A simplified chromatin dispersion (nuclear halo) assay for detecting DNA breakage induced by ionizing radiation and chemical agents

References

• Amaral A (2005) Physical and biological dosimetry for risk perception in radioprotection. Braz. Arch. Biol. Technol. 48: 229–234.
   Google Scholar
• An MY, Kim TH (2002) Frequencies of micronuclei in peripheral lymphocytes in Korean populations after chronic low-dose radiation exposure. J. Vet. Sci. 3: 213–218.
   PubMedGoogle Scholar
• Andreetta AM, Stockert JC, Barrera C (1995) A simple method to detect sperm chromatin abnormalities: cytochemical mechanism and possible value in predicting semen quality in assisted reproductive procedures. Int. J. Androl. 18 (Suppl. 1): 23–28.
   Google Scholar
• Barrera C, Mazzolli AB, Pelling C, Stockert JC (1993) Metachromatic staining of human sperm nuclei after reduction of disulphide bonds. Acta Histochem. 94: 141–149.
   Google Scholar
• Cadet J, Douki T, Ravanat JL, Di Mascio P (2009) Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation. Photochem. Photobiol. Sci. 8: 903–911.
   PubMedGoogle Scholar
• Cameron IL, Prescott DM (1963) RNA and protein metabolism in the maturation of the nucleated chicken erythrocyte. Exp. Cell Res. 30: 609–612.
   PubMed Web of Science ®Google Scholar
• Chohan KR, Griffin JT, Lafromboise M, De Jonge CJ, Carrell DT (2006) Comparison of chromatin assays for DNA fragmentation evaluation in human sperm. J. Androl. 27: 53–59.
   PubMedGoogle Scholar
• Collins AR (2004) The comet assay for DNA damage and repair. Molec. Biotechnol. 26: 249–261.
   PubMed Web of Science ®Google Scholar
• Cook PR, Brazell IA (1976) Characterization of nuclear structures containing superhelical DNA. J. Cell Sci. 22: 303–324.
   PubMed Web of Science ®Google Scholar
• Cortés-Gutiérrez EI, Dávila Rodriguez MI, López-Fernández C, Fernández J, Gosálvez J (2007) Evaluación del daño en el ADN espermático. Actas Urol. Esp. 31: 120–131.
   Google Scholar
• Enciso M, López-Fernández C, Fernández JL, García P, Gosálbez A, Gosálvez J (2006) A new method to analyze boar sperm DNA fragmentation under bright-field or fluorescence microscopy. Theriogenology 65: 308–316.
   PubMed Web of Science ®Google Scholar
• Fairbairn D, Olive P, O’Neill K (1995) The comet assay: a comprehensive review. Mutat. Res. 339: 37–59.
   PubMed Web of Science ®Google Scholar
• Fernández JL, Muriel L, Goyanes V, Segrelles E, Gosálvez J, Enciso M, LaFromboise M, De Jonge C (2005) Simple detection of human sperm DNA fragmentation with an improved sperm chromatin dispersion test. Fertil. Steril. 84: 833–842.
   PubMed Web of Science ®Google Scholar
• Garaj-Vrhovac V, Kopjar N (2003) The alkaline comet assay as biomarker in assessment of DNA damage in medical personnel occupationally exposed to ionizing radiation. Mutagenesis 18: 265–271.
   PubMed Web of Science ®Google Scholar
• Gerdes MG, Carter KC, Moen PT, Lawrence JB (1994) Dynamic changes in the higher-level chromatin organization of specific sequences revealed by in situ hybridization to nuclear halos. J. Cell Biol. 126: 289–304.
   PubMed Web of Science ®Google Scholar
• Hall J, Giaccia Amato J (2006) Radiobiology for the Radiologist, 6th. Lippincott Williams & Wilkins, Philadelphia. 16–29.
   Google Scholar
• Hatz S, Lambert JDC, Ogilby PR (2007) Measuring the lifetime of singlet oxygen in a single cell: addressing the issue of cell viability. Photochem. Photobiol. Sci. 6: 1106–1116.
   PubMed Web of Science ®Google Scholar
• Kanda R (2000) Improvement of accuracy of chromosome aberration analysis for biological radiation dosimetry. J. Radiat. Res. 41: 1–8.
   PubMed Web of Science ®Google Scholar
• Léonard A, Rueff J, Gerber GB, Léonard ED (2005) Usefulness and limits of biological dosimetry based on cytogenetic methods. Radiat. Protect. Dosim. 115: 448–454.
   PubMed Web of Science ®Google Scholar
• Ljungman M (2009) Targeting the DNA damage response in cancer. Chem. Rev. 109: 2929–2950.
   PubMed Web of Science ®Google Scholar
• Lopes S, Sun JG, Jurisicova A, Meriano J, Casper RF (1998) Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil. Steril. 69: 528–532.
   PubMed Web of Science ®Google Scholar
• Matsunaga S, Ohshio K, Harada E, Fujiwara S, Uchiyama S, Fukui K (2004) Development of new dosimetry using extended DNA fibers. J. Biosci. Bioeng. 98: 384–386.
   Google Scholar
• McNair FI, Marples B, West CM, Moore JV (1997) A comet assay of DNA damage and repair in K562 cells after photodynamic therapy using haematoporphyrin derivative, methylene blue and meso-tetrahydroxyphenylchlorin. Br. J. Cancer 75: 1721–1729.
   Google Scholar
• Muriel L, Segrelles E, Goyanes V, Gosálvez J, Fernández JL (2004) Structure of human sperm DNA and background damage, analyzed by in situ enzymatic treatment and digital image analysis. Mol. Human Reprod. 10: 1–7.
   Google Scholar
• Neri M, Fucic A, Knudsen LE, Lando C, Merlo F, Bonassi S (2003) Micronuclei frequency in children exposed to environmental mutagens: a review. Mutat. Res. 544: 243–254.
   PubMed Web of Science ®Google Scholar
• Olive PL (1998) The role of DNA single- and double-strand breaks in cell killing by ionizing radiation. Radiat. Res. 150: S42–S51.
   PubMed Web of Science ®Google Scholar
• Pass B (1997) Collective radiation biodosimetry for dose reconstruction of acute accidental exposures: a review. Environ. Health Perspec. 105: 1397–1402.
   Google Scholar
• Paulson JR, Laemmli UK (1977) The structure of histone-depleted metaphase chromosomes. Cell 12: 817–828.
   PubMed Web of Science ®Google Scholar
• Pereira de Lemos Pinto MM, Frassinetti Gonçalvez Santos N, Amaral A (2010) Current status of biodosimetry based on standard cytogenetic methods. Rad. Environ. Biophys. 49: 567–581.
   PubMed Web of Science ®Google Scholar
• Piette J (1991) Biological consequences associated with DNA oxidation mediated by singlet oxygen. J. Photochem. Photobiol. B: Biol. 11: 241–260.
   PubMed Web of Science ®Google Scholar
• Sestili P, Cantoni O, Cattabeni F, Murray D (1995) Evidence for separate mechanisms of cytotoxicity in mammalian cells treated with hydrogen peroxide in the absence or presence of L-histidine. Biochim. Biophys. Acta 1268: 130–136.
   PubMed Web of Science ®Google Scholar
• Sestili P, Cantoni O (1999) Osmotically driven radial diffusion of single-stranded DNA fragments on an agarose bed as a convenient measure of DNA strand scission. Free Rad. Biol. Med. 26: 1019–1026.
   PubMed Web of Science ®Google Scholar
• Sestili P, Martinelli C, Stocchi V (2006) The fast halo assay: an improved method to quantify genomic DNA strand breakage at the single-cell level. Mutat. Res. 607: 205–214.
   PubMed Web of Science ®Google Scholar
• Sidebottom E (1974) Heterokaryons and their use in studies of nuclear function. The Cell Nucleus, vol. 1, Busch H, Acad. Press, New York, London. 439–469.
   Google Scholar
• Sies H, Menck CFM (1992) Singlet oxygen induced DNA damage. Mutat. Res. 275: 367–375.
   PubMed Web of Science ®Google Scholar
• Singh NP (2000) A simple method for accurate estimation of apoptotic cells. Exp. Cell. Res. 256: 328–337.
   PubMed Web of Science ®Google Scholar
• Stoilov LM, Mullenders LHF, Darroudi F, Natarajan AT (2007) Adaptive response to DNA and chromosomal damage induced by X-rays in human blood lymphocytes. Mutagenesis 22: 117–122.
   PubMed Web of Science ®Google Scholar
• Streffer C, Müller WU, Kryscio A, Böcker W (1998) Micronuclei-biological indicator for retrospective dosimetry after exposure to ionizing radiation. Mutat. Res. 404: 101–105.
   PubMedGoogle Scholar
• Stricklin D, Jaworska A, Arvidsson E (2007) Method for efficient establishment of technical biodosimetry competence. Radiat. Meas. 42: 1114–1118.
   Web of Science ®Google Scholar
• Vogelstein B, Pardoll DM, Coffey DF (1980) Supercoiled loops and eukaryotic DNA replication. Cell 22: 79–85.
   PubMed Web of Science ®Google Scholar
• Wiegant J, Kalle W, Mullenders LS, Hovers JMN, Dauwerse JG, van Ommen GJB, Raap AK (1992) High-resolution in situ hybridization using DNA halo preparations. Hum. Molec. Genet. 1: 587–591.
   PubMed Web of Science ®Google Scholar
• Wood DK, Weingeist DM, Bathia SN (2010) Single cell trapping and DNA damage analysis using microwell arrays. Proc. Nat. Acad. Sci. USA 107: 10008–10013.
   PubMed Web of Science ®Google Scholar