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Original Article

Mapping of Autoantigenic Epitopes on Recombinant Thyroid Peroxidase Fragments Using the Polymerase Chain Reaction

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Pages 257-268 | Received 04 Oct 1989, Published online: 07 Jul 2009

References

  • Weetman A P, McGregor A M. Autoimmune thyroid disease: Developments in our understanding. Endocrine Revs 1984; 5: 309–361
  • Roitt I M, Ling N R, Doniach D, Crouchman K G. The cytoplasmic autoantigen of the human thyroid, immunological and biochemical characteristics. Immunol 1964; 7: 375–393
  • Khoury E L, Hammond L, Bottazzo G F, Doniach D. Presence of organ specific microsomal autoantigen on the surface of human thyroid cells in culture: its involvement in complement mediated cytotoxicity. Clin ESP Immunol 1981; 45: 316–328
  • Forbes I J, Roitt I M, Doniach D, Soloman I L. The thyroid cytotoxic autoantibody. JClin Invest 1962; 41: 996–1006
  • Weetman A P, Gunn C A, Rennie D P, Hall R, McGregor A M. The production and characterization of monoclonal antibodies to the human thyroid microsome. J Endocrinol 1985; 105: 47–3
  • Banga J P, Mirakian R, Hammond L, Pryce G, Bidey S, Bottazzo F, Weetman A P, McGregor A M, Roitt I M. Characterization of monoclonal antibodies directed towards the microsome/microvillar thyroid autoantigen recognized by Hashimoto autoantibodies. Clin E.vp Immunol 1986; 64: 544–554
  • Banga J P, Pryce G, Hammon L, Roitt I M. Characterization of the human thyroid microsomal antigen involved in thyroid autoimmunity. Biochem Soc Trans 1984; 12: 1118–1119
  • Banga J P, Pryce G, Hammond L, Roitt I M. Structural features of the autoantigens involved in thyroid autoimmune diseasea: the thyroid microsomal/microvillar antigen. Mol Immunol 1985; 22: 629–642
  • Hamada N, Grimm C, Mori M, De Groot L J. Identification of a thyroid microsomal antigen by Western blot and immunoprecipitation. J Clin Endocrind Metab 1985; 61: 120–128
  • Kajita Y, Morgan D, Pdrkes A B, Rees Smith B. Labelling and immunoprecipitation of thyroid microsomal antigen. FEBS Letts 1985; 187: 334–338
  • Czarnocka B, Ruff J, Ferrand M, Carayon P, Lissitzky S. Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid disease. FEBS Letts 1985; 190: 147–151
  • Portman L, Hamada N, Heinrich G, De Groot L J. Anti-thyroid peroxidase antibody in patients with autoimmune thyroid disease: possible identity with anti-microsomal antibody. J Clin Endocrinol Metab 1985; 61: 1001–1004
  • Kimura S, Kotani T, McBride O M, Umeki K, Hirdi K, Nakayama T, Ohtaki S. Human thyroid peroxidase: complete cDNA and protein sequence, chromosome mapping and identification of two alternately spliced mRNAs. Proc Null Acad Sci 1987; 84: 5555–5559
  • Libert F, Ruel J, Ludgate M, Swillens S, Alexander N, Vassart G, Dinsart C. Thyroperoxidase, an autoantigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J, 6: 4193–4196
  • Seto P, Hirayu H, Magnusson R P, Gestautas J, Portman L, De Groot L J, Rapoport B. Isolation of a complementary DNA clone for thyroid microsomal antigen. J Clin Invest 1987; 80: 1205–1208
  • Kimura S, Hong Y S, Kotani T, Ohtaki S, Kikkawa F. Structure of the human thyroid peroxidase gene: comparison and relationship to the human myeloperoxidase gene. Biochem 1989; 28: 4481489
  • Benjamin D, Berzofsky J, East I, Gurd F, Hannum C, Leach S, et al. Protein antigenic structures recognized by T cells: the antigenic structure of proteins: a reappraisal. Ann Rev Immunol 1984; 2: 67–101
  • De Lisi S, Berzofsky J A. T cell antigenic sites tend to be amphipathic structures. Proc Natl Acad Sci 1985; 82: 7048–7052
  • Rothbard J B. Peptides and the cellular immune response. Ann Inst Pusfeur 1986; 137E: 518–526
  • Hopp T P, Woods K R. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acud Sci 1981; 78: 3824–3828
  • Milich D R. Synthetic T and B cell recognition sites: implications for vaccine development. Advances in Immunology, F J Dixon. Academic Press, London 1989; Vol. 45: 195–282
  • Mackay I R, Gershwin M E. Molecular basis of mitochondrial autoreactivity in primary billiary cirrhosis. Immunol Today 1989; 10: 315–318
  • Barkas T, Gabriel J M, Mauron A, Hughes G J, Roth B, Alliod C, Tzartos S J, Ballivet M. Monoclonal antibodies to the main immunogenic region of the nicotinic receptor bind to residues 61–76. of the alpha subunit. J Eiol Chem 1988; 263: 5916–5920
  • Banga J P, Barnett P S, Mahadevan D, McGregor A M. Immune recognition of antigen and its relevance to autoimmune disease: recent advances at the molecular level. Eur J Clin Invest 1989; 19: 107–116
  • Wraith D C, McDevitt H O, Steinman L, Acho-Orbea. T cell recognition as the target for immune intervention in autoimmune disease. Cell 1989; 57: 709–715
  • Doble N, Banga J P, Pope R, Lalor E, Kildu K P, McGregor A M. Autoantibodies to the thyroid microsomal/thyroid peroxidase antigen are polyclonal and directed to several distinct antigenic sites. Immunol 1988; 64: 23–29
  • Banga J P, Tomlinson R WS, Doble N, Odell E, McGregor A M. Thyroid microsomal/thyroid peroxidase autoantibodies show discrete patterns of crossreactivity to myeloperoxidase, lac-toperoxidase and horeradish peroxidase. Immunol 1989; 67: 197–204
  • Banga J P, Barnett P S, Ewins D L, Page M J, McGregor A M. Analysis of autoantigenic determinants of thyroid peroxidase antigen, generated using polymerase chain reaction. Mol. Eiol. of Autoimmune Diseuse, A G Demaine, J P Banga, A M. McGregor. Springer-Verlag, Berlin 1990, in press
  • Barnett P S, Banga J P, Watkins J, Page M J, McGregor A M. Nucleotide sequence of the alternatively spliced human thyroid peroxidase cDNA, TPO-2. Nuc Acid Res 1990; 18: 670
  • Maniatis T, Fritsh E F, Sambrook J. Molecular cloning. A laboratory Manual. Cold Spring Harbour Laboratory. 1982
  • Smith D B, Johnson K S. Single step purification of polypeptides expressed in Eschericiu coli, as fusions with glutathione S-transferase. Gene 1988; 67: 31–40
  • Marston F AO. The purification of eukaryotic polypeptides synthesized i. Eschericiu coli. Eiochem J 1986; 240: 1–12
  • Yokoyama N, Taurog A. Porcine thyroid peroxidase: relationship between the native enzyme and an active, highly purified tryptic fragment. Mol Endocrinol 1988; 2: 838–844
  • Saiki R K, Bugawau T L, Horn G T, Mullis K B, Erlich H A. Analysis of enzymatically amplified B-globin and HLA-DQ DNA with allele specific oligonucleotide probes. Nuture 1986; 324: 163–166
  • Bell J. The polymerase chain reaction. Immunol Today 1989; 10: 351–355
  • Ludgate M, Mariotti S, Libert F, Dinsart C, Piccolo P, Santini F, Ruf J, Pinchera A, Vassart G. Antibodies to human thyroid peroxidase in autoimmune thyroid disease: studies with a cloned recombinant complementary deoxyribonucleic acid epitop. J Clin Endocrinol Mefab 1989; 68: 1091–1096

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