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Research Article

A Fluorescent Reporter Assay for the Detection of Ligands Acting Through GI Protein-Coupled Receptors

, , , &
Pages 189-210 | Published online: 26 Sep 2008

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  • , Abbreviations used: βla, β-lactamase; GPCR, G protein-coupled receptor; NFAT, nuclear factor of activated transcription; CRE, cAMP response element; PKC, protein kinase C; CHO cells, Chinese Hamster Ovary cells; μ-OPR, μ-opioid receptor; 5HTlaR, 5-hydroxytryptamine receptor 1A; M2R, metabotropic M2 muscarinic receptor; fMLPR, formyl-methionyl peptide receptor; mGluR, metabotropic glutamate receptor; D2R, D2 dopamine receptor; 5-HT, 5-hydroxytryptamine; DPAT, 8-Hydroxydipropylaminotetralin; DP-5-CT, N, N, Dipropyl-5-carboxamidotryptamine; PMA, Phorbol 12-myristate 13-acetate; DAMGO, D-Ala2, N-Me-Phe4, Gly-ol5]-Enkephalin; β-FNA, β-Funaltrexamine; CTOP, D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Phe-Thr-NH2; S(-)-UH301, S(-)-5-fluoro-8-hydroxy-DPAT hydrochloride; NAN-190, l-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine; S(-)-Pindolol, S(-)-1 -(1 H-Indol-4-yloxy)-3-[(1 -methylethyl)amino]-2-propanol; Spiperone, 8-[4-(4-Fluorophenyl)-4-oxobutyl]-l-phenyl-1, 3, 8-triazaspiro [4, 5]decan-4-one; PTX, pertussis toxin
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