References
- Ahsan MN, Watabe S. 2001. Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus. J Protein Chem 20:4958.
- Bilkova Z, Castagna A, Zanusso G, Farinazzo A. 2005. Immunoaffinity reactors for prion protein qualitative analysis. Proteomics 53:639–647.
- Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248254.
- Calleri E, Temporini C, Perani E, Stella C, Rudaz S, Lubda D, Mellerio G, Veuthey JL, Caccialanza G, Massolini G. 2004. Development of a bioreactor based on trypsin immobilized on monolithic support for the on-line digestion and identification of proteins. J Chromatogr A 1045(1–2):99109.
- Chang, TMS. 1971. Stabilization of enzyme by microencapsulation with a concentrated protein solution or by crosslinking with glutaraldehyde. Biochem Biophys Res Com 44(6):1531–1533.
- Fengna X, Jianmin W, Zhishen J, Xianfu L. 2004. Preparation and characterization of trypsin immobilized on silica gel supported macroporous chitosan bead. Process Biochem 40:2833–2840.
- Fernandez L, Gomez L, Ramirez HL, Villalonga ML, Villalonga R. 2005. Thermal stabilization of trypsin with glycol chitosan. J Mol Catal B-Enzym 34:14–17.
- Freije JR, Mulder PP, Werkman W, Rieux L, Niederlander HA, Verpoorte E, Bischoff R. 2005. Chemically modified, immobilized trypsin reactor with improved digestion efficiency. J Proteome Res 4:1805.
- Guibault G. 1984. Analytical Uses of Immobilized Enzymes: Mod Mg Anal Chem II. New York: Marcel Dekker Inc.
- Guyonnet V, Tluscik F, Long PL, Polanowski A, Travis J. 1999. Purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin and elastase from the chicken. J Chromatogr A 852(1):217–225.
- Hunyadi-Gulyas E, Medzihradszky KF. 2004. Factors that contribute to the complexity of protein digests. Drug Discov Today 3:3–10.
- Jones JB. 1986. Enzymes in organic synthesis. Tetrahedron 42:3351.Karty JA, Ireland MM, Brun YV, Reilly JP. 2002. Artifacts and unassigned masses encountered in peptide mass mapping. J Chromatography B 782:363–383.
- Kishimura H, Hayashi K. 2002. Isolation and characteristics of trypsin from pyloric ceca of the starfish Asterina pectinifera. Comp Biochem Physiol 132:485490.
- Krogh TN, Berg T, Hojrup P. 1999. Protein analysis using enzymes immobilized to paramagnetic beads. Anal Biochem 274:153–162.
- Li Y, Xu X, Deng C, Yang P, Zhang X. 2007. Immobilization of trypsin on superparamagnetic nanoparticles for rapid and effective proteolysis. J Proteome Res 6:3849.
- Lundell N, Schreitmuller T. 1999. Sample preparation for peptide mapping: A pharmaceutical quality-control perspective. Anal Biochem 266:31–47.
- Manta C, Ferraz N, Betancor L, Antunes G, Batista-Viera F, Carlsson J, Caldwell K. 2003. Polyethylene glycol as a spacer for solid-phase enzyme immobilization. Enzyme Microb Tech 33:890–898.
- Massolini G, Calleri EJ. 2005. Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications. Separ Sci 28:7–21.
- Montero FMF, Silva GMM, Silva JBR. 2007. Immobilization of trypsin on polysaccharide film from Anacardium occ̅dentale L. and its application as cutaneous dressing. Process Biochem 42:884–888.
- Ota S, Miyazaki S, Matsuoka H, Morisato K, Shintani Y, Nakanishi K. 2007. High-throughput protein digestion by trypsin-immobilized monolithic silica with pipette-tip formula. J Biochem Bioph Meth 70:57–62.
- Podgornik A, Tennikova TB. 2002. Chromatographic reactors based on biological activity. Adv Biochem Eng/Biot 76:165–210.
- Quadroni M, James P. 1999. Proteomics and automation. Electrophoresis 20:664–677.
- Rodríguez-Martínez JA, Solá RJ, Castillo B, Cintrón-Colón HR, Rivera-Rivera I, Barletta G, Griebenow K. 2008. Stabilization of alpha chymotrypsin upon PEGylation correlates with reduced structural dynamics. Biotechnol Bioeng 101:1142–1149.
- Schaefer H, Chamrad DC, Marcus K, Reidegeld KA, Bluggel M, Meyer HE. 2005. Tryptic transpeptidation products observed in proteome analysis by LC-MS/MS. Proteomics 5:846–852.
- Slysz GW, Schriemer DC. 2003. On-column digestion of proteins in aqueous-organic solvents. Rapid Commun Mass Sp 17:1044–1050.
- Thelohan S, Jadaud P, Wainer IW. 1989. Immobilized enzymes as chromatographic phases for HPLC: The chromatography of free and derivatized amino acids on immobilized trypsin. Chromatographia 28:551–555.
- Vasudevan PT, López-Cortés N, Caswell H, Reyes-Duarte D, Plou FJ, Ballesteros A, Como K, Thomson T. 2004. A novel hydrophilic support, CoFoam, for enzyme immobilization. Biotechnol Lett 26:473–477.
- Yang Z, Domach M, Auger R, Yang FX, Russell A. 1996. Polyethylene glycol-induced stabilization of subtilisin. Enzyme Microb Tech 18:1882–1889.
- Yuhong W, You-Lo H. 2004. Enzyme immobilization to ultra-fine cellulose fibers via amphiphilic polyethylene glycol spacers. J Polym Sci A1 42:4289–4299.