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Original Article

Adenylate Cyclase Responses and Biochemical Characterization of Primary Rabbit Proximal Tubular Cell Cultures and Llc-Pk1 Cells

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Pages 177-182 | Published online: 07 Jul 2009

References

  • Husted R F, Welsch M J, Stokes JB. Variability of functional characteristics of MDCK cells. Am J Physiol 1986; 250: C214–C221
  • Wohlwend A, Vassa UID, Belin D, Orci L. LLC-PK1 cells: cloning of phenotypically stable subpopulations. Am J Physiol 1986; 250: C682–C687
  • Haggerty J G, Agarwal N, Cragoe E J, Adelberg E A, Slayman C W. LLC-PK1 mutant with increased Na+-H+ exchange and decreased sensitivity to amiloride. Am J Physiol 1988; 255: C495–C501
  • Toutain H, Fillastre J P, Morin J P. Preparative free flow electrophoresis for the isolation of two populations of proximal cells from the rabbit kidney. Eur J Cell Biol 1989; 49: 274–280
  • Gstraunthaler G, Pfaller W, Kotanko P. Biochemical characterization of renal epithelial cell cultures (LLC-PK1 and MDCK). Am J Physiol 1985; 248: F536–F544
  • Brendel K, Meezan E. Isolation and properties of a pure preparation of proximal kidney tubules obtained without collagenase treatment. Fed Proc 1975; 34: 803
  • Bradford MM. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976; 72: 248–254
  • Hjelle J T, Morin JP, Trouet A. Analytical cell fractionation of isolated rabbit renal proximal tubules. Kidney Int 1981; 20: 71–77
  • Bergmeyer HU. Hexokinase assay. Methods of enzymatic analysis, H.U. Bergmeyer. Academic Press, New York 1974; pp 473–475
  • Bergmeyer H U, Bemt E. Lactate dehydrogenase UV-assay with pyruvate and NADH. Methods of enzymatic analysis, H.U. Bergmeyer. Academic Press, New York 1974; pp 574–579
  • Aebi H. Catalase UV assay. Methods of enzymatic analysis, H.U. Bergmeyer. Academic Press, New York 1974; pp 674–678
  • Habig WH, Pabst M J, Jakobi W B. Glutathion-S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130–7139
  • Pennington RJ. Biochemistry of dystrophic muscle: mitochondrial succinate tetrazolium reductase and adenosine triphosphatase. Biochem J 1961; 80: 649–654
  • Torikai S, Wang M S, Klein K L, Kurokawa K. Adenylate cyclase and cell cyclic AMP of rat cortical thick ascending limb of Henle. Kidney Int 1981; 20: 649–654
  • Gilman A. A protein binding assay for adenosin-3′-5′-cyclic monophosphate. Proc Natl Acad Sci USA 1970; 67: 305–312
  • Levine M A, Downs R W, Singer M, Marx S J, Aurback G D, Spiegel A M. Deficient activity of guanine nucleotide regulatory protein in erythrocytes from patients with pseudohypoparathyrodism. Biochem Biophys Res Commun 1980; 94: 1318–1324
  • Lowry O H, Rosebrough N J, Fan AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265–275
  • Burch H B, Narins R G, Chu C, Fagioli S, Choi S, Mc Carthy W, Lowry O. Distribution along the rat nephron of three enzymes of glucone-ogenesis in acidosis and starvation. Am J Physiol 1978; 235: F246–F253
  • Ross B D, Guder W G. Heterogeneity and ccmpartmentalion in the kidney. Metabolic Compartmentation, H. Sies. Academic Press, New York 1984; pp. 363–409
  • Guder W G, Ross B D. Enzyme distribution along the nephron. Kidney Int 1984; 26: 101–111
  • Morel F. Sites of hormone action in the mammalian nephron. Am J Physiol 1981; 240: F159–F164
  • Bell C L, Tenenhouse H S, Scriver C R. Lnitiation and characterization of primary mouse kidney epithelial cultures. In Vitro Cell Develop Biol 1988; 24: 683–695
  • Chung S D, Alavi N, Livingston D, Hiller S, Taub M. Characterization of primary rabbit kidney cultures that express proximal tubule functions in a hormonally defined medium. J Cell Biol 1982; 95: 118–126
  • Trifillis A L, Regec A L, Trump B F. Isolation culture and characterization of human renal tubular cells. J Urol 1985; 133: 324–329
  • Katz A L, Doucet A, Morel F. Na+-K+-ATPase activity along the rabbit, rat and mouse nephron. Am J Physiol 1979; 237: F114–F120
  • Tang M J, Suresh K R, Tannen R L. Carbohydrate metabolism by primary cultures of rabbit proximal tubules. Am J Physiol 1989; 256: C532–C539
  • Gstraunlhaler G. Epithelial cells in tissue culture. Renal Physiol Biochem 1988; 11: 1–42
  • Bissell D M, Levine G A, Bissell M J. Glucose metabolism by adult hepatocytes in primary cultures and cell lines from rat liver. Am J Physiol 1978; 234: C122–C130

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