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Original Article

An Automated Technique for Double Staining Rat and Rabbit Fetal Skeletal Specimens to Differentiate Bone and Cartilage

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Pages 98-104 | Received 28 Jan 1998, Accepted 25 Nov 1998, Published online: 12 Jul 2009

References

  • Dawson A. B. A note on the staining of the skeleton of cleared specimens with alizarin red S. Stain Technol. 1926; 1: 123–124
  • United States Environmental Protection Agency. Health Effects Test Guidelines OPPTS 870.3700 Prenatal Developmental Toxicity Study 1998
  • Inouye M. Differential staining of cartilage and bone in fetal mouse skeleton by alcian blue and alizarin red S. Congenital Anom. 1976; 16: 171–173
  • Kimmel C. A., Trammell C. A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. Stain Tech-nol. 1981; 56: 271–273
  • Miller D. M., Tarpley J. An automated double staining procedure for bone and cartilage. Biotech. & Histochem. 1996; 71: 79–83
  • Rousseaux C. G. Automated differential staining for cartilage and bone in whole mount preparations of vertebrates. Stain Technol. 1985; 60: 295–296
  • Staples R. E., Schnell V. L. Refinements in rapid clearing technique in the KOH-alizarin red S method for fetal bone. Stain Technol. 1964; 39: 61–63
  • Whitaker J., Dix K. M. Double staining technique for rat fetus skeletons in teratological studies. Lab. Anim. 1979; 13: 309–310

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