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Biotechnic & Histochemistry Volume 70, 1995 - Issue 1
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Original Article

Improved Lipid Visualization with a Modified Osmium Tetroxide Method Using Ultrasonic Treatment and Intensification with Imidazole or Triazole

Rieko Nishimura Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 570, Japan
,
Iezo Nakao Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 570, Japan
,
Nobuaki Shikata Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 570, Japan
,
Airo Tsubura Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 570, Japan
&
Sotokichi Morii Department of Pathology, Kansai Medical University, Moriguchi, Osaka, 570, Japan
Pages 28-32 | Published online: 12 Jul 2009

References

  • Angermüller S., Fahimi H. D. Imidazole-buffered osmium tetroxide: an excellent stain for visualization of lipids in transmission electron microscopy. Histochem. J. 1982; 14: 823–835
  • Cain A. J. The histochemistry of lipids in animals. Biol. Rev. 1950; 25: 73–112
  • Hayat M. A. Stains and Cytochemical Methods. Plenum Press, New York 1993; 168–172.
  • Lison L. Histochimie et Cytochimie Animales, Principes et Méthodes. Gauthier-Villars, Paris 1953
  • Morii S., Shikata N., Nakao I. Ultracytochemistry of cytoplasmic lipid droplets. Acta Histochem. Cytochem. 1993; 26: 251–259
  • Nakao I., Okada H., Senzaki H., Nishimura R, Fujita N., Shikata N., Morii S. Amixture of paraphenylenediamine and imidazole: its effect on the extraction of lipid droplets during electron microscopy staining. Biotechnic & Histochemistry 1992; 67: 219–223
  • Palade G. E. A study of fixation for electron microscopy. J. Exp. Med. 1952; 95: 285–298
  • Yasuda K., Yamashita S., Shiozawa M., Aiso S., Yasui Y. Application of ultrasound for tissue fixation: combined use with microwave to enhance the effect of chemical fixation. Acta Histochem. Cytochem. 1992; 25: 237–244

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