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Original Article

Identification of Thyroid Blocking Antibodies and Receptor Epitopes in Autoimmune Hypothyroidism by Affinity Purification Using Synthetic TSH Receptor Peptides

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Pages 69-79 | Received 12 Oct 1995, Published online: 07 Jul 2009

References

  • Braverman L. E., Utiger R. D. Introduction to hypothyroidism. The Thyroid: A Fundamental and Clinical Text. 6th ed, L. E. Braverman, R. D. Utiger. J.B. Lippincott Company., Philadelphia 1991; 919–920
  • Fisher D. A. Neonatal thyroid disease in the offspring of women with autoimmune thyroid disease. Thyroid Today 1986; 9: 1–7
  • Drexhage H. A., Bottazzo G. F., Bitensky L., Chayen J., Doniach D. Thyroid growth-blocking antibodies in primary myxoedema. Nature 1981; 289: 594–596
  • Chiovato L., Vitti P., Santini F., Lopez G., Mammoli C., Bassi P., et al. Incidence of antibodies blocking thyrotropin effect in vitro in patients with euthyroid or hypothyroid autoimmune thyroiditis. J. Clin. Endocrinol. Metab. 1990; 71: 40–45
  • Tokuda Y., Kasagi K., Iida Y., Hatabu H., Misaki T., Arai K., et al. Inhibition of thyrotropin-stimulated iodide uptake in FRTL-5 thyroid cells by crude immunoglobulin fractions from patients with goitrous and atrophic autoimmune thyroiditis. J. Clin. Endocrinol. Metab. 1988; 67: 251–258
  • Kraiem Z., Lahat N., Glaser B., Baron E., Sadeh O., Sheinfeld M. Thyrotropin receptor blocking antibodies: incidence, characterization and in vitro synthesis. Clinical Endocrinology 1987; 27: 409–421
  • Arikawa K., Ichikawa Y., Yoshida T., Shinozawa T., Homma M., Momotani N., et al. Blocking type antithyrotropin receptor antibody in patients with nongoitrous hypothyroidism: its incidence and characteristic of action. J. Clin. Endocrinol. Metab. 1985; 60: 953–959
  • Endo K., Kasagi K., Konishi J., Ikekubo K., Okuno T., Takeda Y., et al. Detection and properties of TSH-binding inhibitor immunoglobulin in patients with Graves' disease and Hashimoto's thyroiditis. J. Clin. Endocrinol. Metab. 1978; 46: 734–739
  • Kohn L. D., Ban T., Okajima F., Shimura H., Shimura Y., Hidaka A., . Cloning and regulation of glycoprotein hormone receptor genes. Molecular Endocrinology: Basic Concepts and Clinical Correlations, B. D. Weintraub, et al. Raven Press, New York 1995; 133–153
  • Chiovato L., Vitti P., Lombardi A., Lopez B., Sanitni F., Macchia E., et al. Detection and characterization of autoantibodies blocking the TSH-dependent cAMP production using FRTL-5 cells. J. Endocrinol. Invest 1987; 10: 383–388
  • Konishi J., Iida Y., Endo K., Misaki T., Nohara Y., Matsuura N., et al. Inhibition of thyrotropin-induced adenosine 3‘5’-monophosphate increase by immunoglobulins from patients with primary myxedema. J. Clin. Endocrinol. Metab. 1983; 57: 544–549
  • Takasu N., Yamada T., Katakura M., Yemauchi K., Shimizu Y., Ishizuki Y. Evidence for thyrotropin (TSH)-blocking activity in goitrous Hashimoto's thyroiditis with assays measuring inhibition of TSH receptor binding and TSH-stimulated thyroid adenosine 3′,5′-monophosphate responses/cell growth by immunoglobulins. J. Clin. Endocrinol. Metab. 1987; 64: 239–245
  • Iida Y., Konishi J., Kasagi K., Misaki T., Arai K., Tokuda Y., et al. Inhibition of thyrotropin-induced growth of rat thyroid cells, FRTL-5, by immunoglobulin G from patients with primary myxedema. J. Clin. Endocrinol. Metab. 1987; 64: 124–130
  • Libert F., Lefort A., Gerard C., Parmentier M., Perret J., Ludgate M., et al. Cloning, sequencing and expression of the human thytrotropin (TSH) receptor: Evidence for binding of autoantibodies. Biochem. Res. Commun. 1989; 165: 1250–1255
  • Nagayama Y., Kaufman K. D., Seto P., Rapoport B. Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor. Biochem. Biophys. Res. Commun. 1989; 165: 1184–1190
  • Misrahi M., Loosfelt H., Atger M., Sar S., Guiochon-Mantel A., Milgrom E. Cloning, sequencing and expression of human TSH receptor. Biochem. Biophys. Res. Commun. 1990; 166: 394–403
  • Morris J. C., Bergert E. R., McCornick D. J. Structure-function studies of the human thyrotropin receptor: Inhibition of binding of labeled thyrotropin (TSH) by synthetic human TSH receptor peptides. J. Biol. Chem. 1993; 268(10)900–10; 905
  • Nagayama Y., Nagataki S. The thyrotropin receptor: its gene expression and structure-function relationships. Thyroid Today 1994; 17: 1–8
  • Kosugi S., Ban T., Akamizu T., Kohn L. D. Site-directed mutagenesis of a portion of the extracellular domain of the rat thyrotropin receptor important in autoimmune thyroid disease and nonhomologous with gonadotropin receptors. J. Biol. Chem. 1991; 266(19)413–19; 418
  • Tahara K., Ban T., Minegishi T., Kohn L. D. Immunoglobulins from Graves' disease patients interact with different sites on TSH receptor/LH-CG chimeras that either TSH or immunoglobulins from idiopathic myxedema patients. Biochem. Biophys. Res. Commun. 1991; 179: 70–77
  • Morris J. C., Gibson J. L., Haas E. J., Bergert E. R., Dallas J. S., Prabhakar B. S. Identification of epitopes and affinity purification of thyroid stimulating auto-antibodies using synthetic human TSH receptor peptides. Autoimmunity 1994; 17: 287–299
  • Volpe R. Autoimmune thyroiditis. The Thyroid: A Fundamental and Clinical Text. 6 edn, L. E. Braverman, R. D. Utiger. J.B. Lippincott Company, Philadelphia 1991; 921–933
  • Morris J. C., Hay I. D., Nelson R. E., Jiang N.-S. Clinical utility of thyrotropin-receptor antibody assays: Comparison of radioreceptor and bioassay methods. Mayo Clin. Proc. 1988; 63: 707–717
  • Kosugi S., Ban T., Akamizu T., Kohn L. D. Identification of separate determinants on the thyrotropin receptor reactive with Graves' thyroid-stimulating antibodies and with thyroid-stimulating blocking antibodies in idiopathic myxedema: These determinants have no homologous sequence on gonadotropin receptors. Mol. Endocrinol. 1992; 6: 168–180
  • Kosugi S., Ban T., Akamizu T., Kohn L. D. Further characterization of a high affinity thyrotropin binding site on the rat thyrotropin receptor which is an epitope for blocking antibodies from idiopathic myxedema patients but not for thyroid stimulating antibodies from Graves' patients. Biochem. Biophys. Res. Commun. 1991; 180: 1118–1124
  • Kosugi S., Ban T., Akamizu T., Valente W., Kohn L. D. Use of thyrotropin receptor (TSHR) mutants to detect stimulating TSHR antibodies in hypothyroid patients with idiopathic myxedema, who have blocking TSHR antibodies. J. Clin. Endocrinol. Metab. 1993; 77: 19–24
  • Ohmori M., Endo T., Ikeda M., Onaya T. Role of N-terminal region of the thyrotropin (TSH) receptor in signal transduction for TSH or thyroid stimulating antibody. Biochem. Biophys. Res. Commun. 1991; 178: 733–738
  • Murakami M., Mori M. Identification of immunogenic regions in human thyrotropin receptor for immunoglobulin G of patients with Graves' disease. Biochem. Biophys. Res. Commun. 1990; 171: 512–518
  • Wadsworth H. L., Chazenbalk G. D., Nagayama Y., Russo D., Rapoport B. AN insertion in the human thyrotropin receptor critical for high affinity hormone binding. Science 1990; 249: 1423–1425

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