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Real-time detection of G protein activation using monomolecular Gγ FRET sensors

Real-time detection of G protein activation using monomolecular Gγ FRET sensors

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Pages 63-72 | Received 27 Sep 2012, Accepted 13 Dec 2012, Published online: 22 Jan 2013

References

  • Filmore D. It’s a GPCR world. Mod Drug Discov (Am Chem Soc) 2004;November:24–8
  • Overington JP, Al-Lazikani B, Hopkins AL. How many drug targets are there? Nat Rev Drug Discov 2006;5:993–6
  • Gilman AG. G proteins: transducers of receptor-generated signals. Annu Rev Biochem 1987;56:615–49
  • Simon MI, Strathmann MP, Gautam N. Diversity of G proteins in signal transduction. Science 1991;252:802–8
  • Neves SR, Ram PT, Iyengar R. G protein pathways. Science 2002;296:1636–9
  • Cabrera-Vera TM, Vanhauwe J, Thomas TO, et al. Insights into G protein structure, function and regulation. Endocr Rev 2003;24:765–81
  • Digby GJ, Lober RM, Sethi PR, Lambert NA. Some G protein heterotrimers physically dissociate in living cells. Proc Natl Acad Sci USA 2006;103:17789–94
  • Akgoz M, Kalyanaraman V, Gautam N. Receptor-mediated reversible translocation of the G protein betagamma complex from the plasma membrane to the Golgi complex. J Biol Chem 2004;279:51541–4
  • Akgoz M, Kalyanaraman V, Gautam N. G protein betagamma complex translocation from plasma membrane to Golgi complex is influenced by receptor gamma subunit interaction. Cell Signal 2006;18:1758–68
  • DeWire SM, Ahn S, Lefkowitz RJ, Shenoy SK. Beta-arrestins and cell signaling. Annu Rev Physiol 2007;69:483–510
  • Pitcher JA, Freedman NJ, Lefkowitz RJ. G protein-coupled receptor kinases. Annu Rev Biochem 1998;67:653–92
  • Vilardaga JP, Bunemann M, Krasel C, et al. Measurement of the millisecond activation switch of G protein-coupled receptors in living cells. Nat Biotechnol 2003;21:807–12
  • Chachisvilis M, Zhang YL, Frangos JA. G protein-coupled receptors sense fluid shear stress in endothelial cells. Proc Natl Acad Sci USA 2006;103:15463–8
  • Zhang YL, Frangos JA, Chachisvilis M. Mechanical stimulus alters conformation of type 1 parathyroid hormone receptor in bone cells. Am J Physiol Cell Physiol 2009;296:C1391–9
  • Candelario J, Chachisvilis M. Mechanical stress stimulates conformational changes in 5-hydroxytryptamine receptor 1B in bone cells. Cell Mol Bioeng 2012;5:277–86
  • Takeda S, Kadowaki S, Haga T, et al. Identification of G protein-coupled receptor genes from the human genome sequence. FEBS Lett 2002;520:97–101
  • Janetopoulos C, Jin T, Devreotes P. Receptor-mediated activation of heterotrimeric G-proteins in living cells. Science 2001;291:2408–11
  • Bunemann M, Frank M, Lohse MJ. Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci USA 2003;100:16077–82
  • Chisari M, Saini DK, Cho JH, et al. G protein subunit dissociation and translocation regulate cellular response to receptor stimulation. PLoS One 2009;4:e7797
  • Miyawaki A. Development of probes for cellular functions using fluorescent proteins and fluorescence resonance energy transfer. Annu Rev Biochem 2011;80:357–73
  • Miyawaki A. Visualization of the spatial and temporal dynamics of intracellular signaling. Dev Cell 2003;4:295–305
  • Violin JD, Zhang J, Tsien RY, Newton AC. A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C. J Cell Biol 2003;161:899–909
  • Saini DK, Kalyanaraman V, Chisari M, Gautam N. A family of G protein betagamma subunits translocate reversibly from the plasma membrane to endomembranes on receptor activation. J Biol Chem 2007;282:24099–108
  • Rizzo MA, Springer GH, Granada B, Piston DW. An improved cyan fluorescent protein variant useful for FRET. Nat Biotechnol 2004;22:445–9
  • Griesbeck O, Baird GS, Campbell RE, et al. Reducing the environmental sensitivity of yellow fluorescent protein. Mechanism and applications. J Biol Chem 2001;276:29188–94
  • Muntz KH, Sternweis PC, Gilman AG, Mumby SM. Influence of gamma subunit prenylation on association of guanine nucleotide-binding regulatory proteins with membranes. Mol Biol Cell 1992;3:49–61
  • Azpiazu I, Akgoz M, Kalyanaraman V, Gautam N. G protein betagamma11 complex translocation is induced by Gi, Gq and Gs coupling receptors and is regulated by the alpha subunit type. Cell Signal 2006;18:1190–200
  • Wittinghofer A. Signaling mechanistics: aluminum fluoride for molecule of the year. Curr Biol 1997;7:R682–5
  • Bigay J, Deterre P, Pfister C, Chabre M. Fluoroaluminates activate transducin-GDP by mimicking the gamma-phosphate of GTP in its binding site. FEBS Lett 1985;191:181–5
  • Burns DL. Subunit structure and enzymic activity of pertussis toxin. Microbiol Sci 1988;5:285–7
  • Abou-Samra AB, Juppner H, Force T, et al. Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium. Proc Natl Acad Sci USA 1992;89:2732–6
  • Pines M, Fukayama S, Costas K, et al. Inositol 1-,4-,5-trisphosphate-dependent Ca2+ signaling by the recombinant human PTH/PTHrP receptor stably expressed in a human kidney cell line. Bone 1996;18:381–9
  • Gudi S, Nolan JP, Frangos JA. Modulation of GTPase activity of G proteins by fluid shear stress and phospholipid composition. Proc Natl Acad Sci USA 1998;95:2515–9
  • Zou Y, Akazawa H, Qin Y, et al. Mechanical stress activates angiotensin II type 1 receptor without the involvement of angiotensin II. Nat Cell Biol 2004;6:499–506
  • Nguyen AW, Daugherty PS. Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat Biotechnol 2005;23:355–60
  • Myung CS, Lim WK, DeFilippo JM, et al. Regions in the G protein gamma subunit important for interaction with receptors and effectors. Mol Pharmacol 2006;69:877–87
  • Borjigin J, Nathans J. Insertional mutagenesis as a probe of rhodopsin’s topography, stability and activity. J Biol Chem 1994;269:14715–22
  • Bergaya S, Meneton P, Bloch-Faure M, et al. Decreased flow-dependent dilation in carotid arteries of tissue kallikrein-knockout mice. Circ Res 2001;88:593–9
  • Tu X, Pellegrini G, Galli C, et al. PTH receptor 1 expression in osteocytes is indispensible for the anabolic effect of mechanical loading in mice. American Society for Bone and Mineral Research Annual Meeting; 2011 September 17; San Diego; 2011:Abstract 1067

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