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Original Article

A rapid PCR dependent microtitre plate screening method for DNA sequences altered by site-directed mutagenesis

Pages 233-235 | Published online: 11 Jul 2009

References

  • Beck E., Ludwig G., Auerswald E. A., Reiss B., Schaller H. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 1982; 19: 327–336
  • Güssow D., Clackson T. Direct clone characterisation from plaques and colonies by the polymerase chain reaction. Nucl. Acids Res. 1989; 17: 4000
  • Kunkel T. A., Roberts J. D., Zakour R. A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 1987; 154: 367–382
  • Schofield J. P., Vaudin M., Kettle S., Jones D. S.C. A rapid semi-automated microtitre plate method for analysis and sequencing by PCR from bacterial stocks. Nucl. Acids Res. 1989; 17: 9498

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