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Biocatalysis Volume 8, 1994 - Issue 4
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Original Article

Reaction Rates in Organic Media Show Similar Dependence on Water Activity with Lipase Catalyst Immobilized on Different Supports

Dauda K. Oladepo Department of Bioscience & Biotechnology, University of Strathclyde, Glasgow, G1 1XW, UK
,
Peter J. Hauling Department of Bioscience & Biotechnology, University of Strathclyde, Glasgow, G1 1XW, UK
&
Vidar F. Larsen Department of Chemical & Process Engineering, University of Strathclyde, Glasgow, G1 1XW, UK
Pages 283-287 | Received 12 Jun 1993, Published online: 11 Jul 2009

References

  • Adlercreutz P. On the importance of the support material for enzymatic synthesis in organic media. Support effects at controlled water activity. Eur. J. Biochem. 1991; 199: 609–614
  • Gupta M. N. Enzyme function in organic solvents. Eur. J. Biochem. 1992; 203: 25–32
  • Kawamoto T., Sonomoto K., Tanaka A. Esterification in solvents: Selection of hydrolases and effects of reaction conditions. Biocatalysis 1987; 1: 137–145
  • Mattiasson B., Adlercreutz P. Tailoring the microenvironment of enzymes in water-poor systems. Trends Biotechnol. 1991; 9: 394–398
  • Norin M., Boutelje J., Holmberg E., Hult K. Lipase immobilized by adsorption. Effect of support hydrophobicity on the reaction rate of ester synthesis in cyclohexane. Appl. Microb. Biotech. 1988; 28: 527–530
  • Reslow M., Adlercreutz P., Mattiasson B. On the importance of the support material for bioorganic synthesis. Influence of water partition between solvent, enzyme and solid support in water-poor reaction media. Eur. J. Biochem. 1988; 172: 573–578
  • Valivety R. H., Hailing P. J., Macrae A. R. Lipases from different sources vary widely in dependence of catalytic activity on water activity. Biochim. Biophys. Acta 1992a; 1122: 143–146
  • Valivety R. H., Hailing P. J., Macrae A. R. Reaction rate with suspended lipase catalyst shows similar dependence on water activity in different organic solvents. Biochim. Biophys. Acta 1992b; 1118: 218–222
  • Zaks A., Klibanov A. M. The effect of water on enzyme action in organic media. J. Biol. Chem. 1988; 263: 8017–8021

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