E. W., Jungfer H., Rother K. Deviated lysis: lysis of unsensitized cells by complement. V. Generation of the activity by low pH or low ionic strength. Z. Immun. Forsch. 1978; 155: 118
  • Vogt W., Zabern I. V., Hesse D., Nolte R., Haller Y. Generation of an activated form of C5 (C5b like C5) by oxygen radicals. Immunology letters 1986; 14: 209, /87.
  • Kirschfink M., Borsos T. Binding and activation of C4 and C3 on the red cell surface by non-complement enzymes. Molecular Immunology 1988; 25: 505
  • Podack E. R., Kolb W. P., Müller-Eberhard H. J. The C5b6 complex: formation, isolation and inhibition of its activity by lipoprotein and the S-protein of human serum. J. Immunol. 1978; 120: 1841, 49.
  • Jenne D., Stanley K. K. Molecular cloning of S-protein, a link between complement, coagulation and cell-substrate adhesion. EMBO J. 1985; 4: 3353
  • Lint T. E., Behrends C. L., Gewurz H. Serum lipoproteins and C567-INH activity. J. Immunol. 1977; 119: 883
  • Murphy B. E., Kirszbaum L., Walker I. D., d'Apice A., SP- J. P. J. Clin. Invest. 1988; 81: 1858, 40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis.
  • Murphy B. F., Saunders J. R., O'Bryan M. K., Kirszbaum L., Walker I. D., d'Apice A. J. F. SP-40,40 is an inhibitor of C5b-6 initiated hemolysis. Int. Immunol. 1989; 1: 551
  • Schönermark S., Filsinger S., Berger B., Hänsch G. M. The C8-binding protein of human erythrocytes: interaction with the components of the complement-attack phase. Immunology 1988; 63: 585
  • Young J. D., Rosse W. F., Hasselkus-Light C. S. The homologous restriction factor is immunologically related to complement components C8 and C9 and to lymphocyte pore-forming protein perforin through cysteine-rich domains. Biochem. Biophys. Res. Comm. 1990; 166: 648
  • Yamamoto H., Blaas R., Nicholson-Weller A., Hansen G. M. Homologous species restriction of the complement-mediated killing of nucleated cells. Immunology 1990; 70: 422–426
  • Meri S., Morgan B. R., Davies A., Daniels R. H., Olavesen M. G., Waldmann H., Lachmann P. J. Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers. Immunology 1990; 71: 1
  • Zhao J., Rollins S. A., Bothwell A. M., Sims PJ. Amplified gene expression in CD59-transfected CHO cells confers protection against the membrane attack complex of human complement. FASEB J., 5: A1339, 1991 (abstract).
  • Blaas R., Berger B., Weber S., Peter H. H., Hänsch G. M. Paroxysmal nocturnal hemoglobinuria. Enhanced stimulation of platelets by the terminal complement components is related to the lack of C8bp in the membrane. J. Immunol. 1988; 140: 3045–51
  • Schieren G., Schönermark M., Wagner C., Blaas-Mautner P., Rother K., Hänsch G. M. Defense mechanisms of human glomerular mesangial cells (GMC) against complement (C) attack. JASN 1990; 1: 536, (abstract).
  • Rooney I. A., Davies A., Griffiths D., Williams J. D., Davies M., Meri S., Lachmann P. J., Morgan B. P. The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells. Clin. Exp. Immunol. 1991; 83: 251
  • Meri S., Walsh L., Davies A., Tone M., Daniels R. H., Morgan B. R., Waldmann H., Lachmann W. J. Tissue distribution and function of protectin (CD59), a complement lysis restricting factor. Complement Inflamm. 1990; 7: 138, (abstract).
  • Schieren G., Blaas P., Hänsch G. M. Detection of a cytoplasmic form of C8 binding protein (C8bp)in nucleated cells and enhanced expression after stimulation with phorbol ester. Complement Inflamm. 1990; 7: 118, (abstract).
  • Schieren G., Janssen O., Hänsch G. M. Enhanced expression of the complement-regulatory factor C8 binding protein (C8bp) on U937 cells after stimulation with interleukin - 1β, endotoxin, γ-interferon or phorbol ester. J. Immunol. 1992, in press.
  • Gordon D. L., Papazakaroudakis H., Averym V., Sadlon T., Arellano A., Okada N. FMLP stimulation and increases in intracellular calcium cause upregulation of neutrophil CD59 (HRF20). Complement Inflamm. 1991; 8: 136, (abstract).
  • Zhang Z. N., Liu E. K. Clinical features of paroxysmal nocturnal hemoglobinuria (PNH) in China as compared with those in United Kingdom. Chung Hua Nei Ko Tsa Chih 1991; 30: 276
  • Ham T. H. Chronic hemolytic anemia with paroxysmal nocturnal hemoglobinuria: study of the mechanism of hemolysis in relation to acid-base equilibrium. N. Engl. J. Med. 1937; 217: 915
  • Parker C. J., Baker P. J., Rosse W. F. Comparison of binding characteristics of factor B and H to C3b on normal and paroxysmal nocturnal hemoglobinuria erythrocytes. J. Immunol. 1983; 131: 2484
  • Nicholson-Weller A., Douglas M. D., Spice D. B., Austen M. D. Deficiency of the complement regulatory protein, “decay accelerating factor”, on membranes of granulocytes, monocytes, and platelets in paroxysmal nocturnal hemoglobinuria. N. Engl. J. Med. 1985; 312: 1091
  • Devine D. V., Siegel R. S., Rosse W. F. Interactions of the platelets in paroxysmal nocturnal hemoglobinuria with complement. Relationship to defects in the regulation of complement and to platelet survival in vivo. J. Clin. Invest. 1987; 79: 131
  • Sims P. J., Rollins S. A., Wiedmer T. Regulatory control of complement on blood platelets. Modulation of blood platelet procoagulant responses by a membrane inhibitor of the C5b-9 complex. J. Biol. Chem. 1922; 264: 8, 1989
  • Stern M., Rosse W. F. Two populations of granulocytes in paroxysmal nocturnal hemoglobinuria. Blood 1979; 53: 928
  • Oni S. B., Osunkoya B. O., Kuzzatto L. Paroxysmal nocturnal hemoglobinuria: evidence for monoclonal origin of abnormal red cells. Blood 1970; 36: 145
  • Packmann C. H., Rosenfeld S. I., Jenkins D. E., Thiem P. A., Leddy J. P. Complement lysis of human erythrocytes: differing susceptibility of two types of paroxysmal nocturnal hemoglobinuria cells to C5b-9. J. Clin. Invest. 1979; 64: 428
  • Chow F. L., Hall S. E., Rosse W. F., Telen M. J. Separation of the acetylcholinesterase-deficient red cells in paroxysmal nocturnal hemoglobinuria. Blood 1986; 67: 893
  • Shichishima T., Terasawa T., Hashimoto C., Ohto H., Uchida T., Maruyama Y. Heterogenous expression of decay accelerating factor and CD59/membrane attack complex inhibition factor on paroxysmal nocturnal haemoglobinuria (PNH) erythrocytes. Br. J. Haematol. 1991; 78: 545
  • Rosse W. E., Hoffman S., Campbell M., Borowitz M., Moore J. Q., Parker C. J. The erythrocytes in paroxysmal nocturnal haemoglobinuria of intermediate sensitivity to complement lysis. Br. J. Haematol. 1991; 79: 99
  • Kawakami Z., Ninomiya H., Tomiyama J., Abe T. Deficiency of glycosyl-phosphatidylinositol anchored proteins on paroxysmal nocturnal haemoglobinuria (PNH) neutrophils and monocytes: heterogeneous deficiency of decay-accelerating factor (DAF) and CD16 on PNH neutrophils. Br. J. Haematol. 1990; 74: 508
  • Selvaraj P., Dustin M. L., Silber R., Low M. G., Springer T. A. Deficiency of lymphocyte-function-associated antigen 3 (LFA-3) in paroxysmal nocturnal hemoglobinuria. Functional correlated and evidence for a phosphatidylinositol membrane anchor. J. Exp. Med. 1987; 166: 1011
  • Selvaraj P., Rosse W. E., Silber R., Springer T. A. The major Fc receptor in blood has a phosphatidylinositol anchor and is deficient in paroxysmal nocturnal haemoglobinuria. Nature 1988; 333: 565
  • Simmons D. L., Tan S., Tenen D. G., Nicholson-Weller A., Seed B. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood 1989; 73(1)284
  • Hänsch G. M., Weller P. F., Nicholson-Weller A. Release of C8bp by phosphatidylinositol-specific phospholipase C. Blood 1988; 72: 1089
  • Lewis S. M., Dacie J. V. Neutrophil (leucocyte) alkaline phosphatase in paroxysmal nocturnal hemoglobinuria. Br. J. Haematol. 1965; 11: 549
  • Auditore J. V., Hartmann R. C., Flexner J. M., Balchum O. J. The erythrocyte acetylcholinesterase enzyme in paroxysmal nocturnal hemoglobinuria. Arch. Pathol. 1960; 69: 534
  • Chow F. L., Telen M. J., Rosse W. F. The acetylcholinesterase in paroxysmal nocturnal hemoglobinuria: Evidence that the enzyme is absent from the cell membrane. Blood 1985; 66: 940
  • Stafford H. A., Tykocinski M. L., Lublin D. M., Holers V. M., Rosse W. F., Atkinson J. R., Medof M. E. Normal polymorphic variations and transcription of the decay accelerating factor gene in paroxysmal nocturnal hemoglobinuria cells. Proc. Natl. Acad. Sci. USA 1988; 85: 880
  • Moore J. C., Frank M. M., Müller-Eberhard H. J., Young N. S. Decay accelerating factor is present in PNH erythroid progenitors and lost during erythropoiesis in vitro. J. Exp. Med. 1985; 162: 1182
  • Nakakuma H., Kawaguchi T., Horikawa K., Hidaka M., Yonemura Y., Kawakita M., Kagimoto T., Iwamori M., Nagai Y., Takatsuki K. Altered expression of gangliosides in erythrocytes of paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 1990; 85: 1456
  • Medof M. E., Gottlieb A., Kinoshita T., Hall S., Silber R., Nussenzweig V., Rosse W. F. Relationship between decay accelerating factor deficiency, diminished acetylcholinesterase activity, and defective terminal complement pathway restriction in paroxysmal nocturnal hemoglobinuria erythrocyte. J. Clin. Invest. 1987; 80: 165
  • Parker C. J., Soldato C. M., Rosse W. F. Abnormality of glycophorin-α on paroxysmal nocturnal hemoglobinuria erythrocytes. J. Clin. Invest. 1984; 73: 1130
  • Yamashina M., Ueda E., Kinoshita T., Takami T., Ojima A., Ono H., Tanaka H., Kondo N., Orii T., Okada N., Okada H., Luone K., Kitani T. Inherited complete deficiency of 20 kilodalton homologous restriction factor (CD59) as a cause of paroxysmal nocturnal hemiglobinuria. N. Engl. J. Med. 1990; 323: 1184
  • Noda M., Yoon K., Rodon G. A., Koppel D. E. High lateral mobility of endogenous and transfected alkaline phosphatase: a phosphatidyl-inositol-anchored membrane protein. J. Cell Biol. 1987; 105: 1671
  • Altman A., Mustelin T., Coggeshall K. M. T lymphocyte activation: a biological model of signal transduction. Critical Reviews in Immunology 1990; 10: 347
  • Stiernberg J., Low M. G., Flaherty L., Kincade P. W. Removal of lymphocyte surface molecules with phosphatidyl-inositol-specific phospholipase C: Effects on mitogen responses. J. Immunol. 1987; 138: 3877
  • Tomiyama J., Ninomiya H., Abe T. Enhanced complement susceptibility and dysfunction of lymphocytes in paroxysmal nocturnal haemoglobinuria (PNH). Br. J. Haematol. 1990; 76: 540
  • Davis L. S., Patel S. S., Atkinson J. P., Lipsky P. E. Decay-accelerating factor functions as a signal transducing molecule for human T cells. J. Immunol. 1988; 141: 2246
  • Rock K. L., Yeh E. T. H., Gramm C. F., Haber S. I., Reiser H., Benacerraf B. TAP, a novel T cell-activating protein involved in the stimulation of MHC-restricted T lymphocytes. J. Exp. Med. 1986; 163: 315
  • Levy L., Lepow I. H. Assay and properties of serum inhibitor of C1-esterase. Proc. Soc. Exp. Biol. Med. 1959; 101: 608
  • Scharfstein J., Ferreira A., Gigli I., Nussenzweig V. Human C4-binding protein, I. Isolation and characterization. J. Exp. Med. 1978; 148: 207
  • Nelson R. A., Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea pig serum. Immunochemistry 1966; 3: 111
  • Nilsson V. R., Müller-Eberhard H. J. Isolation of BIF-globulin from human serum and its characterization as the fifth component of complement. J. Exp. Med. 1965; 122: 277
  • Pangburn M. K., Schreiber R. D., Müller-Eberhard H. J. Human complement C3b inactivator: isolation, characterization, and demonstration of an absolute requirement for the serum protein β1H for cleavage of C3b and C4b in solution. J. Exp. Med. 1977; 146: 257
  • Quigg R. J. Isolation of a novel complement regulatory protein (GCRP) from cultured rat glomerular epithelial cells (GEC). JASN 1990; 1: 535, (abstract).
  • Watts M. J., Dankert J. R., Morgan B. F. Isolation and characterization of a membrane-attack-complex-inhibiting protein present in human serum and other biological fluids. Biochem. J. 1990; 265: 471
  • Brauch H., Roelcke D., Rother U. Glycophorin A inhibits lysis by the complement attack phase. Immunobiol. 1983; 165: 115
  • Schieren G., Hänsch G. M. CD59 and C3 binding protein (C8bp) are biochemically distinct proteins. Complement Inflamm. 1991; 8: 219, (abstract).
  • Simmons D. L., Tan S., Tenen D. G., Nicholson-Weller A., Seed B. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood 1989; 73: 284
  • Van der Schoot C. E., Huizinga T. W., van-'t Veer-Korthof E. T., Wijmans R., Pinkster J., von dem Borne A. E. Deficiency of glycosyl-phosphatidylinositol-linked membrane glycoproteins of leukocytes in paroxysmal nocturnal hemoglobinuria, description of a new diagnostic cytofluorometric assay. Blood 1990; 76: 1853
  • Schubert J., Uciechowski P., Delany R., Tischler H. J., Kolanus W., Schmidt R. E. The PIG-anchoring defect in NK lymphocytes of PNH patients. Blood 1990; 76: 1181
  • Jost C. R., Gaillard M. L., Fransen J. A. M., Daha MR., Ginsel L. A. Intracellular localization of glycosyl-phosphatidylinositol-anchored CD67 and FcRIII (CD16) in affected neutrophil granulocytes of patients with paroxysmal nocturnal hemoglobinuria. Blood 1991; 78: 3030
  • Nakakuma H., Kawaguchi T., Horikawa K., Hidaka M., Yonemura Y., Kawakita M., Kagimoto T., Iwamori M., Nagai Y., Takatsuki K. Altered expression of gangliosides in erythrocytes of paroxysmal nocturnal hemoglobinuria. J. Clin. Invest. 1990; 85: 1456
  • Tsien R. Y., Pozzan T., Rink T. J. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. The Journal of Cell Biology 1982; 94: 326

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