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Biotechnic & Histochemistry Volume 87, 2012 - Issue 1
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Original Article

The application of heated detergent dewaxing and rehydration to immunohistochemistry

AF HenwoodHistopathology Department, The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AustraliaCorrespondence[email protected]
Pages 46-50 | Accepted 13 Aug 2010, Published online: 21 Sep 2010

References

  • Buesa R, Peshkov MV (2009) Histology without xylene. Ann. Diag. Pathol. 13: 246–256.
  • Cuevas EC, Bateman AC, Wilkins BS, Johnson PA, Williams JH, Lee AHS, Jones DB, Wright DH (1994) Microwave antigen retrieval in immunocytochemistry: a study of 80 antibodies. J. Clin. Pathol. 47: 448–452.
  • Falkeholm L, Grant CA, Magnusson A, Möller E (2001) Xylene-free method for histological preparation: a multicentre evaluation. Lab. Invest. 81: 1213–1221.
  • Gomes L, Mackie N, Catchpoole D, Henwood T (2008) Test your knowledge – immunohistochemistry on 60 year old tissue blocks. J. Histotechnol. 31: 183–184.
  • Gray DS, Selbie D, Cooper RF, Williams M, Robson G, Doyle E (2006) Simultaneous de-waxing and standardisation of antigen retrieval in immunohistochemistry using commercially available equipment. Immunocytochemistry 4: 93–97.
  • Hazelbag HM, Broek JCM, Dorst EBL, Offerhaus GJA, Fleuren GJ, Hogendoorn PCW (1995) Immunostaining of chain-specific keratins on formalin-fixed, paraffin-embedded tissues: a comparison of various antigen retrieval systems using microwave heating and proteolytic pre-treatments. J. Histochem. Cytochem. 43: 429–437.
  • Henwood AF (1982) The importance of optimum titre in immunoperoxidase techniques. Aust. J. Med. Lab. Sci. 3: 21–27.
  • Henwood AF (1992) A comparison of S100, HMB-45 and NKI-C3 in melanocytic lesions: a preliminary study. Tissue Talk Jan.. 4–5.
  • Henwood AF (1995) A comparison of DAKO LCA and CLONAB ML in the differential diagnosis of lymphoma and other tumours. Aust. J. Med. Lab. Sci. 16: 164.
  • Henwood A (2005) Effect of slide drying at 80° C on immunohistochemistry. J. Histotechnol. 28: 45–46.
  • Leong AS-Y, Milios J (1993) An assessment of the efficacy of microwave-antigen retrieval procedure of the microwave antigen retrieval procedure on a range of tissue antigens. Appl. Immunohistochem. 1: 267–274.
  • O’Faolain E, Hunter MB, Byrne JM, Kelehan P, Lambkin HA, Byrne HJ, Lyng FM (2005) Raman spectroscopic evaluation of efficacy of current paraffin wax section dewaxing agents. J. Histochem. Cytochem. 53: 121–129.
  • Vlachos JD (1968) Birefringence and paraffinophilia of cell nuclei Stain Technol. 43: 89–95.

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