63
Views
11
CrossRef citations to date
0
Altmetric
Original Article

A simple protocol for the automation of DNA cycle sequencing reactions and polymerase chain reactions

, &
Pages 17-23 | Received 11 Nov 1991, Published online: 11 Jul 2009

References

  • Carothers A. M., Urlaub G., Mucha J., Grunberger D., Chasin L. A. Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method. BioTechniques 1989; 7: 494–496, 498–499
  • Gyllensten U., Erlich H. A. Generation of single-strand DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc. Natl. Acad. Sci. U.S.A. 1988; 85: 7652–7656
  • Haff L., Atwood I. G., DiCesare J., Katz E., Picozza E., Williams J. F., Woudenberg T. A high performance system for the automation of the polymerase chain reaction. BioTechniques 1991; 10: 102–112
  • Kristensen T., Voss H., Ansorge W. A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing. Nucleic Acids Res. 1987; 15: 5507–5516
  • Mardis E. R., Roe B. A. Automated methods for single-stranded DNA isolation and dideoxynucleotide DNA sequencing reactions on a robotic workstation. BioTechniques 1989; 7: 840–850
  • Murray V. Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Res. 1989; 17: 8889
  • Wilson R. K., Yuen A. S., Clark S. M., Spence C., Arakelian P., Hood L. E. Automation of Dideoxynucleotide DNA Sequencing Reactions using a robotic workstation. BioTechniques 1988; 6: 776–787
  • Zimmermann I., Voss H., Schwader C., Stegemann I., Ansorge W. Automated Sanger dideoxy sequencing reaction protocol. FEBS 1988; 233: 432–436

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.