194
Views
2
CrossRef citations to date
0
Altmetric
AGAROSE MACROCOLONY ASSAY FOR PRIMARY FIBROBLASTS

Agarose overlay selectively improves macrocolony formation and radiosensitivity assessment in primary fibroblasts

, , , , &
Pages 401-406 | Received 08 Oct 2013, Accepted 06 Feb 2014, Published online: 20 Mar 2014

References

  • Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A. 1982. Extracellular matrix proteins of human epidermal keratinocytes and feeder 3t3 cells. J Cell Biol 94:497–505.
  • Baskar R, Balajee AS, Geard CR. 2007. Effects of low and high let radiations on bystander human lung fibroblast cell survival. Int J Radiat Biol 83:551–559.
  • Brock WA, Tucker SL, Geara FB, Turesson I, Wike J, Nyman J, Peters LJ. 1995. Fibroblast radiosensitivity versus acute and late normal skin responses in patients treated for breast cancer. Int J Radiat Oncol Biol Phys 32:1371–1379.
  • Chan RW, Schwab KE, Gargett CE. 2004. Clonogenicity of human endometrial epithelial and stromal cells. Biol Reprod 70:1738–1750.
  • Eastham AM, Marples B, Kiltie AE, Orton CJ, West CM. 1999. Fibroblast radiosensitivity measured using the comet DNA-damage assay correlates with clonogenic survival parameters. Br J Cancer 79:1366–1371.
  • Enninga IC, Groenendijk RT, Filon AR, van Zeeland AA, Simons JW. 1986. The wavelength dependence of u.V.-induced pyrimidine dimer formation, cell killing and mutation induction in human diploid skin fibroblasts. Carcinogenesis 7:1829–1836.
  • Enninga IC, Groenendijk RT, van Zeeland AA, Simons JW. 1985a. Differential response of human fibroblasts to the cytotoxic and mutagenic effects of UV radiation in different phases of the cell cycle. Mutat Res 148:119–128.
  • Enninga IC, Groenendijk RT, van Zeeland AA, Simons JW. 1985b. Recovery of growth-arrested human fibroblasts from UV-induced lethal damage is inhibited by low cell density or sodium butyrate. Mutat Res 152:233–241.
  • Gery B, Coppey J, Little JB. 1996. Modulation of clonogenicity, growth, and radiosensitivity of three human epidermoid tumor cell lines by a fibroblastic environment. Int J Radiat Oncol Biol Phys 34:1061–1071.
  • Grenman R, Burk D, Virolainen E, Buick RN, Church J, Schwartz DR, Carey TE. 1989. Clonogenic cell assay for anchorage-dependent squamous carcinoma cell lines using limiting dilution. Int J Cancer 44:131–136.
  • Grenman R, Burk D, Virolainen E, Wagner JG, Lichter AS, Carey TE. 1988. Radiosensitivity of head and neck cancer cells in vitro. A 96-well plate clonogenic cell assay for squamous cell carcinoma. Arch Otolaryngol Head Neck Surg 114:427–431.
  • Haikonen J, Rantanen V, Pekkola K, Kulmala J, Grenman R. 2003. Does skin fibroblast radiosensitivity predict squamous cancer cell radiosensitivity of the same individual?Int J Cancer 103:784–788.
  • Johansen J, Bentzen SM, Overgaard J, Overgaard M. 1996. Relationship between the in vitro radiosensitivity of skin fibroblasts and the expression of subcutaneous fibrosis, telangiectasia, and skin erythema after radiotherapy. Radiother Oncol 40:101–109.
  • Kiltie AE, Orton CJ, Ryan AJ, Roberts SA, Marples B, Davidson SE, Hunter RD, Margison GP, West CM, Hendry JH. 1997. A correlation between residual DNA double-strand breaks and clonogenic measurements of radiosensitivity in fibroblasts from preradiotherapy cervix cancer patients. Int J Radiat Oncol Biol Phys 39:1137–1144.
  • Moertel H, Georgi JC, Distel L, Eyrich W, Fritsch M, Grabenbauer G, Sauer R. 2004. Effects of low energy protons on clonogenic survival, DSB repair and cell cycle in human glioblastoma cells and b14 fibroblasts. Radiother Oncol 73:S115–118.
  • Ohneseit PA, Wildemann A, Herskind C, Bamberg M, Rodemann HP. 2002. Differential response of tumor cells and normal fibroblasts to fractionated combined treatment with topotecan and ionizing radiation. Int J Radiat Biol 78:125–132.
  • Pomp J, Wike JL, Ouwerkerk IJ, Hoogstraten C, Davelaar J, Schrier PI, Leer JW, Thames HD, Brock WA. 1996. Cell density dependent plating efficiency affects outcome and interpretation of colony forming assays. Radiother Oncol 40:121–125.
  • Puck TT, Marcus PI. 1956. Action of x-rays on mammalian cells. J Exp Med 103:653–666.
  • Sangsuwan T, Haghdoost S. 2008. The nucleotide pool, a target for low-dose gamma-ray-induced oxidative stress. Radiat Res 170:776–783.
  • Simons JW. 1979. Development of a liquid-holding technique for the study of DNA-repair in human diploid fibroblasts. Mutat Res 59:273–283.
  • Slamenova D, Gabelova A, Ruzekova L, Chalupa I, Horvathova E, Farkasova T, Bozsakyova E, Stetina R. 1997. Detection of mnng-induced DNA lesions in mammalian cells; validation of comet assay against DNA unwinding technique, alkaline elution of DNA and chromosomal aberrations. Mutat Res 383:243–252.
  • Vladimirskaia EB, Aimanbetova AM, Kolosova L, Tsuria VM. 1989. The dependence of the clonogenic fibroblast proliferation of human bone marrow on the feeder. Eksp Onkol 11:53–56.
  • Von Hoff DD, Forseth BJ, Huong M, Buchok JB, Lathan B. 1986. Improved plating efficiencies for human tumors cloned in capillary tubes versus petri dishes. Cancer Res 46:4012–4017.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.