Expression of Recombinant Human Thyroid Peroxidase by the Baculovirus System and Its Use in Elisa Screening for Diagnosis of Autoimmune Thyroid Disease

References

• Banga J P, Barnett P S, Mahadevan D, McGregor A M. Immune recognition of antigen and its relevance to autoimmune desease: Recent advances at the molecular level. Eur J Clin Invest 1988; 19: 107–116
   Google Scholar
• Dumont J E, Vassart G, Refetoff S. The metabolic basis of inherited diseases. Thyroid Disorders, C R Scriver, A L Beaudet, W S Sly, D Valle. McGraw-Hill, New York 1989; 1843–1879
   Google Scholar
• Banga J P, Barnett P S, McGregor A M. Immunological and molecular characteristics of the thyroid peroxidase auto-antigen. Autoimmunity 1991; 8: 335–343
   PubMed Web of Science ®Google Scholar
• McLachlan S M, Rapoport B. The molecular biology of thyroid peroxidase: cloning, expression and role as auto-antigen in autoimmune thyroid disease. Endocrine Rev 1992; 13: 192–206
   PubMed Web of Science ®Google Scholar
• Strakvosch C R, Wenzel B E, Row V V, Volpe R. Immunology of autoimmune thyroid diseases. N Engl J Med 1982; 307: 1499–1507
   PubMedGoogle Scholar
• Czarnocka B, Ruf J, Ferrand M, Carayon P, Lissitzky S. Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid disease. FEBS Letters 1985; 190: 147–152
   PubMed Web of Science ®Google Scholar
• Kotani T, Umeki K, Matsunga S, Kato E, Ohtaki S. Detection of autoantibodies to thyroid peroxidase in autoimmune thyroid diseases by micro-ELISA and immunoblotting. J Clin Endocrinol Metab 1986; 62: 928–933
   PubMed Web of Science ®Google Scholar
• Mariotti S, Anelli S, Ruf J, Bechi R, Czarnocka B, Lombardi A, Carayon P, Pinchera A. Comparison of serum thyroid microsomal and thyroid peroxidase autoantibodies in thyroid disease. J Clin Endocrinol Metab 1987; 65: 987–993
   PubMed Web of Science ®Google Scholar
• Okamoto Y, Hamada N, Saito H, Ohno M, Noh J, Ito K, Morii H. Thyroid peroxidase activity-inhibiting immunoglobulins in patients with autoimmune thyroid disease. J Clin Endocrinol Metabol 1988; 68: 730–734
   Google Scholar
• Portmann L, Fitch F W, Havran W, Hamada N, Franklin W A, DeGroot L. Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies. J Clin Invest 1988; 81: 1217–1224
   PubMedGoogle Scholar
• Magnusson R P, Chazenbalk G D, Gsetautas J, Seto P, Filetti S, DeGroot L J, Rapoport B. Molecular cloning of the complementary deoxyribonucleic acid for human thyroid peroxidase. Mol Endocrinol 1987; 1: 856–861
   PubMedGoogle Scholar
• Kimura S, Kotani T, McBride O W, Umeki K, Hirai K, Nakayama T, Othaki S. Human thyroid peroxidase: Complete cDNA and protein sequence, chromosome mapping, and identification of two alternately spliced mRNAs. Proc Natl Acad Sci USA 1987; 84: 5555–5559
   PubMed Web of Science ®Google Scholar
• Libert F, Ruel J, Ludgate M, Swillens S, Alexander N, Vassart G, Dinsart C. Complete nucleotide sequence of the human thyroperoxidase-microsomal antigen cDNA. Nucleic Acid Res 1987; 15: 6735
   PubMed Web of Science ®Google Scholar
• Kimura S, Kotani T, Ohtaki S, Aoyama T. cDNA-directed expression of human thyroid peroxidase. FEBS Letters 1989; 250: 377–380
   Google Scholar
• Hata J, Yamashita S, Yagihashi S, Kato H, Kabeno S, Hirai K, Kuma K, Kimura S, Umeki K, Kotani T. Stable high level expression of human thyroid peroxidase in cultured Chinese hamster ovary cells. Biochem Biophys Res Commun 1989; 164: 1268–127
   PubMed Web of Science ®Google Scholar
• Kaufman K D, Filetti S, Seto P, Rapoport B. Recombinant human thyroid peroxidase generated in eukaryotic cells: A source of specific antigen for the immunological assay of antimicrosomal antibodies in the sera of patients with autoimmune thyroid disease. J Clin Endocrinol Metab 1990; 70: 724–728
   Google Scholar
• Kendler D L, Martin A, Magnusson R P, Davies T F. Detection of autoantibodies to recombinant human thyroid peroxidase by sensitive enzyme immunoassay. Clin Endocrinol 1990; 33: 751–760
   PubMedGoogle Scholar
• Banga J P, Barnett P S, Ewins D L, Page M J, McGregor A M. Mapping of autoantigenic epitopes on recombinant thyroid peroxidase fragments using the polymerase chain reaction. Autoimmunity 1990; 6: 257–268
   PubMed Web of Science ®Google Scholar
• Elisei R, Mariotti S, Swillens S, Vassart G, Ludgate M. Studies with recombinant autoepitopes of thyroid peroxidase: Evidence suggesting an epitope shared between the thyroid and the gastric parietal cell. Autoimmunity 1990; 8: 65–70
   PubMed Web of Science ®Google Scholar
• Libert F, Ludgate M, Dinsart C, Vassart G. Thyroid-peroxidase, but not the thyrotropin receptor, contains sequential epitopes recognized by autoantibodies in recombinant peptides expressed in the pUEX vector. J Clin Endocrinol Metab 1991; 73: 857–860
   PubMed Web of Science ®Google Scholar
• Ewins D L, Barnett P S, Tomlinson R WS, McGregor A M, Banga J P. Mapping epitope specificities of monoclonal antibodies to thyroid peroxidase using recombinant antigen preparations. Autoimmunity 1992; 11: 141–149
   PubMed Web of Science ®Google Scholar
• Frorath B, Abney C C, Scanarini M, Berthold H, Hunt N, Northemann W. Mapping of a linear autoantigenic epitope within the human thyroid peroxidase using recombinant DNA techniques. J Biochem 1992; 111: 633–637
   Google Scholar
• Maastricht J, Koenig R J, Kaplan M M, Arscott P, Thompson N, Baker J R. Identification of localized autoantibody epitopes in thyroid peroxidase. J Clin Endocrinol Metab 1992; 75: 121–126
   PubMedGoogle Scholar
• Berthold H, Scanarini M, Abney C C, Frorath B, Northemann W. Purification of recombinant antigenic epitopes of the human 68-kDa (Ul) ribonucleoprotein antigen using the expression system pH6EX3 followed by metal chelating affinity chromatography. Prot Exp Purif 1992; 3: 50–56
   PubMedGoogle Scholar
• Porath J. Immobilized metal ion affinity chromatography. Prot Exp Purif 1992; 3: 263–281
   PubMed Web of Science ®Google Scholar
• Luckow V A, Summers M D. Trends in the development of baculovirus expression vectors. BioTechnology 1988; 6: 547–551
   Google Scholar
• Maiorella B, Inlow D, Shauger A, Harano D. Large-scale insect cell-culture for recombinant protein production. Bio Technology 1988; 6: 1406–1410
   Google Scholar
• Kitts P A, Ayres D A, Possee R D. Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucl Acid Res 1990; 18: 5667–5672
   PubMedGoogle Scholar
• O'Reilly D R, Miller L K, Luckow V A. Baculovirus expression vectors. Freeman and Company, New York 1992
   Google Scholar
• Van Dyke M W, Sirito M, Sawadogo M. Single-step purification of bacterially expressed polypeptide containing anoligo-histidine domain. Gene 1992; 111: 99–104
   PubMedGoogle Scholar
• Gustafsson J, Mendel-Hartvig I, Tötterman T H, Karlsson F A. Purification of human thyroid peroxidase using ion exchange liquid chromatography. J Clin Lab Immunol 1987; 23: 57–61
   PubMedGoogle Scholar
• Finke R, Seto P, Rapoport B. Evidence for the highly conformational nature of the epitope(s) on human thyroid peroxidase that are recognized by sera from patients with Hashimoto's thyroiditis. J Clin Endocrinol Metab 1990; 71: 53–59
   PubMed Web of Science ®Google Scholar
• Feldt-Rasmussen U, Hoeier-Madsen M, Bech K, Blichert-Topft M, Bliddal H, Date J, Danneskiold-Samsoe B, Hegedüs L, Hippe E, Hornnes P. J, Kriegbaum N. J, Müller M, Perrild H, Rasmussen N. G, Rasmusson B, Schouboe A. Anti-tyroid peroxidase antibodies in thyroid disorders and non-thyroid autoimmune diseases. Autoimmunity 1991; 9: 245–253
   PubMedGoogle Scholar
• Foti D, Kaufman K D, Chazenbalk G D, Rapoport B. Generation of a biologically active, secreted form of human thyroid peroxidase by site-directed mutagenesis. Mol Endocrinol 1990; 4: 786–791
   PubMedGoogle Scholar
• Gardas A, Domek H, Czarnocka B. The effect of dithiothreitol on thyroid peroxidase and microsomal antigen recognized by auto and monoclonal antibodies. Autoimmunity 1990; 7: 149–156
   PubMed Web of Science ®Google Scholar
• Giraud A, Franc J L, Long Y, Ruf J. Effects of deglycosylation of human thyroperoxidase on its enzymatic and immuno-reactivity. J Endocrinol 1992; 132: 317–323
   Google Scholar
• Luckow V. A. Cloning and expression of heterologous genes in insect cells with baculovirus vectors. Recombinant DNA Technology and Application, A Prokop, R K Pajpai, C Ho. McGraw-Hill, Inc., New York 1991; 97–152
   Google Scholar
• Foti D, Rapoport B. Carbohydrate moieties in recombinant human thyroid peroxidase: Role in recognition by antithyroid peroxidase antibodies in Hashimoto's thyroiditis. Endocrinol 1990; 126: 2983–2988
   PubMedGoogle Scholar
• Ohtaki S, Kotani T, Nakamura Y. Characterization of human thyroid peroxidase purified by monoclonal antibody-assisted chromatography. J Clin Endocrinol Metab 1986; 63: 570–576
   PubMed Web of Science ®Google Scholar
• Zanelli E, Henry M, Charvet B, Malthiery Y. Evidence for an alternate splicing in the thyroperoxidase messenger from patients with graves' disease. Biochem Biophys Res Commun 1990; 170: 735–741
   PubMedGoogle Scholar
• Ruf J, Czarnocka B, Ferrand M, Doullais F, Carayon P. Novel routine assay of thyroperoxidase autoantibodies. Clin Chem 1988; 34: 2231–2234
   PubMed Web of Science ®Google Scholar
• Beever K, Bradbury J, Phillips D, McLaughlin S, Pegg C, Goral A, Overbeck W, Feifel G, Rees Smith B. Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase. Clin Chem 1989; 35: 1949–1954
   PubMed Web of Science ®Google Scholar