8
Views
14
CrossRef citations to date
0
Altmetric
Research Article

Factors Affecting the Distribution and Stability of Unoccupied 1,25-Dihydroxyvitamin D3 Receptors

, , &
Pages 331-346 | Published online: 26 Sep 2008

References

  • , Abbreviations: 1,25(OH)2D3 = 1,25-dihydroxyvitamin D3; TED buffer = 10 mM Tris, 1.5 mM EDTA, 1.0 mM dithiothreitol; KTED = TED buffer + 0.3 M KCl; TD = 10 mM Tris + 1.0 mM dithiothreitol; TPCK = L-1-Tosylamide-2-phenylethyl-chloromethyl ketone; TLCK = N-α-p-Tosyl-L-lysine Chloromethyl ketone; STKM = 0.25 M sucrose, 50 mM Tris, 25 mM KCl, 5 mM MgCl2; PMSF = phenylmethylsulfonyl fluoride
  • Norman A. W. Vitamin D: The Calcium Homeostatic Hormone. Academic Press, New York 1979
  • Walters M. R., Hunziker W., Norman A. W. Unoccupied 1,25-dihydroxyvitamin D receptors: nuclear/cytosol ratio depends on ionic strength. J. Biol. Chem. 1980; 255: 6799–6805
  • Walters M. R., Hunziker W., Norman A. W. Cytosol preparations are inadequate for quantitating unoccupied receptors for 1,25-dihydroxyvitamin D. J. Receptor Res. 1980; 1: 313–327
  • Kream B. E., Yamada S., Schnoes H. K., DeLuca H. F. Specific cytosol-binding protein for 1,25-dihydroxyvitamin D3 in rat intestine. J. Biol. Chem. 1977; 252: 4501–4505
  • Feldman D., McCain T. A., Hirst M. A., Chen T. L., Colston K. W. Characterization of a cytoplasmic receptor-like binder for 1,25-dihydroxycholecalciferol in rat intestinal mucosa. J. Biol. Chem. 1979; 254: 10378–10384
  • Wecksler W. R., Ross F. P., Mason R. S., Posen S., Norman A. W. Studies on the mode of action of calciferol XXIV. Biochemical properties of the 1,25-dihydroxyvitamin D3 cytoplasmic receptors from chick and human parathyroid glands, Arch. Biochem. Biophys. 1980; 201: 95–103
  • Wecksler W. R., Ross F. P., Mason R. S., Norman A. W. Studies on the Mode of Action of Calciferol XXI. Biochemical properties of the lα,25-dihydroxyvitamin D3 cytosol receptors from human and chicken intestinal mucosa. J. Clin. Endocrin. Metab. 1980; 50: 152–157
  • Wecksler W. R., Norman A. W. Biochemical properties of lα,25-dihydroxyvitamin D receptors. J. Steroid Biochem. 1980; 13: 977–989
  • Hunziker W., Walters M. R., Norman A. W. 1,25-dihydroxyvitamin D receptors; differential quantitation of endogenously occupied and unoccupied sites. J. Biol. Chem. 1980; 255: 9534–9537
  • Walters M. R., Hunziker W., Norman A. W. Apparent nuclear localization of unoccupied receptors for 1,25-dihydroxyvitamin D3, Biochem. Biophys. Res. Commun. 1981; 98: 990–996
  • Scatchard G. ; The attractions of proteins for small molecules. Ann. N.Y. Acad. Sci. 1949; 51: 660–671
  • “Transformation” herein will denote an increase in DNA/chromatin affinity: “activation” will denote increased ligand binding as per Leach, et al. (16)
  • , Trasylol = aprotinin, bovine trypsin kallikrein inhibitor; available from Mobay Chemical Corp
  • Hunziker W., Walters M. R., Bishop J. E., Norman A. W. Differential effects of protease inhibitors on 1,25-dihydroxyvitamin D3 receptors, (In Preparation)
  • Leach K. L., Dahmer M. K., Hammond N. D., Sando J. J., Pratt W. B. Molybdate inhibition of glucocorticoid receptor inactivation and transformation. J. Biol. Chem. 1979; 254: 11884–11890
  • Nielsen C. J., Sando J. J., Vogel W. M., Pratt W. B. Glucocorticoid receptor inactivation under cell-free conditions. J. Biol. Chem. 1977; 252: 7568–7578
  • Nishigori H., Toft D. Inhibition of progesterone receptor inactivation by sodium molybdate. Biochemistry 1980; 19: 77–83
  • Franceschi R. T., DeLuca H. F. Aggregation properties of the 1,25-dihydroxyvitamin D3 receptor from chick intestinal cytosol. J. Biol. Chem. 1979; 254: 11629–11635
  • Baker M. E., Fanestil D. D. Effect of protease inhibitors and substrates on deoxycorticosterone binding to its receptor in dog MDCK kidney cells. Nature 1977; 269: 810–812
  • Baker M. E., Fanestil D. D. Diethylpyrocarbonate inhibition of estrogen binding to rat alpha-fetoprotein: evidence that one or more histidine residues regulate estrogen binding, Biochem. Biophys. Res. Commun. 1981; 98: 976–982
  • , This dose has been optimized for TED-chromatin of chick intestinal mucosa. In less pure systems, this effect may be masked due to competition for TPCK by other proteins - ex. KTED cytosol of the chick pancreas (23)
  • Christakos S., Norman A. W. Biochemical characterization of 1,25-dihydroxyvitamin D3 receptors in chick pancreas and kidney. Endocrinology 1981; 108: 140–149
  • Bar A., Hunziker W., Walters M. R., Bishop J. E., Norman A. W. Studies on the mode of action of calciferol XXXIV. Relationship between plasma 1,25-dihydroxyvitamin D3 and its intestinal receptors and calcium binding protein levels as influenced by dietary calcium or phosphorus restriction, (In Preparation)
  • Brumbaugh P. F., Haussler M. R. 1,25-dihydroxychole-calciferol receptors in intestine, II. Temperature dependent transfer of the hormone to chromatin via a specific cytosol receptor. J. Biol. Chem. 1974; 249: 1258–1262
  • Puca G. A., Nola E., Sica V., Bresciani F. Estrogen binding proteins of calf uterus; molecular and functional characterization of the receptor transforming factor; A Ca2+- activated protease. J. Biol. Chem. 1977; 252: 1358–1366
  • Vedeckis W. V., Freeman M. R., Schrader W. T., O'Malley B. W. Progesterone-binding components of chick oviduct; partial purification and characterization of a calcium-activated protease which hydrolyzes the progesterone receptor. Biochemistry 1980; 19: 335–343
  • Carter D. B., Efird P. H., Chae C -B. Chromatin-bound protease; [3H]diisopropyl fluorophosphate labeling patterns of chromatin. Biochemistry 1976; 15: 2603–2607
  • Pantazis P., Sakamoto M. Thermostability of chromatin and variations in chromosomal proteins isolated under different ionic conditions. J. Biochem. 1980; 88: 1283–1289

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.