27
Views
66
CrossRef citations to date
0
Altmetric
Original Article

Autoantibodies to the Ro/Ssa Antigen are Conformation Dependent. I: Anti-60 kD Antibodies are Mainly Directed to the Native Protein; Anti-52 kD Antibodies are Mainly Directed to the Denatured Protein

&
Pages 57-65 | Received 03 Jun 1992, Accepted 17 Jul 1992, Published online: 07 Jul 2009

References

  • Harley J B, Alexander E, Bias W, Fox O, Provost T T, Reichlin M, Yamagata H, Amett F. Anti-Ro(SS-A) and anti-La(SS-B) in patients with Sjögren's syndrome. Arthrits Rheum 1986; 29: 196–206
  • Harley J B, Sestak A L, Willis L G, Fu S M, Hansen J A, Reichlin M. A model for disease heterogeneity in systemic lupus ery-thematosus. Relationships between histocompatibility antigens, autoantibodies. and lymphopenia or renal disease. Arthritis Rheum 1989; 32: 826–836
  • Ben-Chetrit E, Chan E K, Sullivan K F, Tan E M. A 52-kD protein is a novel component of the SSA/Ro antigenic particle. J Exp Med 1988; 167: 1560–1571
  • Rader M D, O'Brien C, Liu Y, Harley J B, Reichlin M. The heterogeneity of the Ro/SSA antigen: different molecular forms in lymphocytes and red blood cells. J Clin Invest 1989; 83: 1293–1298
  • Itoh Y, Rader M D, Reichlin M. Heterogeneity of the Ro/SSA antigen and autoanti-Ro/SSA response: Evidence for 4 antigenically distinct forms. Clin Exp Immunol 1990; 81: 45–51
  • Rader M D, Codding C, Reichlin M. Different antigen fine specificities in anti-Ro(SSA) when other precipitating autoantibodies are present. Arthritis Rheum 1989; 32: 1563–1571
  • Wolin S L, Steitz J A. The Ro small cytoplasmic ribonucleo-proteins: identification of the antigenic protein and its binding site on the Ro RNAs. Proc Natl Acad Sci USA 1984; 81: 1996–2000
  • Ben-Chetrit E, Gandy B J, Tan E M, Sullivan K F. Isolation and characterization of a cDNA clone encoding the 60-kD component of the human SS-A/R0 ribonucleoprotein autoantigen. J Clin Invest 1989; 83: 1284–1292
  • Boire G, Craft J. Biochemical and immunological heterogeneity of the Ro ribonucleoprotein particles. Analysis with sera specific for the RohY5 particle. J Clin Invest 1989; 84: 270–279
  • Boire G, Craft I. Human Ro ribonucleoprotein particles: characterization of native structure and stable association with the La polypeptide. J Clin Invest 1990; 85: 1182–1190
  • Mamula M J, Silvennan E D, Laxer R M, Bentur L, Isacovics B, Hardin J A. Human monoclonal anti-La antibodies. The La protein resides on a subset of Ro particles. J Immunol 1989; 143: 2923–2928
  • Buyon J P, Slade S G, Chan E KL, Tan E M, Winchester R. Effective separation of the 52 kDa SSA/Ro polypeptide from the 48-kDa SSB/La polypeptide by altering conditions of poly-acrylamide gel electrophoresis. J Immunol Method 1990; 129: 207–210
  • Itoh K, Itoh Y, Frank M B. Protein heterogeneity in the human Ro/SSA ribonucleoproteins. The 52– and 60-kD Ro/SSA autoantigens are encoded by separate genes. J Clin Invest 1991; 87: 177–186
  • Lerner M R, Steitz J A. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Nat/Acad Sci USA 1979; 76: 5495–5499
  • Forman M S, Nakamura M, Mimori T, Gelpi C, Hardin J A. Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. Arthritis Rheum 1985; 28: 1356–1361
  • Olmstead J B. Affinity purification of antibodies for diazotized paper blots of heterogeneous protein samples. J Biol Chem 1981; 256: 11955–11957
  • Mamula M I, Fox O F, Yamagata H, Harley J B. The Ro/SSA autoantigen as an immunogen: some anti-Ro/SSA binds IgG. J Exp Med 1987; 86: 1889–1901
  • Itoh Y, Kreit J D, Reichlin M. Organ distribution of the Ro/SSA antigen in the guinea pig. Arthritis Rheum 1990; 33: 1815–1821
  • Itoh Y, Reichlin M. Ro/SSA antigen in human platelet: different distributions of the isoforrns of Ro/SSA protein and the Ro/SSA-binding RNAs. Arthritis Rheum 1991; 34: 888–893
  • Buyon J P, Ben-Chetrit E, Karp S, Roubey R AS, Pompeo L, Reeves W H, Tan E M, Winchester R. Acquired congenital hean block. Pattern of maternal antibody response to biochemically defined antigens of the SSAmo-SSB/La system in neonatal lupus. J Clin Invest 1989; 84: 627–634
  • Ben-Chetrit E, Fox R I, Tan E M. Dissociation of immune responses to the SS-A(Ro) 52-kD and 60-kD polypeptides in systemic lupus erythematosus and Sjögrens syndrome. Arthritis Rheum 1990; 33: 349–355
  • Byers D E, Itoh Y, Reichlin M. The evolution of red blood cell and lymphocyte Ro/SSA. Autoimmunity 1990; 7: 121–128
  • Itoh K, Itoh Y, Frank M B. Molecular analysis of genomic DNA encoding 52-kDa Ro/SSA and fine epitope mapping. J Clin Invest, (submitted)
  • Landschulz W H, Johnson P F, McKnight S L. The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins. Science, Washington, DC 1988; 240: 1759–1764
  • Boire G, Lopez-Longo F-J, Lapointe S, Menard H A. Sera from patients with autoimmune disease recognize conformational determinants on the 60-kD Ro/SSA protein. Arthritis Rheum 1991; 34: 722–730
  • Surh C D, Coppel R, Gershwin M E. Structural requirement for autoreactivity on human PDH-E2. the major autoantigen of primary biliary cirrhosis: implication for a conformational autoepitope. J Immunol 1990; 144: 3367–3374
  • Huff J P, Roos G, Peebles C L, Houghten R, Sullivan K F, Tan E M. Insights into native epitopes of proliferating cell nuclear antigen (PCNA), using recombinant DNA protein products. J Exp Med 1990; 172: 419–429
  • Chambers J C, Kenan D, Martin B J, Keene J D. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem 1988; 263: 18043–18051
  • St Clair E W, Pisetsky D S, Reich C F, Keene J D. Analysis of autoantibody binding to different regions of the human La antigen expressed in recombinant fusion proteins. J Immunol 1988; 141: 4173–4180
  • Kohsaka H, Yamamoto K, Fujii H, Miura H, Miyasaka M, Nishioka K, Miyamoto T. Fine epitope mapping of the human SS-B/La protein. Identification of a distinct autoepitope homologous to a viral gag polyprotein. J Clin Invest 1990; 85: 1566–1574
  • Sturgess A D, Peterson M G, McNeilage L J, Whittingham S, Coppel R L. Characteristics and epitope mapping of a cloned human autoantigen La. J Immunol 1988; 140: 3212–3218
  • Rokeach L A, Jannatipour H, Hoch S O. Heterologous expression and epitope mapping of a human small nuclear ribo-nucleoprotein-associated Sm-B'/B autoantigen. J Immunol 1990; 145: 1015–1022
  • Cram D S, Fisicaro N, Coppel R L, Whittingham S, Harrison L C. Mapping of multiple B cell epitopes on the 70-kilodalton autoantigen of the U1 ribonucleoprotein complex. J Immunol 1990; 145: 630–635
  • Elkon K B, Hines J J, Chu J, Parnassa A. Epitope mapping of recombinant HeLa Sm B and B' peptides obtained by the poly-merase chain reaction. J Immunol 1990; 145: 636–643
  • Scofield R H, Harley J B. Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus. Proc Natl Acad Sci USA 1991; 88: 3343–3347
  • Reichlin M. Amino acid substitution and the antigenicity of globular proteins. Adv Immunol 1975; 20: 71–123
  • Itoh Y, Itoh K, Frank W B, Reichlin M. Autoantibodies to the Ro/SSA antigen are conformation dependent II: Antibodies to the denatured form of 52 kD Ro/SSA are cross-reactive with a subset of antibodies to the native 60 kD Ro/SSA molecule. Autoimmunity 1992; 00: 000–000

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.