Prediction of clonogenic cell survival curves based on the number of residual DNA double strand breaks measured by γH2AX staining

References

• Banath JP, Macphail SH, Olive PL. 2004. Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines. Cancer Research 64:7144–7149.
   PubMed Web of Science ®Google Scholar
• Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S, Pommier Y. 2008. GammaH2AX and cancer. Nature Reviews Cancer 8:957–967.
   PubMed Web of Science ®Google Scholar
• Burma S, Chen BP, Murphy M, Kurimasa A, Chen DJ. 2001. ATM phosphorylates histone H2AX in response to DNA double-strand breaks. Journal of Biological Chemistry 276:42462–42467.
   PubMed Web of Science ®Google Scholar
• Celeste A, Fernandez-Capetillo O, Kruhlak MJ, Pilch DR, Staudt DW, Lee A, Bonner RF, Bonner WM, Nussenzweig A. 2003. Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nature Cell Biology 5:675–679.
   PubMed Web of Science ®Google Scholar
• Downs JA. 2007. Chromatin structure and DNA double-strand break responses in cancer progression and therapy. Oncogene 26:7765–7772.
   PubMed Web of Science ®Google Scholar
• Eicheler W, Zips D, Dorfler A, Grenman R, Baumann M. 2002. Splicing mutations in TP53 in human squamous cell carcinoma lines influence immunohistochemical detection. Journal of Histochemistry and Cytochemistry 50:197–204.
   PubMed Web of Science ®Google Scholar
• Fernandez-Capetillo O, Chen HT, Celeste A, Ward I, Romanienko PJ, Morales JC, Naka K, Xia Z, Camerini-Otero RD, Motoyama N, Carpenter PB, Bonner WM, Chen J, Nussenzweig A. 2002. DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nature Cell Biology 4:993–997.
   PubMed Web of Science ®Google Scholar
• Foster ER, Downs JA. 2005. Histone H2A phosphorylation in DNA double-strand break repair. Federation of European Biochemical Societies Journal, FEBS Journal, (formerly: The European Journal of Biochemistry; EJB) 272:3231–3240.
   Google Scholar
• Girard PM, Riballo E, Begg AC, Waugh A, Jeggo PA. 2002. Nbs1 promotes ATM dependent phosphorylation events including those required for G1/S arrest. Oncogene 21:4191–4199.
   PubMedGoogle Scholar
• Goldberg M, Stucki M, Falck J, D'Amours D, Rahman D, Pappin D, Bartek J, Jackson SP. 2003. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 421:952–956.
   PubMed Web of Science ®Google Scholar
• Goodarzi AA, Noon AT, Deckbar D, Ziv Y, Shiloh Y, Lobrich M, Jeggo PA. 2008. ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin. Molecular Cell 31:167–177.
   PubMed Web of Science ®Google Scholar
• Haber JE. 2000. Partners and pathwaysrepairing a double-strand break. Trends in Genetics 16:259–264.
   PubMed Web of Science ®Google Scholar
• Hoeijmakers JH. 2001. Genome maintenance mechanisms for preventing cancer. Nature 411:366–374.
   PubMed Web of Science ®Google Scholar
• Jackson SP. 2002. Sensing and repairing DNA double-strand breaks. Carcinogenesis 23:687–696.
   PubMed Web of Science ®Google Scholar
• Kang J, Ferguson D, Song H, Bassing C, Eckersdorff M, Alt FW, Xu Y. 2005. Functional interaction of H2AX, NBS1, and p53 in ATM-dependent DNA damage responses and tumor suppression. Molecular and Cellular Biology 25:661–670.
   PubMedGoogle Scholar
• Kasten-Pisula U, Menegakis A, Brammer I, Borgmann K, Mansour WY, Degenhardt S, Krause M, Schreiber A, Dahm-Daphi J, Petersen C, Dikomey E, Baumann M. 2009. The extreme radiosensitivity of the squamous cell carcinoma SKX is due to a defect in double-strand break repair. Radiotherapy Oncology 90:257–264.
   PubMed Web of Science ®Google Scholar
• Keogh MC, Kim JA, Downey M, Fillingham J, Chowdhury D, Harrison JC, Onishi M, Datta N, Galicia S, Emili A, Lieberman J, Shen X, Buratowski S, Haber JE, Durocher D, Greenblatt JF, Krogan NJ. 2006. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 439:497–501.
   PubMed Web of Science ®Google Scholar
• Kinner A, Wu W, Staudt C, Iliakis G. 2008. Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Research 36:5678–5694.
   PubMed Web of Science ®Google Scholar
• Klokov D, MacPhail SM, Banath JP, Byrne JP, Olive PL. 2006. Phosphorylated histone H2AX in relation to cell survival in tumor cells and xenografts exposed to single and fractionated doses of X-rays. Radiotherapy Oncology 80:223–229.
   PubMed Web of Science ®Google Scholar
• MacPhail SH, Banath JP, Yu TY, Chu EH, Lambur H, Olive PL. 2003. Expression of phosphorylated histone H2AX in cultured cell lines following exposure to X-rays. International Journal of Radiation Biology 79:351–358.
   PubMed Web of Science ®Google Scholar
• Mahrhofer H, Burger S, Oppitz U, Flentje M, Djuzenova CS. 2006. Radiation induced DNA damage and damage repair in human tumor and fibroblast cell lines assessed by histone H2AX phosphorylation. International Journal of Radiation Oncology, Biology, Physics 64:573–580.
   PubMed Web of Science ®Google Scholar
• McManus KJ, Hendzel MJ. 2005. ATM-dependent DNA damage-independent mitotic phosphorylation of H2AX in normally growing mammalian cells. Molecular Biology of the Cell 16:5013–5025.
   PubMed Web of Science ®Google Scholar
• Mirzayans R, Severin D, Murray D. 2006. Relationship between DNA double-strand break rejoining and cell survival after exposure to ionizing radiation in human fibroblast strains with differing ATM/p53 status: Implications for evaluation of clinical radiosensitivity. International Journal of Radiation Oncology, Biology, Physics 66:1498–1505.
   PubMedGoogle Scholar
• Morrison AJ, Shen X. 2005. DNA repair in the context of chromatin. Cell Cycle 4:568–571.
   PubMedGoogle Scholar
• Olive PL, Banath JP. 2009. Kinetics of H2AX phosphorylation after exposure to cisplatin. Cytometry Part B Clinical Cytometry 76:79–90.
   PubMedGoogle Scholar
• Olive PL, Banath JP, Sinnott LT. 2004. Phosphorylated histone H2AX in spheroids, tumors, and tissues of mice exposed to etoposide and 3-amino-1,2,4-benzotriazine-1,3-dioxide. Cancer Research 64:5363–5369.
   PubMed Web of Science ®Google Scholar
• Paull TT, Rogakou EP, Yamazaki V, Kirchgessner CU, Gellert M, Bonner WM. 2000. A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Current Biology 10:886–895.
   PubMed Web of Science ®Google Scholar
• Pilch DR, Sedelnikova OA, Redon C, Celeste A, Nussenzweig A, Bonner WM. 2003. Characteristics of gamma-H2AX foci at DNA double-strand breaks sites. Biochemistry and Cell Biology 81:123–129.
   PubMed Web of Science ®Google Scholar
• Qvarnstrom OF, Simonsson M, Johansson KA, Nyman J, Turesson I. 2004. DNA double strand break quantification in skin biopsies. Radiotherapy Oncology 72:311–317.
   PubMed Web of Science ®Google Scholar
• Rappold I, Iwabuchi K, Date T, Chen J. 2001. Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways. Journal of Cell Biology 153:613–620.
   PubMed Web of Science ®Google Scholar
• Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. 1998. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. Journal of Biological Chemistry 273:5858–5868.
   PubMed Web of Science ®Google Scholar
• Rothkamm K, Kruger I, Thompson LH, Lobrich M. 2003. Pathways of DNA double-strand break repair during the mammalian cell cycle. Molecular and Cellular Biology 23:5706–5715.
   PubMed Web of Science ®Google Scholar
• Schultz LB, Chehab NH, Malikzay A, Halazonetis TD. 2000. p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks. Journal of Cell Biology 151:1381–1390.
   PubMed Web of Science ®Google Scholar
• Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM. 2002. Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody. Radiation Research 158:486–492.
   PubMed Web of Science ®Google Scholar
• Soutoglou E, Misteli T. 2008. Activation of the cellular DNA damage response in the absence of DNA lesions. Science 320:1507–1510.
   PubMed Web of Science ®Google Scholar
• Stewart GS, Wang B, Bignell CR, Taylor AM, Elledge SJ. 2003. MDC1 is a mediator of the mammalian DNA damage checkpoint. Nature 421:961–966.
   PubMed Web of Science ®Google Scholar
• Stucki M, Jackson SP. 2006. GammaH2AX and MDC1: Anchoring the DNA-damage-response machinery to broken chromosomes. DNA Repair 5:534–543.
   PubMed Web of Science ®Google Scholar
• Takahashi A, Ohnishi T. 2005. Does gammaH2AX foci formation depend on the presence of DNA double strand breaks? Cancer Letters 229:171–179.
   PubMed Web of Science ®Google Scholar
• Wada S, Van Khoa T, Kobayashi Y, Funayama T, Ogihara K, Ueno S, Ito N. 2005. Prediction of cellular radiosensitivity from DNA damage induced by gamma-rays and carbon ion irradiation in canine tumor cells. Journal of Veterinary Medical Science 67:1089–1095.
   PubMedGoogle Scholar
• Ward IM, Chen J. 2001. Histone H2AX is phosphorylated in an ATR-dependent manner in response to replicational stress. Journal of Biological Chemistry 276:47759–47762.
   PubMed Web of Science ®Google Scholar
• Yoshikawa T, Kashino G, Ono K, Watanabe M. 2009. Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities. Journal of Radiation Research (Tokyo) 50:151–160.
   PubMed Web of Science ®Google Scholar