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DNA Sequence Volume 6, 1996 - Issue 4
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Original Article

Factors affecting the priming efficiency of short contiguous oligonucleotide strings in the primer walking strategy of DNA sequencing

T. Azhikina Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
,
M. Kostina Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
,
N. Skaptsova Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
,
V. Potapov Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
,
D. Berg Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
&
E. Sverdlov Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117871, Moscow, Russia; Department of Molecular Microbiology, Box 8230, Washington University School of Medicine, 4566 Scott Avenue, St Louis, MO, 63110, USA
Pages 211-216 | Received 04 Sep 1995, Published online: 11 Jul 2009

References

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  • Azhikina T., Veselovskaya S., Myasnikov V., Potapov V., Ermolayeva O., Sverdlov E. Strings of contiguous modified pentamers with increased DNA-binding affinity can be used for DNA sequencing by primer walking. Proc. Natl. Acad. Sci. USA 1993; 90: 11460–11462
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