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Research Article

Hoechst 33342 induces radiosensitization in malignant glioma cells via increase in mitochondrial reactive oxygen species

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Pages 936-949 | Received 11 Mar 2010, Published online: 14 Jun 2010

References

  • Chen AY, Yu C, Bodley A, Peng LF, Liu LF. A new mammalian DNA topoisomerase I poison Hoechst 33342: cytotoxicity and drug resistance in human cell cultures. Cancer Res 1993;53:1332–1337.
  • Chen AY, Yu C, Gatto B, Liu LF. DNA minor groove-binding ligands: a different class of mammalian DNA topoisomerase I inhibitors. Proc Natl Acad Sci USA 1993;90:8131–8135.
  • Kiechle FL, Zhang X. Hoechst 33342 induces apoptosis and alters TATA box binding protein/DNA complexes in nuclei from HL-60 cells. J Clin Ligand Assay 1998;21:413–417.
  • Zhang X, Kiechle FL. Hoechst 33342–induced apoptosis is associated with intracellular accumulation of E2F-1 Protein in BC3H-1 myocytes and HL-60 cells. Arch Path Lab Med 2001;125:99–104.
  • Adhikari JS, Khaitan D, Arya MB, Dwarakanath BS. Heterogeneity in the radiosensitizing effects of the DNA ligand hoechst-33342 in human tumor cell lines. J Cancer Res Ther 2005;1:151–161.
  • Tawar U, Jain AK, Chandra R, Singh Y, Dwarakanath BS, Chaudhury NK, Good L, Tandon V. Minor groove binding DNA ligands with expanded A/T sequence length recognition, selective binding to bent DNA regions and enhanced fluorescent properties. Biochemistry 2003;42:13339–13346.
  • Ren J, Chaires JB. Sequence and structural selectivity of nucleic acid binding ligands. Biochemistry 1999;38: 16067–16075.
  • Haq I, Ladbury JE, Chowdhry BZ, Jenkins TC, Chaires JB. Specific binding of Hoechst 33258 to the d (CGCAAATTT GCG) 2 duplex: calorimetric and spectroscopic studies. J Mol Biol 1997;271:244–257.
  • Singh KK, Russell J, Sigala B, Zhang Y, Williams J, Keshav KF. Mitochondrial DNA determines the cellular response to cancer therapeutic agents. Oncogene 1999;18:6641–6646.
  • Chen JC, Zhang X, Singleton TP, Kiechle FL. Mitochondrial membrane potential change induced by Hoechst 33342 in myelogenous leukemia cell line HL-60. Ann Clin Lab Sci 2004; 34:458–466.
  • Zhang X, Kiechle FL. Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes. Ann Clin Lab Sci 2006;36:185–193.
  • Mattson DM, Ahmad IM, Dayal D, Parsons AD, Aykin-Burns N, Li L, Orcutt KP, Spitz DR, Dornfeld KJ, Simons AL. Cisplatin combined with zidovudine enhances cytotoxicity and oxidative stress in human head and neck cancer cells via a thiol-dependent mechanism. Free Radic Biol Med 2009; 46:232–237.
  • Dwarkanath BS, Jain VK. Energy linked modifications of the radiation response in a human cerebral glioma cell line. Int J Radiat Oncol Biol Phys 1989;17:1033–1040.
  • Tamura K, Aotsuka T. Rapid isolation method of animal mitochondrial DNA by the alkaline lysis procedure. Biochem Genet 1988;26:815–819.
  • Chaudhury NK, Bhardwaj R. Structural stabilization by Hoechst 333258 in γ-irradiated DNA: evidenced by spectroscopic studies. Curr Sci 2004;87:1256–1262.
  • Rothe G, Valet G. Flow cytometric analysis of respiratory burst activity in phagocytes with hydroethidine and 2′, 7′-dichlorofluorescin. J Leukocyte Biol 1990;47:440–448.
  • Narayanan PK, Goodwin EH, Lehnert BE. Alpha particles initiate biological production of superoxide anions and hydrogen peroxide in human cells. Cancer Res 1997;57:3963–3971.
  • Zölzer F, Hillebrandt S, Streffer C. Radiation induced G1-block and p53 status in six human cell lines. Radioth Oncol 1995;37:20–28.
  • Vermes I, Haanen C, Steffens-Nakken H, Reutellingsperger C. A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V. J Immunol Methods 1995; 184:39–51.
  • Lee HC, Yin PH, Lu CY, Chi CW, Wei YH. Increase of mitochondria and mitochondrial DNA in response to oxidative stress in human cells. Biochem J 2000;348:425–432.
  • Maftah A, Ratinaud M, Dumas M, Bonte F, Meybeck A, Julien R. Human epidermal cells progressively lose their cardiolipins during ageing without change in mitochondrial transmembrane potential. Mech Ageing Dev 1994;77:83–96.
  • Harshman KD, Dervan PB. Molecular recognition of B-DNA by Hoechst 33258. Nucleic Acids Res 1985;13:4825–4835.
  • Andrews RM, Kubacka I, Chinnery PF, Lightowlers RN, Turnbull DM, Howell N. Reanalysis and revision of the cambridge reference sequence for human mitochondrial DNA. Nat Genet 1999;23:147.
  • Varshney R, Dwarakanath BS, Jain V. Radiosensitization by 6-aminonicotinamide and 2-deoxy-D-glucose in human cancer cells. Int J Radiat Biol 2005;81:397–408.
  • Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC. Photophysical properties of fluorescent DNA-dyes bound to single-and double-stranded DNA in aqueous buffered solution. Photochem Photobiol 2001;73:585–599.
  • Jin R, Breslauer KJ. Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly [d (AT)]. poly [d (AT)] duplex. Proc Natl Acad Sci USA 1988;85:8939–8942.
  • Latt SA, Wohlleb JC. Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes. Chromosoma 1975;52:297–316.
  • Maiti S, Chaudhury NK, Chowdhury S. Hoechst 33258 binds to G-quadruplex in the promoter region of human c-myc. Biochem Biophys Res Commun 2003;310:505–512.
  • Adhikary A, Buschmann V, Muller C, Sauer M. Ensemble and single-molecule fluorescence spectroscopic study of the binding modes of the bis-benzimidazole derivative Hoechst 33258 with DNA. Nucleic Acids Res 2003;31:2178–2186.
  • Balaban RS, Nemoto S, Finkel T. Mitochondria, oxidants, and aging. Cell 2005;120:483–495.
  • Singh SV, Srivastava SK, Choi S, Lew KL, Antosiewicz J, Xiao D, Zeng Y, Watkins SC, Johnson CS, Trump DL, Lee YJ, Xiao H, Herman-Antosiewicz A. Sulforaphane-induced cell death in human prostate cancer cells is initiated by reactive oxygen species. J Biol Chem 2005;280:19911–19924.
  • Kim GJ, Chandrasekaran K, Morgan WF. Mitochondrial dysfunction, persistently elevated levels of reactive oxygen species and radiation-induced genomic instability: a review. Mutagenesis 2006;21:361–367.
  • Ling YH, Liebes L, Zou Y, Perez-Soler R. Reactive oxygen species generation and mitochondrial dysfunction in the apoptotic response to Bortezomib, a novel proteasome inhibitor, in human H460 non-small cell lung cancer cells. J Biol Chem 2003;278:33714–33723.
  • Burdon RH. Superoxide and hydrogen peroxide in relation to mammalian cell proliferation. Free Radic Biol Med 1995;18: 775–794.
  • Hwang A, Muschel RJ. Radiation and the G 2 phase of the cell cycle. Radiat Res 1998;150:S52–S59.
  • Singh SV, Herman-Antosiewicz A, Singh AV, Lew KL, Srivastava SK, Kamath R, Brown KD, Zhang L, Baskaran R. Sulforaphane-induced G2/M phase cell cycle arrest involves checkpoint kinase 2-mediated phosphorylation of cell division cycle 25C. J Biol Chem 2004;279:25813–25822.
  • Hendry JH, West CM. Apoptosis and mitotic cell death: their relative contributions to normal-tissue and tumour radiation response. Int J Radiat Biol 1997;71:709–719.
  • Akudugu JM, Bohm L. Micronuclei and apoptosis in glioma and neuroblastoma cell lines and role of other lesions in the reconstruction of cellular radiosensitivity. Radiat Environ Biophys 2001;40:295–300.
  • Meyn RE, Stephens LC, Ang KK, Hunter NR, Brock WA, Milas L, Peters LJ. Heterogeneity in the development of apoptosis in irradiated murine tumours of different histologies. Int J Radiat Biol 1993;64:583–591.
  • Held KD. Radiation-induced apoptosis and its relationship to loss of clonogenic survival. Apoptosis 1997;2:265–282.
  • Guo G, Yan-Sanders Y, Lyn-Cook BD, Wang T, Tamae D, Ogi J, Khaletskiy A, Li Z, Weydert C, Longmate JA, Huang TT, Spitz DR, Oberley LW, Li JJ. Manganese superoxide dismutase-mediated gene expression in radiation-induced adaptive responses. Mol Cell Biol 2003;23:2362–2378.
  • Summers RW, Maves BV, Reeves RD, Arjes LJ, Oberley LW. Irradiation increases superoxide dismutase in rat intestinal smooth muscle. Free Radic Biol Med 1989;6:261–270.
  • Oberley LW, St Clair DK, Autor AP, Oberley TD. Increase in manganese superoxide dismutase activity in the mouse heart after X-irradiation. Arch Biochem Biophys 1987;254: 69–80.
  • Hussain SP, Amstad P, He P, Robles A, Lupold S, Kaneko I, Ichimiya M, Sengupta S, Mechanic L, Okamura S, Hofseth LJ, Moake M, Nagashima M, Forrester KS, Harris CC. p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis. Cancer Res 2004; 64:2350–2356.
  • Sharma PK, Bhardwaj R, Dwarakanath BS, Varshney R. Metabolic oxidative stress induced by a combination of 2-DG and 6-AN enhances radiation damage selectively in malignant cells via non-coordinated expression of antioxidant enzymes. Cancer Lett (In Press).

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