35
Views
26
CrossRef citations to date
0
Altmetric
Original Article

pKa Values and Partition Coefficients of Nitroxide Spin Probes for Membrane Bioenergetics Measurements

, , , &
Pages 315-323 | Received 28 Nov 1989, Published online: 07 Jul 2009

References

  • Ramos S., Schuldiner S., Kaback H. R. The electro-chemical gradient of protons and its relation to active transport in Escherichia coli membrane vesicles. Proceedings of the National Academy of Sciences of the United States of America 1976; 73: 1892–1896
  • Thomas J. A., Buchsbaum R. N., Zimniak A., Racker E. Intracellular pH measurements in Ehrlich ascites tumor cells utilizing spectroscopic probes generated in situ. Biochemistry. 1979; 18: 2210–2218
  • Mehlhorn R. J., Candau P., Packer L. Measurements of volumes and electrochemical gradients with spin probes in membrane vesicles. Methods in Enzymology 1982; 88: 751–762
  • Belkin S., Mehlhorn R. J., Hideg K., Hankovszky O., Packer L. Reduction and Destruction Rates of Nitroxide Spin Probes. Archives of Biochemistry and Biophysics 1987; 256: 232–243
  • Hashimoto K., Angiolilo P., Rottenberg H. Membrane potential and surface potential mitochondria. Binding of a cationic spin probe. Biochimica el Biophysica Acta 1984; 764: 55–75
  • Kamo N., Takeuichi M., Hazemoto N., Kobatake Y. Light-induced ΔpH of envelope vesicles containing halorhodopsin measured by use of spin probe. Archives of Biochemistry and Biophysics 1983; 221: 514–520
  • Melandri B. A., Mehlhorn R. J., Packer L. Light-induced proton gradients and internal volumes in chromatophores of Rhodopseudomonas sphaeroides. Archives of Biochemistry and Biophysics 1984; 235: 97–101
  • Fuchs J., Nitschmann W. H., Packer L. The antipsoriatric compound anthralin influences bioenergetic parameters and redox properties of energy transducting membranes. Journal of Investigative Dermatology 1990; 94: 71–76
  • Quintanilha A. T., Mehlhorn R. J. pH gradients across thylakoids membranes measured with a spin-labeled amine. FEBS Utters 1978; 91: 104–108
  • Belkin S., Mehlhorn R. J., Packer L. Proton gradients in intact cyanobacteria. Plant Physiology 1987; 84: 25–30
  • Lomax T. L., Mehlhorn R. J., Briggs W. R. Quantitation of [14C] IAA uptake by plant membrane vesicles using ESR volume and ΔpH determinations. Proceedings of the National Academy of Science of the United States of America. 1985; 82: 6541–6546
  • Rottenberg H. The measurement of membrane potential and ΔpH in cells, organelles and vesicles. Methods in Enzymology 1979; 55: 547–569
  • Peschek G. A., Nitschmann W. H., Czerny T. Respiratory proton extrusion and plasma membrane energization. Methods in Enzymology 1988; 167: 361–379
  • Hideg K., Lex L. Synthesis of new 2-mono and 2,5-difunctionalized pyrrolidin-1-oxyl spin labels. Journal of the Chemical Society, Perkin Transactions 1987; I: 1117–1121
  • Hideg K., Hankovszky H. O., Lex L., Kulcsár Gy. Nitroxyls; VI. Synthesis and reactions of 3-hydroxymethyl-2,2,5,5-tetramethyl-2,5-dihydropyrrole-1-oxyl and 3-formyl derivatives. Synthesis 1980; 911–914
  • Keana J. F.W., Hideg K., Birrell B. G., Hankovszky H. O., Ferguson G., Parvez M. New mono- and difunctionalized 2,2,5,5- tetramethylpyrrolidine- and Δ3-pyrroline-1-oxyl nitroxide spin-labels. Canadian Journal of Chemistry 1982; 60: 1439–1447
  • öCsek J., Hankovszky H. O., Hideg K. Synthesis of novel, highly reactive 1-oxyl-2,2,6,6-tetramethyl-1,2,5,6-tetrahydropyridine derivatives. Canadian Journal of Chemistry 1985; 63: 940–943
  • Ullman E. F., Call L., Osiecki J. H. Stable free Radicals. VIII. New imino, amidino, and carbamoyl nitroxides. Journal of Organic Chemistry 1970; 35: 3623–3631
  • Keana J. F.W., Acarregui M. J., Boyle S. L.M. 2,2-disubstituted-4,4-dimethylimidazolidinyl-3-oxy nitroxides: Indicators of aqueous acidity through variation of aN with pH. Journal of the American Chemical Society 1982; 104: 827–830
  • Fuchs J., Mehlhorn R. J., Packer L. Free radical reduction mechanisms in mouse epidermis skin homogenates. The Journal of Investigative Dermatology 1989; 93: 633–640
  • Zimmer G., Keith A. D., Packer L. Effect of sucrose and uncouplers on lipid spin labelling of mitochondria. Archives of Biochemistry and Biophysics 1972; 152: 105–113
  • Todd A. P., Mehlhorn R. J., Macey R. I. Amine and carboxylate spin probe permeability in red cells. Journal of Membrane Biology 1989; 109: 41–52
  • Todd A. P., Mehlhorn R. J., Macey R. I. Amine spin probe permeability in sonicated liposomes. Journal of Membrane Biology 1989; 109: 53–64
  • Holian A., Wilson D. F. Relationship of transmembrane pH and electrical gradients with respiration and adenosine-5′-triphosphate synthesis in mitochondria. Biochemistry 1980; 19: 4213–4221
  • Jung D. W., Davis M. H., Brierley G. P. Estimation of the pH gradient and Donnan potential in de-energized heart mitochondria. Archives of Biochemistry and Biophysics 1988; 263: 19–28
  • Falkner G., Horner F., Werdan K., Heldt H. W. pH changes in the cytoplasm of the blue-green alga Anacystis nidulans caused by light-dependent proton flux into the thylakoid space. Plant Physiology 1976; 58: 717–718
  • Boussiba S., Dilling W., Gibson J. Methylammonium transport in Anacystis nidulans R-2. Journal of Bacteriology 1984; 160: 204–210

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.