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Original Article

A Protargol Method for Staining Nerve Fibers in Frozen or Celloidin Sections

Pages 27-33 | Published online: 12 Jul 2009

References

  • The initial work on the method was done in the laboratories of the Department of Anatomy of Cornell Medical College during the writer's tenure as a Rockefeller Fellow at Cornell. The author wishes to thank the members of the department at Cornell for their many courtesies, especially Dr. J. F. Nonidez whose extensive first-hand knowledge of the methods of Cajal and Bielschowsky enabled the writer to avoid many pitfalls in the early stages of the research
  • Bodian D. A new method for staining nerve fibers and nerve endings in mounted paraffin sections. Anat. Rec. 1936; 65: 89–97
  • Einarson L. A method for progressive selective staining of Nissl and nuclear substance in nerve cells. Amer. J. Path. 1932; 8: 295–307
  • In the staining solutions for steps 10 and 13 the author has used: a Griibler and a National Aniline sample of gallocyanin, anilin blue, and fast green FCF; a National Aniline as well as a Coleman and Bell sample of orange G. All the National Aniline stains were Commission certified (except gallocyanin, which has never been put on the certification basis). The chrome alum used was from Mallinchrodt
  • MeClung C. E. Microscopical Technique, 2nd Ed. Paul B. Hoeber Inc., New York 1937; 461–2, See pp.
  • Foley, James O. A section method for critical staining and differentiation of the unmyelinated fibers in peripheral nerves. Anat. Rec. 1939; 73: 65, Suppl.
  • Alexander W. F. The nerve plexus in the wall of the gall bladder. Anat. Rec., 79: 81, Suppl.

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