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Original Article

Propidium iodide for making heterochromatin more evident in the C-banding technique

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Pages 433-438 | Accepted 23 Apr 2012, Published online: 02 Jul 2012

References

  • Arndt-Jovin DJ, Jovin TM (1989) Fluorescence labeling and microscopy of DNA. Methods Cell Biol. 30: 417–448.
  • Arrighi FE, Hsu TC (1971) Localization of heterochromatin in human chromosomes. Cytogenetics 10: 81–86.
  • Barros e Silva AE, Guerra M (2010) The meaning of DAPI bands observed after C-banding and FISH procedures. Biotech. & Histochem. 85: 115–125.
  • Bellafronte E, Vicari MR, Artoni RF, Margarido VP, Moreira-Filho O (2009) Differentiated ZZ/ZW sex chromosomes in Apareiodon ibitiensis (Teleostei, Parodontidae): cytotaxonomy and biogeography. J. Fish Biol. 75: 2313–2325.
  • Bertollo LAC, Takahashi CS, Moreira-Filho O (1978) Cytotaxonomic considerations on Hoplias lacerdae (Pisces, Erythrinidae). Braz. J. Genet. 2: 103–120.
  • Blanco DR, Lui RL, Bertollo LAC, Margarido VP, Moreira-Filho O (2010) Karyotypic diversity between allopatric populations of the group Hoplias malabaricus (Characiformes: Erythrinidae): evolutionary and biogeographic considerations. Neotrop. Ichthyol. 8: 361–368.
  • Bresloff JL, Crothers DM (1981) Equilibrium studies of ethidium-polynucleotide interactions. Biochemistry 20: 3547–3553.
  • Camacho JPM (2005) B Chromosomes. In: Gregory TR, The Evolution of the Genome, Elsevier, San Diego, CA. pp. 223–286.
  • Castiglia R, Garagna S, Merico V, Oguge N, Corti M (2006) Cytogenetics of a new cytotype of African Mus (subgenus Nannomys) minutoides (Rodentia, Muridae) from Kenya: C- and G- banding and distribution of (TTAGGG)n telomeric sequences. Chromosome Res. 14: 587–594.
  • Catarino S, Alvarez E, Campa A, Vieira R, Roca A, Giraldez R (2006) Identification and physical mapping of induced translocation breakpoints involving chromosomes 1R in rye. Chromosome Res. 14: 755–765.
  • Centofante L, Bertollo LAC, Moreira-Filho O (2002) A ZZ/ZW sex chromosomes system in a new species of the genus Parodon (Pisces, Parodontidae). Caryologia 55: 139–150.
  • Chandley AC, Fletcher JM (1973) Centromere staining at meiosis in man. Humangenetik 18: 247–252.
  • Comings DE (1978) Mechanisms of chromosome banding and implications for chromosome structure. Ann. Rev. Genet. 12: 25–46.
  • Dillon N (2004) Heterochromatin structure and function. Biol. Cell 96: 631–637.
  • Fedoroff N (2000) Transposons and genome evolution in plants. Proc. Natl. Acad. Sci. USA 97: 7002–7007.
  • Fregonezi JN, Fernandes T, Torezan JMD, Vieira AOS, Vanzela ALL (2006) Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA. Genet. Mol. Biol. 29: 97–104.
  • Garcia C, Oliveira C, Almeida-Toledo LF (2010) Karyotypic evolution trends in Rhamdia quelen (Siluriformes, Heptapteridae) with considerations about the origin and differentiation of its supernumerary chromosomes. Genet. Mol. Res. 9: : 365–384.
  • Gold JR, Amemiya CT, Ellison JR (1986) Chromosomal heterochromatin differentiation in North American Cyprinid fishes. Cytologia 51: 557–566.
  • Gold JR, Li YC, Shipley NS, Powers PK (1990) Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J. Fish Biol. 37: : 563–575.
  • Hennig W (2004) The revolution of the biology of the genome. Cell Res. 14: 1–7.
  • Holmquist G (1979) The mechanism of C-banding: depurination and β-elimination. Chromosoma 72: 203–224.
  • Jack EM, Harrison CJ, Allen TD, Harris R (1985) The structural basis for C-banding. A scanning electron microscopy study. Chromosoma 91: 363–368.
  • Kashkush K, Feldman M, Levy AA (2003) Transcriptional activation of retrotransposons alters the expression of adjacent genes in wheat. Nat. Genet. 33: 102–106.
  • Kawagoshi T, Nishida C, Ota H, Kumazawa Y, Endo H, Matsuda Y (2008) Molecular structures of centromeric heterochromatin and karyotypic evolution in the Siamese crocodile (Crocodylus siamensis) (Crocodylidae, Crocoylia). Chromosome Res. 16: 1119–1132.
  • Kim ES, Punina EO, Rodionov AV (2002) Chromosome CPD (PI/DAPI)- and CMA/DAPI-banding patterns in Allium cepa L. Russ. J. Genet. 38: 489–496.
  • Koehler MR, Dehm D, Guttenbach M, Nanda I, Haaf T, Molina WF, Galetti PM Jr, Schmid M (1997) Cytogenetics of the genus Leporinus (Pisces, Anostomidae). 1. Karyotype analysis, heterochromatin distribution and sex chromosomes. Chromosome Res. 5: 12–22.
  • Kongsuwan K, Smyth DR (1978) DNA loss during C-banding of chromosomes of Lilium longiflorum. Chromosoma 68: 59–72.
  • Kuipers AGJ, van Os DPM, Hans de Jong J, Ramanna MS (1997) Molecular cytogenetics of Alstroemeria: identification of parental in interspecific hybrids and characterization of repetitive DNA families in constitutive heterochromatin. Chromosome Res. 5: 31–39.
  • Lui RL, Blanco DR, Margarido VP, Moreira-Filho O (2010) Chromosome characterization and biogeographic relations among three populations of the driftwood catfish Parauchenipterus galeatus (Linnaeus, 1766) (Siluriformes: Auchenipteridae) in Brazil. Biol. J. Linnean Soc. 99: 648–656.
  • Macaisne N, Dutrillaux AM, Dutrillaux B (2006) Meiotic behaviour of a new complex X-Y-autosome translocation and amplified heterochromatin in Jumnos ruckeri (Saunders) (Coleoptera, Scarabaeidae, Cetoniinae). Chromosome Res. 14: 909–918.
  • Marky LA (1990) Hydration of dA·dT polymers: role of water in the thermodynamics of ethidium and propidium intercalation. Biochemistry 29: 4805–4811.
  • Martinez JF, Lui RL, Blanco DR, Traldi JB, Silva LF, Venere PC, Souza IL, Moreira-Filho O (2011) Comparative cytogenetics of three populations from the Rhamdia quelen complex (Siluriformes, Heptapteridae) in two Brazilian hydrographic basins. Caryologia 64: 121–128.
  • Mazzini G, Giordano P, Riccardi A, Montecucco M (1983) A flow cytometric study of the propidium iodide staining kinetics of human leukocytes and its relationships with chromatin structure. Cytometry 3: 443–448.
  • Meisner LF, Chuprevich TW, Inhorn SL, Johnson CB (1974) Dye-nucleoprotein interactions in Giemsa banding. J. Cell Biol. 61: 248–252.
  • Molina WF, Schmid M, Galetti PM Jr (1998) Heterochromatin and sex chromosomes in the neotropical fish genus Leporinus (Characiformes, Anostomidae). Cytobios 94: 141–149.
  • Moreira-Filho O, Bertollo LAC, Galetti Jr PM (1993) Distribution of sex chromosome mechanisms in neotropical fish and description of a ZZ/ZW system in Parodon hilarii (Parodontidae). Caryologia 46: 115–125.
  • Muller W, Crothers DM (1975) Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands. Eur. J. Biochem. 54: 267–277.
  • Nafisi S, Saboury AA, Keramat N, Neault JF, Tamijir-Riahi HA (2007) Stability and structure features of DNA intercalation with ethidium bromide, acridine orange and methylene blue. J. Mol. Struct. 827: 35–43.
  • Néo DM, Bertollo LAC, Moreira-Filho O (2000) Morphological differentiation and possible origin of B chromosomes in natural Brazilian population of Astyanax scabripinnis (Pisces, Characidae). Genetica 108: 211–215.
  • Oliveira C, Wright JM (1998) Molecular cytogenetic analysis of heterochromatin in the chromosomes of tilapia, Oreochromis niloticus (Teleostei: Cichlidae). Chromosome Res. 6: 205–211.
  • Oliveira C, Foresti F, Hilsdorf AWS (2009) Genetics of neotropical fish: from chromosomes to populations. Fish Physiol. Biochem. 35: 81–100.
  • Olmsted J III, Kearns DR (1977) Mechanism of ethidium bromide fluorescence enhancement on binding to nucleic acids. Biochemistry 16: 3647–3654.
  • Pardue ML, Gall JG (1970) Chromosomal localization of mouse satellite DNA. Science 168: 1356–1358.
  • Pardue ML, Hennig W (1990) Heterochromatin: junk or collectors’ item? Chromosoma 100: 3–7.
  • Peterson DG, Lapitan NLV, Stack SM (1999) Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152: 427–439.
  • Ren J, Jenkins TC, Chaires JB (2000) Energetics of DNA intercalation reactions. Biochemistry 39: 8439–8447.
  • Rocchi A (1982) On the heterogeneity of heterochromatin. Caryologia 35: 169–189.
  • Rosa R, Bellafronte E, Moreira-Filho O, Margarido VP (2006) Constitutive heterochromatin, 5S and 18S rDNA genes in Apareiodon sp. (Characiformes, Parodontidae) with a ZZ/ZW sex chromosomes system. Genetica 128: 159–166.
  • She CW, Liu JY, Song YC (2006) CPD staining: an effective technique for detection of NORs and other GC-rich chromosomal regions in plants. Biotechnic. & Histochem. 81: 13–21.
  • Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp. Cell Res. 75: 304–306.
  • Sumner AT (1990) Chromosome Banding. Unwin-Hyman, London. pp. 39–69.
  • Sumner AT (2003) Chromosomes: Organization and Function. Blackwell Publishing, London. pp. 24–132.
  • Sumner AT, Evans HJ, Buckland RA (1971) New technique for distinguishing between human chromosomes. Nature (New Biol.). 232: 3l–32.
  • Tanious FA, Veal JM, Buczak H, Ratmeyer LS, Wilson WD (1992) DAPI (4’, 6’-Diamidino-2-phenylindole) binds differently to DNA and RNA: minor-groove binding at AT sites and intercalation at AU sites. Biochemistry 31: 3103–3112.
  • Treco FR, Malabarba LR, Giuliano-Caetano L, Dias AL (2008) Cytogenetic study of two species of the family Pimelodidae (Siluriformes) collected in Lago Guaíba, Rio Grande do Sul, Brazil. Neotrop. Ichthyol. 6: 87–92.
  • van Dujin P, van Prooijen-Knegt AC, van der Ploeg M (1985) The involvement of nucleosomes in Giemsa staining of chromosomes. A new hypothesis on the banding mechanism. Histochemistry 82: 363–376.
  • Vosa CG (1976) Heterochromatin classification in Vicia faba and Scilla sibirica. Chrom. Today. 5: 185–192.
  • Wahl Ph, Paoletti J, Le Pecq JB (1970) Decay of fluorescence emission anisotropy of the ethidium bromide-DNA complex evidence for an internal motion in DNA. Proc. Nat. Acad. Sci. USA 65: 417–421.
  • Waring MJ (1965) Complex formation between ethidium bromide and nucleic acids. J. Mol. Biol. 13: 269–282.
  • Waring MJ (1968) Drugs which affect the structure and function of DNA. Nature 219: 1320–1325.
  • Yunis JJ, Yasmineh WG (1971) Heterochromatin, satellite DNA, and cell function. Structural DNA of eukaryotes may support and protect genes and aid in speciation. Science 174: 1200–1209.
  • Zuckerkandl E, Hennig W (1995) Tracking heterochromatin. Chromosoma 104: 75–83.

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