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Research Article

Cell-based assays for IGF-I bioactivity measurement: overview, limitations and current trends

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Pages 130-138 | Received 06 May 2014, Accepted 19 Jun 2014, Published online: 25 Jul 2014

References

  • Accornero P, Miretti S, Bersani F, Quaglino E, Martignani E, Baratta M. 2012. Met receptor acts uniquely for survival and morphogenesis of EGFR-dependent normal mammary epithelial and cancer cells. PLoS One 7:e44982
  • Bäck K, Islam R, Johansson G, Chisalita S, Arnqvist H. 2012. Insulin and IGF-I receptors in human cardiac microvascular endothelial cells: Metabolic, mitogenic and anti-inflammatory effects. J Endocrinol 215:89–96
  • Bahmani P, Schellenberger E, Klohs J, Steinbrink J, Cordell R, Zille M, Müller J, et al. 2011. Visualization of cell death in mice with focal cerebral ischemia using fluorescent annexin A5, propidium iodide, and TUNEL staining. J Cereb Blood Flow Metab 31:1311–1320
  • Banerjee P, Bhunia AK. 2009. Mammalian cell-based biosensors for pathogens and toxins. Trends Biotechnol 27:179–188
  • Banerjee P, Franz B, Bhunia AK. 2010. Mammalian cell-based sensor system. Whole Cell Sensing Systems I. Springer. p 21–55
  • Bazhulina N, Nikitin A, Rodin S, Surovaya A, Kravatsky YV, Pismensky V, Archipova V, et al. 2009. Binding of Hoechst 33258 and its derivatives to DNA. J Biomol Struct Dyn 26:701–718
  • Berridge M, Herst P, Tan A. 2005. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol Annu Rev 11:127–152
  • Bidlingmaier M. 2009. Pitfalls of insulin-like growth factor I assays. Horm Res 71:30–33
  • Blanquart C, Karouri S-E, Issad T. 2010. Protein tyrosine phosphatase-1B and T-cell protein tyrosine phosphatase regulate IGF-2-induced MCF-7 cell migration. Biochem Biophys Res Commun 392:83–88
  • Borra RC, Lotufo MA, Gagioti SM, Barros FDM, Andrade PM. 2009. A simple method to measure cell viability in proliferation and cytotoxicity assays. Braz Oral Res 23:255–262
  • Brugts MP, Ranke MB, Hofland LJ, van der Wansem K, Weber K, Frystyk J, Lamberts SW, Janssen JA. 2008. Normal values of circulating insulin-like growth factor-I bioactivity in the healthy population: Comparison with five widely used IGF-I immunoassays. J Clin Endocrinol Metab 93:2539–2545
  • Brugts M, Luermans J, Lentjes E, Van Trooyen-van Vrouwerff N, Van der Horst F, Slee P, Lamberts S, Janssen J. 2009. Heterophilic antibodies may be a cause of falsely low total IGF1 levels. Eur J Endocrinol 161:561–565
  • Burns C, Rigsby P, Moore M, Rafferty B. 2009. The first International Standard for Insulin-like Growth Factor-1 (IGF-1) for immunoassay: Preparation and calibration in an international collaborative study. Growth Horm IGF Res 19:457–462
  • Chen J, Ledet T, Ørskov H, Jessen N, Lund S, Whittaker J, De Meyts P, et al. 2003. A highly sensitive and specific assay for the determination of IGF-I bioactivity in human serum. Am J Physiol Endocrinol Metab 284:E1149–E1155
  • Chen-An P, Andreassen KV, Henriksen K, Li Y, Karsdal MA, Bay-Jensen A-C. 2012. The inhibitory effect of salmon calcitonin on tri-iodothyronine induction of early hypertrophy in articular cartilage. PLoS One 7:e40081
  • Chestnut RE, Quarmby V. 2002. Evaluation of total IGF-I assay methods using samples from Type I and Type II diabetic patients. J Immunol Methods 259:11–24
  • Clemmons DR. 2005. Quantitative measurement of IGF-I and its use in diagnosing and monitoring treatment of disorders of growth hormone secretion. Endo Dev 9:55–65
  • Clemmons D. 2007. Modifying IGF1 activity: An approach to treat endocrine disorders, atherosclerosis and cancer. Nat Rev Drug Discov 6:821–833
  • Clemmons D. 2011. Consensus statement on the standardization and evaluation of growth hormone and insulin-like growth factor assays. Clin Chem 57:555–559
  • Clonetch Laboratories I. 1995. Abacus™ Cell Proliferation Kit Technical Sheet. PT1700-1 PR54432 1–14
  • Connolly DT, Knight MB, Harakas NK, Wittwer AJ, Feder J. 1986. Determination of the number of endothelial cells in culture using an acid phosphatase assay. Anal Biochem 152:136–140
  • Corre J, Mahtouk K, Attal M, Gadelorge M, Huynh A, Fleury-Cappellesso S, Danho C, et al. 2007. Bone marrow mesenchymal stem cells are abnormal in multiple myeloma. Leukemia 21:1079–1088
  • Daughaday WH, Salmon WD Jr, Alexander F. 1959. Sulfation factor activity of sera from patients with pituitary disorders. J Clin Endocrinol Metab 19:743–758
  • Daughaday WH, Hall K, Raben M, Salmon W, Van Den Brande J, Van Wyk J. 1972. Somatomedin: Proposed designation for sulphation factor. Nature 235:107
  • Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W. 2000. Genetically engineered whole-cell sensing systems: Coupling biological recognition with reporter genes. Chem Rev 100:2705–2738
  • Denley A, Cosgrove L, Booker G, Wallace J, Forbes B. 2005. Molecular interactions of the IGF system. Cytokine Growth Factor Rev 16:421–439
  • Duan C, Ren H, Gao S. 2010. Insulin-like growth factors (IGFs), IGF receptors, and IGF-binding proteins: Roles in skeletal muscle growth and differentiation. Gen Comp Endocrin 167:344–345
  • Duffy D, Garrett S, Ellis S, Scott T. 2008. Influence of supramammary lymph node extract on in vitro cell proliferation. Cell Prolif 41:299–309
  • Erb R, Ehlers M. 1950. Resazurin reducing time as an indicator of bovine semen fertilizing capacity. J Dairy Sci 33:853–864
  • Fahnert B, Lilie H, Neubauer P. 2004. Inclusion bodies: Formation and utilisation. Adv Biochem Eng Biotechnol 89:93–142
  • Fields R, Lancaster M. 1993. Dual-attribute continuous monitoring of cell proliferation/cytotoxicity. Am Biotechnol Lab 11:48–50
  • Frankfurt O. 1980. Flow cytometric analysis of double-stranded RNA content distributions. J Histochem Cytochem 28:663–669
  • Fries J. 2009. LONG®R3IGF-I: An insulin alternative exclusively for cell culture applications. BioFiles 5:14–18
  • Froesch E, Bürgi H, Ramseier E, Bally P, Labhart A. 1963. Antibody-suppressible and nonsuppressible insulin-like activities in human serum and their physiologic significance. An insulin assay with adipose tissue of increased precision and specificity. J Clin Invest 42:1816–1834
  • Frystyk J, Brick D, Gerweck A, Utz A, Miller K. 2009. Bioactive insulin-like growth factor-I in obesity. J Clin Endocrinol Metab 94:3093–3097
  • Frystyk J, Freda P, Clemmons D. 2010. The current status of IGF-I assays – A 2009 update. Growth Horm IGF Res 20:8–18
  • Frystyk J, Schou AJ, Heuck C, Vorum H, Lyngholm M, Flyvbjerg A, Wolthers OD. 2013. Prednisolone reduces the ability of serum to activate the IGF1 receptor in vitro without affecting circulating total or free IGF1. Eur J Endocrinol 168:1–8
  • Guler H, Schmid C, Zapf J, Froesch E. 1990. Effects of insulin-like growth factor I in man. Acta Paediatr 79:52–54
  • Hedman CA, Frystyk J, Lindström T, Oskarsson P, Arnqvist HJ. 2013. Intraperitoneal insulin delivery to patients with type 1 diabetes results in higher serum IGF-I bioactivity than continuous subcutaneous insulin infusion. Clin Endocrinol 1:1365–2265
  • Héron-Milhavet L, Karas M, Goldsmith CM, Baum BJ, LeRoith D. 2001. Insulin-like growth factor-I (IGF-I) receptor activation rescues UV-damaged cells through a p38 signaling pathway potential role of the IGF-I receptor in DNA repair. J Biol Chem 276:18185–18192
  • Ho L, Yemul S, Knable L, Katsel P, Zhao R, Haroutunian V, Pasinetti GM. 2012. Insulin receptor expression and activity in the brains of nondiabetic sporadic Alzheimer’s disease cases. Int J Alzheimer’s Dis 2012:321280
  • Hou X, Huang F, Macedo L, Harrington S, Reeves K, Greer A, Finckenstein F, et al. 2011. The dual IGF-1R/InsR inhibitor, BMS-754807, demonstrates synergy in combination with hormonal agents in vitro and enhances in vivo activity in an estrogen-dependent breast cancer model. Can Res 71:7597–7607
  • Hsu S, Bollag WB, Lewis J, Huang Q, Singh B, Sharawy M, Yamamoto T, Schuster G. 2003. Green tea polyphenols induce differentiation and proliferation in epidermal keratinocytes. J Pharmacol Exp Ther 306:29–34
  • Huhtala T, Rytkönen J, Jalanko A, Kaasalainen M, Salonen J, Riikonen R, Närvänen A. 2012. Native and complexed IGF-1: Biodistribution and pharmacokinetics in infantile neuronal ceroid lipofuscinosis. J Drug Deliv 2012:626417(1–8)
  • Irie HY, Shrestha Y, Selfors LM, Frye F, Iida N, Wang Z, Zou L, et al. 2010. PTK6 regulates IGF-1-induced anchorage-independent survival. PLoS One 5:e11729
  • Jiratchariyakul W, Kummalue T. 2011. Experimental therapeutics in breast cancer cells. In: Gunduz E, Editor. Breast cancer – Current and alternative therapeutic modalities. Rijeka, Croatia: InTech
  • Jones L, Gray M, Yue S, Haugland R, Singer V. 2001. Sensitive determination of cell number using the CyQUANT cell proliferation assay. J Immunol Methods 254:85–94
  • Kanwal S, Fardini Y, Pagesy P, N'Tumba-Byn T, Pierre-Eugène C, Masson E, Hampe C, Issad T. 2013. O-GlcNAcylation-inducing treatments inhibit estrogen receptor? Expression and confer resistance to 4-OH-tamoxifen in human breast cancer-derived MCF-7 cells. PLoS One 7:e69150
  • Khosravi MJ, Diamandi A, Mistry J, Lee PD. 1996. Noncompetitive ELISA for human serum insulin-like growth factor-I. Clin Chem 42:1147–1154
  • Latchney SE, Lioy DT, Henry EC, Gasiewicz TA, Strathmann FG, Mayer-Pröschel M, Opanashuk LA. 2010. Neural precursor cell proliferation is disrupted through activation of the aryl hydrocarbon receptor by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin. Stem Cells Dev 20:313–326
  • Li Y, Geng Y-J. 2010. A potential role for insulin-like growth factor signaling in induction of pluripotent stem cell formation. Growth Horm IGF Res 20:391–398
  • Livingstone C. 2013. Insulin-like growth factor-I (IGF-I) and clinical nutrition. Clin Sci 125:265–280
  • Maghni K, Nicolescu O, Martin J. 1999. Suitability of cell metabolic colorimetric assays for assessment of CD4+ T cell proliferation: Comparison to 5-bromo-2-deoxyuridine (BrdU) ELISA. J Immunol Methods 223:185–194
  • Mairet-Coello G, Tury A, DiCicco-Bloom E. 2009. IGF-1 promotes G1/S cell cycle progression through bidirectional regulation of cyclins and CDK inhibitors via the PI3K/Akt pathway in developing rat cerebral cortex. J Neurosci 29:775–788
  • Martino MM, Hubbell JA. 2010. The 12th–14th type III repeats of fibronectin function as a highly promiscuous growth factor-binding domain. FASEB J 24:4711–4721
  • Moghim S, Mofid M, Rahaie M, Fallah J, Jafari S, Babaeipour V. 2012. Cloning and over-expression of recombinant human insulin-like growth factor-I in E. coli. Res Pharm Sci 7:S417
  • Morris SM. 1991. The genetic toxicology of 5-bromodeoxyuridine in mammalian cells. Mutat Res Rev Genet Toxicol 258:161–188
  • Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63
  • Muppidi JR, Arnon TI, Bronevetsky Y, Veerapen N, Tanaka M, Besra GS, Cyster JG. 2011. Cannabinoid receptor 2 positions and retains marginal zone B cells within the splenic marginal zone. J Exp Med 208:1941–1948
  • Musiychuk K, Sivalenka R, Jaje J, Bi H, Flores R, Shaw B, Jones RM, et al. 2013. Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitro. Stem Cell Dev 22:2326–2340
  • O’Brien J, Wilson I, Orton T, Pognan F. 2000. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem 267:5421–5426
  • Parandoosh Z, Bogowitz CA, Nova MP. 1998. A fluorometric assay for the measurement of endothelial cell density in vitro. In Vitro Cell Dev Biol Anim 34:772–776
  • Patterson SS, Dionisi HM, Gupta RK, Sayler GS. 2005. Codon optimization of bacterial luciferase (lux) for expression in mammalian cells. J Ind Microbiol Biotechnol 32:115–123
  • Petrenko Y, Gorokhova N, Tkachova E, Petrenko AY. 2005. The reduction of Alamar Blue by peripheral blood lymphocytes and isolated mitochondria. Ukr Biokhim Zh 77:100–105
  • Pickard A, Cichon A-C, Menges C, Patel D, McCance DJ. 2012. Regulation of epithelial differentiation and proliferation by the stroma: A role for the retinoblastoma protein. J Invest Dermatol 132:2691–2699
  • Piroozmand S, Miroliaei M, Atyabi S, Kaghazian H, Moshtagheian J, Irani S, Norouzeyan D. 2013. Effect of insulin-like growth factor-I on apoptosis in Chinese hamster ovary cells for biopharmaceutical production. Tehran Univ Med J 22:251–258
  • Plumb JA. 2004. Cell sensitivity assays: The MTT assay. Cancer Cell Culture. Totowa, NJ: Springer. p 165–169
  • Plumb JA. 2004. Cell sensitivity assays: The MTT assay. Methods Mol Med 88:165–169
  • Promega. CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. Technical Bulletin. Vol TB169. USA 2009:1–16
  • Purschke M, Rubio N, Held KD, Redmond RW. 2010. Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy. Photochem Photobiol Sci 9:1634–1639
  • Rampersad S. 2012. Multiple applications of alamar blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors 12:12347–12360
  • Ranke MB, Feldt-Rasmussen U, Bang P, Baxter RC, Camacho-Hubner C, Clemmons DR, Juul A, et al. 2001. How should insulin-like growth factor I be measured? A consensus statement. Horm Res 55:106–109
  • Rinderknecht E, Humbel RE. 1978. Primary structure of human insulin-like growth factor II. FEBS Lett 89:283–286
  • Roda A, Pasini P, Mirasoli M, Michelini E, Guardigli M. 2004. Biotechnological applications of bioluminescence and chemiluminescence. Trends Biotechnol 22:295–303
  • Roozbeh A, Abramov A, Diao C, Kargacin M, Kargacin G, French R, Pavlov E. 2008. High sensitivity, quantitative measurements of polyphosphate using a new DAPI-based approach. J Fluroresc 18:859–866
  • Ross JS, Linette GP, Stec J, Ross MS, Anwar S, Boguniewicz A. 2003. DNA ploidy and cell cycle analysis in breast cancer. Am J Clin Pathol Patt Rev 120:S72–S84
  • Ruscica M, Magni P, Steffani L, Gatto F, Albertelli M, Rametta R, Valenti L, et al. 2014. Characterization and sub-cellular localization of SS1R, SS2R, and SS5R in human late-stage prostate cancer cells: Effect of mono-and bi-specific somatostatin analogs on cell growth. Mol Cell Endocrinol 382:860–870
  • Sadick MD, Intintoli A, Quarmby V, McCoy A, Canova-Davis E, Ling V. 1999. Kinase receptor activation (KIRA): A rapid and accurate alternative to end-point bioassays. J Pharm Biomed Anal 19:883–891
  • Saegusa J, Yamaji S, Ieguchi K, Wu C-Y, Lam KS, Liu F-T, Takada YK, Takada Y. 2009. The direct binding of insulin-like growth factor-1 (IGF-1) to integrin αvβ3 is involved in IGF-1 signaling. J Biol Chem 284:24106–24114
  • Safdie F, Brandhorst S, Wei M, Wang W, Lee C, Hwang S, Conti PS, et al. 2012. Fasting enhances the response of glioma to chemo- and radiotherapy. PLoS One 7:e44603
  • Salmon WD Jr, Daughaday WH. 1975. A hormonally controlled serum factor which stimulates sulfate incorporation by cartilage in vitro. J Lab Clin Med 49:825–836
  • Savage M, Camacho-Hübner C, Dunger D. 2004. Therapeutic applications of the insulin-like growth factors. Growth Horm IGF Res 14:301–308
  • Serra C, Bhasin S, Tangherlini F, Barton ER, Ganno M, Zhang A, Shansky J, et al. 2011. The role of GH and IGF-I in mediating anabolic effects of testosterone on androgen-responsive muscle. Endocrinology 152:193–206
  • Shinohara Y, Tsuchiya S, Hatae K, Honda MJ. 2012. Effect of vitronectin bound to insulin-like growth factor-I and insulin-like growth factor binding protein-3 on porcine enamel organ-derived epithelial cells. Int J Dent 2012:386282
  • Simpson H, Umpleby A, Russell-Jones D. 1998. Insulin-like growth factor-I and diabetes. A review. Growth Horm IGF Res 8:83–95
  • Sivaramakrishnan M, Kashyap AS, Amrein B, Saenger S, Meier S, Staudenmaier C, Upton Z, Metzger F. 2013. PEGylation of lysine residues reduces the pro-migratory activity of IGF-I. Biochim Biophys Acta 1830:4734–4742
  • Siwanowicz I, Popowicz GM, Wisniewska M, Huber R, Kuenkele K-P, Lang K, Engh RA, Holak TA. 2005. Structural basis for the regulation of insulin-like growth factors by IGF binding proteins. Structure 13:155–167
  • Skov J, Frystyk J, Christiansen JS. 2014. GLP-1 infusion reduces IGFBP-1 serum level in humans. Growth Horm IGF Res 24:67–70
  • Slater T, Sawyer B, Sträuli U. 1963. Studies on succinate-tetrazolium reductase systems: III. Points of coupling of four different tetrazolium salts III. Points of coupling of four different tetrazolium salts. Biochim Biophys Acta 77:383–393
  • Støving RK, Chen J-W, Glintborg D, Brixen K, Flyvbjerg A, Hørder K, Frystyk J. 2007. Bioactive insulin-like growth factor (IGF) I and IGF-binding protein-1 in anorexia nervosa. J Clin Endocrinol Metab 92:2323–2329
  • Sylvester PW. 2011. Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability. Totowa, NJ: Springer
  • Tian J, Berton TR, Shirley SH, Lambertz I, Gimenez-Conti IB, DiGiovanni J, Korach KS, et al. 2012. Developmental stage determines estrogen receptor alpha expression and non-genomic mechanisms that control IGF-1 signaling and mammary proliferation in mice. J Clin Invest 122:192–204
  • Uptima I. 2011. UptiBlue™ viable cell counting reagent Technical Sheet. Vol. FT-669412. France. p 1–4
  • Upton Z, Cuttle L, Noble A, Kempf M, Topping G, Malda J, Xie Y, et al. 2008. Vitronectin: Growth factor complexes hold potential as a wound therapy approach. J Investig Dermatol 128:1535–1544
  • Usuki S, Tsai Y, Morikawa K, Nonaka S, Okuhara Y, Kise M, Yu R. 2011. IGF-1 induction by acylated steryl b-glucosides found in a pre-germinated brown rice diet reduces oxidative stress in streptozotocin-induced diabetes. PLoS One 12:1–12
  • Varewijck AJ, Janssen J, Vähätalo M, Hofland L, Lamberts S, Yki-Järvinen H. 2012. Addition of insulin glargine or NPH insulin to metformin monotherapy in poorly controlled type 2 diabetic patients decreases IGF-I bioactivity similarly. Diabetologia 55:1186–1194
  • Ventura S, Villaverde A. 2006. Protein quality in bacterial inclusion bodies. Trends Biotechnol 24:179–185
  • Vitale G, Brugts M, Ogliari G, Castaldi D, Fatti L, Varewijck A, Lamberts S, et al. 2012. Low circulating IGF-I bioactivity is associated with human longevity: Findings in centenarians’ offspring. Aging 4:580–589
  • Volkova E, Robinson BA, Willis J, Currie MJ, Dachs GU. 2014. Marginal effects of glucose, insulin and insulin-like growth factor on chemotherapy response in endothelial and colorectal cancer cells. Oncol Lett 7:311–320
  • Voytik-Harbin SL, Brightman AO, Waisner B, Lamar CH, Badylak SF. 1998. Application and evaluation of the alamarBlue assay for cell growth and survival of fibroblasts. In Vitro Cell Dev-An 34:239–246
  • Wang P, Henning SM, Heber D. 2010. Limitations of MTT and MTS-based assays for measurement of antiproliferative activity of green tea polyphenols. PLoS One 5:e10202
  • Wojtalla A, Salm F, Christiansen DG, Cremona T, Cwiek P, Shalaby T, Gross N, et al. 2012. Novel agents targeting the IGF-1R/PI3K pathway impair cell proliferation and survival in subsets of medulloblastoma and neuroblastoma. PLoS One 7:e47109
  • Wu Y-C, Zhu M, Robertson DM. 2012. Novel nuclear localization and potential function of insulin-like growth factor-1 receptor/insulin receptor hybrid in corneal epithelial cells. PLoS One 7:e42483
  • Xie T, Qiu Q, Zhang W, Ning T, Yang W, Zheng C, Wang C, et al. 2008. A biologically active rhIGF-1 fusion accumulated in transgenic rice seeds can reduce blood glucose in diabetic mice via oral delivery. Peptides 29:1862–1870
  • Yamada H, Iijima K, Tomita O, Taguchi T, Miharu M, Kobayashi K, Okita H, et al. 2013. Effects of insulin-like growth factor-1 on B-cell precursor acute lymphoblastic leukemia. Int J Hematol 97:73–82
  • Yang T-T, Sinai P, Kain SR. 1996. An acid phosphatase assay for quantifying the growth of adherent and nonadherent cells. Anal Biochem 241:103–108

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