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Stain Technology Volume 41, 1966 - Issue 3
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Original Article

Rapid Schedules for Koh Clearing and Alizarin Red S Staining of Fetal Rat Bone

Ronald P. Jensh Departments of Anatomy, Radiology, Pediatrics and The Eleanor Roosevelt Research Laboratories, Stein Research Center, Jefferson Medical College, Philadelphia, Pennsylvania, 19107
&
Robert L. Brent Departments of Anatomy, Radiology, Pediatrics and The Eleanor Roosevelt Research Laboratories, Stein Research Center, Jefferson Medical College, Philadelphia, Pennsylvania, 19107
Pages 179-183 | Published online: 12 Jul 2009

References

  • Burdi A. R. Toluidine blue-alizarin red S staining of cartilage and bone in whole-mount skeletons in vitro. Stain Techn. 1965; 40: 45–8
  • Conn H. J., Darrow, Mary A., Emmel V. M. Staining Procedures. Williams & Wilkins Co., Baltimore 1960
  • Crary D. D. Modified benzyl alcohol clearing of alizarin-stained specimens without loss of flexibility. Stain Techn. 1962; 37: 124–5
  • Cumley R. W., Crow J. F., Griffin A. B. Clearing specimens for demonstration of bone. Stain Techn. 1939; 14: 7–11
  • Dawson A. B. A note on the staining of the skeleton of cleared specimens with alizarin red S. Stain Techn. 1926; 1: 123–4
  • Green M. C. A rapid method for clearing and staining specimens for the demonstration of bone. Ohio J. Sci. 1952; 52: 31–3
  • Hood R. C. W. S., Neill W. A modification of alizarin red S technic for demonstrating bone formation. Stain Techn. 1948; 23: 209–18
  • Hoyte D. A. N. Alizarin as an indicator of bone growth. J. Anat., London 1960; 94: 432–42
  • Lipman H. J. Staining the skeleton of cleared embryos with alizarin red S. Stain Techn. 1935; 10: 61–3
  • Mall F. P. On ossification centers in human embryos less than 100 days old. Amer. J. Anat. 1906; 5: 433–58
  • Noback C. R., Noback Eleanor. Demonstrating the osseous skeleton of human embryos and fetuses. Stain Techn. 1944; 19: 51–3
  • Richmond G. W., Bennett L. Clearing and staining of embryos for demonstrating ossification. Stain Techn. 1938; 13: 77–9
  • Schultze O. Über Herstellung und Conservirung durchsichtiger Embryonen zum Studium. Anat. Gesselsch. 1897; 11: 3–5
  • Sedra S. N. Decreasing the time required for making an alizarin skeleton preparation. Stain Techn. 1950; 25: 223–4
  • Amand G. S., St., Amand W., St. Shortening maceration time for alizarin red S preparations. Stain Techn. 1950; 26: 271
  • Staples R. E., Schnell, Viola L. Refinements in rapid clearing technic in the KOH-alizarin red S method for fetal bone. Stain Techn. 1964; 39: 61–3
  • Williams T. W. Alizarin red S and toluidine blue for differentiating adult and embryonic bone and cartilage. Stain Techn. 1941; 16: 23–5

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