References
- Amundson DM, Zhou M. 1999. Fluorometric method for the enzymatic determination of cholesterol. J Biochem Biophys Meth 38:43–52.
- Banerjee P, Joo JB, Buse JT, Dawson G. 1995. Differential solubilization of lipids along with membrane proteins by different classes of detergents. Chem Phys Lipids 77:65–78.
- Björk K, Sjögren B, Svenningsson P. 2010. Regulation of serotonin receptor function in the nervous system by lipid rafts and adaptor proteins. Exp Cell Res 316:1351–1356.
- Brown DA, London E. 1998. Structure and origin of ordered lipid domains in biological membranes. J Membr Biol 164:103–114.
- Brown DA, London E. 2000. Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J Biol Chem 275:17221–17224.
- Brown DA, Rose JK. 1992. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68:533–544.
- Chamberlain LH. 2004. Detergents as tools for the purification and classification of lipid rafts. FEBS Lett 559:1–5.
- Fishman PH. 1982. Role of membrane ganglioides in the binding and action of bacterial toxins. J Membr Biol 69:85–97.
- Ganguly S, Chattopadhyay A. 2010. Cholesterol depletion mimics the effect of cytoskeletal destabilization on membrane dynamics of the serotonin1A receptor: A zFCS study. Biophys J 99:1397–1407.
- Hajduch E, Turban S, Le Liepvre X, Le Lay S, Lipina C, Dimopoulos N, 2008. Targeting of PKCζ and PKB to caveolin-enriched microdomains represents a crucial step underpinning the disruption in PKB-directed signalling by ceramide. Biochem J 410:369–379.
- Hansen GH, Niels-Christiansen L, Thorsen E, Immerdal L, Danielsen EM. 2000. Cholesterol depletion of enterocytes: Effect on the golgi complex and apical membrane trafficking. J Biol Chem 275:5136–5142.
- Heerklotz H. 2002. Triton promotes domain formation in lipid raft mixtures. Biophys J 83:2693–2701.
- Heilker R, Wolff M, Tautermann CS, Bieler M. 2009. G-protein-coupled receptor-focused drug discovery using a target class platform approach. Drug Discov Today 14:231–240.
- Hooper NM. 1999. Detergent-insoluble glycosphingolipid/cholesterol-rich membrane domains, lipid rafts and caveolae. Mol Membr Biol 16:145–156.
- Jacobson K, Mouritsen OG, Anderson RGW. 2007. Lipid rafts: At a crossroad between cell biology and physics. Nat Cell Biol 9:7–14.
- Kalipatnapu S, Chattopadhyay A. 2004. A GFP fluorescence-based approach to determine detergent insolubility of the human serotonin1A receptor. FEBS Lett 576:455–460.
- Kalipatnapu S, Chattopadhyay A. 2005. Membrane organization of the human serotonin1A receptor monitored by detergent insolubility using GFP fluorescence. Mol Membr Biol 22:539–547.
- Kalipatnapu S, Chattopadhyay A. 2007a. Membrane organization of the serotonin1A receptor monitored by a detergent-free approach. Cell Mol Neurobiol 27:463–474.
- Kalipatnapu S, Chattopadhyay A. 2007b. Membrane organization and function of the serotonin1A receptor. Cell Mol Neurobiol 27:1097–1116.
- Kalipatnapu S, Pucadyil TJ, Harikumar KG, Chattopadhyay A. 2004. Ligand binding characteristics of the human serotonin1A receptor heterologously expressed in CHO cells. Biosci Rep 24:101–115.
- Lingwood D, Simons K. 2010. Lipid rafts as a membrane-organizing principle. Science 327:46–50.
- Mayor S, Maxfield FR. 1995. Insolubility and redistribution of GPI-anchored proteins at the cell surface after detergent treatment. Mol Biol Cell 6:929–944.
- McClare CWF. 1971. An accurate and convenient organic phosphorus assay. Anal Biochem 39:527–530.
- Mukherjee S, Maxfield FR. 2004. Membrane domains. Annu Rev Cell Dev Biol 20:839–866.
- Müller CP, Carey RJ, Huston JP, De Souza Silva MA. 2007. Serotonin and psychostimulant addiction: Focus on 5-HT1A-receptors. Prog Neurobiol 81:133–178.
- Ostrom RS, Insel PA. 2004. The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: Implications for molecular pharmacology. Br J Pharmacol 143:235–245.
- Paila YD, Chattopadhyay A. 2010. Membrane cholesterol in the function and organization of G-protein coupled receptors. Subcell Biochem 51:439–466.
- Paila YD, Ganguly S, Chattopadhyay A. 2010. Metabolic depletion of sphingolipids impairs ligand binding and signaling of human serotonin1A receptors. Biochemistry 49:2389–2397.
- Paila YD, Murty MRVS, Vairamani M, Chattopadhyay A. 2008. Signaling by the human serotonin1A receptor is impaired in cellular model of Smith-Lemli-Opitz syndrome. Biochim Biophys Acta 1778:1508–1516.
- Pediconi MF, Gallegos CE, De Los Santos EB, Barrantes FJ. 2004. Metabolic cholesterol depletion hinders cell-surface trafficking of the nicotinic acetylcholine receptor. Neuroscience 128:239–249.
- Pierce KL, Premont RT, Lefkowitz RJ. 2002. Seven-transmembrane receptors. Nat Rev Mol Cell Biol 3:639–650.
- Pike LJ, Casey L. 2002. Cholesterol levels modulate EGF receptor-mediated signaling by altering receptor function and trafficking. Biochemistry 41:10315–10322.
- Pucadyil TJ, Chattopadhyay A. 2004. Cholesterol modulates the ligand binding and G-protein coupling to serotonin1A receptors from bovine hippocampus. Biochim Biophys Acta 1663:188–200.
- Pucadyil TJ, Chattopadhyay A. 2006. Role of cholesterol in the function and organization of G-protein coupled receptors. Prog Lipid Res 45:295–333.
- Pucadyil TJ, Chattopadhyay A. 2007a. Cholesterol: A potential therapeutic target in Leishmania infection? Trends Parasitol 23:49–53.
- Pucadyil TJ, Chattopadhyay A. 2007b. Cholesterol depletion induces dynamic confinement of the G-protein coupled serotonin1A receptor in the plasma membrane of living cells. Biochim Biophys Acta 1768:655–668.
- Pucadyil TJ, Kalipatnapu S, Chattopadhyay A. 2005. The serotonin1A receptor: A representative member of the serotonin receptor family. Cell Mol Neurobiol 25:553–580.
- Pucadyil TJ, Kalipatnapu S, Harikumar KG, Rangaraj N, Karnik SS, Chattopadhyay A. 2004. G-protein-dependent cell surface dynamics of the human serotonin1A receptor tagged to yellow fluorescent protein. Biochemistry 43:15852–15862.
- Ramstedt B, Slotte JP. 2006. Sphingolipids and the formation of sterol-enriched ordered membrane domains. Biochim Biophys Acta 1758:1945–1956.
- Renner U, Glebov K, Lang T, Papusheva E, Balakrishnan S, Keller B, 2007. Localization of the mouse 5-hydroxytryptamine1Areceptor in lipid microdomains depends on its palmitoylation and is involved in receptor-mediated signaling. Mol Pharmacol 72:502–513.
- Rosenbaum DM, Rasmussen SGF, Kobilka BK. 2009. The structure and function of G protein-coupled receptors. Nature 459:356–363.
- Saxena R, Chattopadhyay A. 2011. Membrane organization and dynamics of the serotonin1A receptor in live cells. J Neurochem 116:726–733.
- Schlyer S, Horuk R. 2006. I want a new drug: G-protein-coupled receptors in drug development. Drug Discov Today 11:481–493.
- Schroeder RJ, Ahmed SN, Zhu Y, London E, Brown DA. 1998. Cholesterol and sphingolipid enhance the Triton X-100 insolubility of glycosylphosphatidylinositol-anchored proteins by promoting the formation of detergent-insoluble ordered membrane domains. J Biol Chem 273:1150–1157.
- Schuck S, Honsho M, Ekroos K, Shevchenko A, Simons K. 2003. Resistance of cell membranes to different detergents. Proc Natl Acad Sci USA 100:5795–5800.
- Shrivastava S, Pucadyil TJ, Paila YD, Ganguly S, Chattopadhyay A. 2010. Chronic cholesterol depletion using statin impairs the function and dynamics of human serotonin1A receptors. Biochemistry 49:5426–5435.
- Sjögren B, Csöregh L, Svenningsson P. 2008. Cholesterol reduction attenuates 5-HT1A receptor-mediated signaling in human primary neuronal cultures. Naunyn-chmiedeberg's Arch Pharmacol 378:441–446.
- Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, 1985. Measurement of protein using bicinchoninic acid. Anal Biochem 150:76–85.
- Subtil A, Gaidarov I, Kobylarz K, Lampson MA, Keen JH, McGraw TE. 1999. Acute cholesterol depletion inhibits clathrin-coated pit budding. Proc Natl Acad Sci USA 96:6775–6780.
- Troost J, Lindenmaier H, Haefeli WE, Weiss J. 2004. Modulation of cellular cholesterol alters P-glycoprotein activity in multidrug-resistant cells. Mol Pharmacol 66:1332–1339.
- Zhang Y, DeVries ME, Skolnick J. 2006. Structure modeling of all identified G protein coupled receptors in the human genome. PLoS Comput Biol 2:88–99.
- Zidovetzki R, Levitan I. 2007. Use of cyclodextrins to manipulate plasma membrane cholesterol content: Evidence, misconceptions and control strategies. Biochim BiophysActa 1768:1311–1324.