50
Views
11
CrossRef citations to date
0
Altmetric
Original Article

Anti-Fading Agents for Confocal Immunofluorescence: Colocalization of Nuclear Polypeptides

&
Pages 40-45 | Published online: 12 Jul 2009

References

  • Allis C. D., Waring G. L., Mahowald A. P. Mass isolation of pole cells from Drosophila melanogaster Dev. Biol 1977; 56: 372–381.
  • Berrios M., Osheroff N., Fisher P. A. In situ localization of DNA topoisomerase II, a major polypeptide component of the Drosophila nuclear matrix fraction. Proc. Natl. Acad. Sci. USA 1985; 82: 4142–4146.
  • Berrios M., Fisher P. A. A myosin heavy chain-like polypeptide is associated with the nuclear envelope in higher eukaryotlc cells. J. Cell Biol. 1986; 103: 711–724
  • Berrios M. A new design of immunofluorescence chamber. Med. Lab. Sci. 1989; 46: 276–278
  • Berrios M., Fisher P. A., Matz E. C. Localization of a myosin heavy chain-like polypeptide to Drosophila nuclear pore complexes. Proc. Natl. Acad. Sci. USA 1991; 88: 219–223.
  • Berrios M. Device to prepare extruded nuclei and chromosome squashes. Biotechnic & Histochemistry 1994; 69: 78–80
  • Bock G., Hilchenbach M., Schauenstein K., Wick G. Photometric analysis of antifading reagents for immunofluorescence with laser and conventional illumination sources. J. Histochem. Cytochem. 1985; 33: 699–705
  • Fisher P. A. Chromosomes and chromatin structure: the extrachromosomal karyoskeleton. Curr. Opinion Cell Biol. 1989; 1: 447–453
  • Giloh H., Sedat J. W. Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science 1982; 217: 1252
  • Johnson G. D., Araujo G. M. C. A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 1981; 43: 349–350
  • Kaufman G. I., Nester J. F., Wasserman D. E. An experimental study of lasers as excitation source for automated fluorescence antibody instrumentation. J. Histochem. Cytochem. 1971; 19: 469–476
  • Mirkovitch J., Gasser S. M., Laemmli U. K. Relation of chromosome structure and gene expression. Philos. Trans. R. Soc. Lond. (B). 1987; 317: 563–574
  • Pardue M. L. In situ hybridization to DNA of chromosomes and nuclei. Drosophila: A Practical Approach., D. B. Roberts. IRL Press, Oxford 1986; 111–137.
  • Rigaut J. P., Vassy J. High-resolution three-dimensional images from confocal scanning laser microscopy. Quantitative study and mathematical correction of the effects from bleaching and fluorescence attenuation in depth. Anal. Quant. Cytol. Hlstol. 1991; 13: 223–232
  • Schmidt T., Chen P. S., Pellagrini M. The induction of ribosome biosynthesis in a nonmitotic secretory tissue. J. Biol. Chem. 1985; 260: 7645–7650
  • Smith D. E., Gruenbaum Y., Berrios M., Fisher P. A. Biosynthesis and interconversion of Drosophila nuclear lamin isoforms during normal growth and in response to heat shock. J. Cell Biol 1987; 105: 771–790.
  • Webb J. P., McColgin W. C., Peterson O. G., Stockman D. L., Eberly J. H. Intersystem crossing rate and triplet state lifetime for a lasing dye. J. Chem. Phys. 1970; 53: 4227–4229
  • Whalen A. M., McConnell M., Fisher P. A. Developmental regulation of Drosophila DNA topoisomerase II. J. Cell Biol 1991; 112: 203–213.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.