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Original Article

The Toluidine Blue-Membrane Filter Method: Absorption Spectra of Toluidine Blue Stained Bacterial Cells and the Relationship Between Absorbance and Dry Mass of Bacteria

Pages 357-364 | Published online: 12 Jul 2009

References

  • Bartholomew J. W., Finkelstein H. Crystal violet binding capacity and the Gram reaction of bacterial cells. J. Bacteriol 1954; 67: 689–691
  • Borzani W., Vairo M. L. R. Quantitative adsorption of methylene blue by dead yeast cells. J. Bacteriol 1958; 76: 251–255
  • Borzani W., Vairo M. L. R. Adsorption of methylene blue as a means of determining cell concentration of dead bacteria in suspensions. Stain Technol 1960; 35: 77–81
  • de Man J. C., Rogosa M., Sharpe M. E. A medium for the cultivation of lactobacilli. J. Appl. Bacteriol 1960; 23: 130–135
  • Ewing G. W. Instrumental Methods of Chemical Analysis, 3rd ed. McGraw-Hill, New York 1969
  • Finkelstein H., Bartholomew J. W. Dye uptake by Gram-positive and Gram-negative cells as related to adsorption laws. J. Bacteriol 1960; 80: 14–17
  • Fischer J., Romhányi G. Optical studies on the molecular-sterical mechanism of metachromasia. Acta Histochem 1977; 59: 29–39
  • Kennedy E. R., Woodhur A. F. Quantitative studies of differential staining reactions. I. The effect of pH on the quantity of dye retained by bacteria and the apparent isoelectric point. J. Bacteriol 1956; 72: 447–450
  • Koch A. L. Turbidity measurements of bacterial cultures in some available commercial instruments. Anal. Biochem 1970; 38: 252–259
  • Lawrence J. V., Maier S. Correction for the inherent error in optical density readings. Appl. Environ. Microbiol 1977; 33: 482–484
  • McKamey M. R., Spitznagle L. A. Chromatographic, mass spectral, and visible light absorption characteristics of toluidine blue O and related dyes. J. Pharm. Sci 1975; 64: 1456–1462
  • Mallette M. F. Evaluation of growth by physical and chemical means. Methods in Microbiology, vol. 1, J. R. Norris, D. W. Ribbons. Academic Press, London and New York 1969; 521–566
  • Moats W. A. Staining of bacteria in milk for direct microscopic examination—a review. J. Milk Food Technol 1964; 27: 308–310
  • O'Toole D. K. A toluidine blue-membrane filter method for the quantitative staining of bacteria. Stain Technol 1983; 58: 292–298
  • O'Toole D. K. A weighing technique for determining the dry mass of bacteria based on rate of moisture uptake. Appl. Environ. Microbiol 1983; 46: 506–508
  • Pirt S.J. Principles of Microbe and Cell Cultivation. Blackwell Scientific Publications, Melbourne 1975; 4–21
  • Sartorius A. Cellulose Acetate Strips for Electrophoresis, SMIO. Sartorious-Membranfilter GmbH, Göttingen 1971
  • Sokal R. R., Rohlf F. J. Biometry: the Principles and Practice of Statistics in Biological Research. W.H. Freeman, San Francisco 1969; 405–493
  • Steel R. G. D., Torrie J. H. Principles and Procedures of Statistics with Special Reference to the Biological Sciences. McGraw-Hill, London 1960
  • Terzaghi B. E., Sandine W. E. Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol 1975; 29: 807–813
  • Wilson G. S., Twigg R. S., Wright R. C., Hendry C. B., Cowell M. P., Maier I. The Bacteriological Grading of Milk: a Critical Study, Medical Research Council, Special Report Series No. 206. His Majesty's Stationery Office, London 1935; 392
  • Wolman M. Amyloid, its nature and molecular structure: comparison of new toluidine blue polarized light method with traditional procedures. Lab. Invest 1971; 25: 104–110

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