References
- , Abbreviations: 1,25(OH)2D3 = 1,25-dihydroxyvitamin D3; TED buffer = 10 mM Tris, 1.5 mM EDTA, 1.0 mM dithiothreitol; KTED = TED buffer + 0.3 M KCl; TD = 10 mM Tris + 1.0 mM dithiothreitol; TPCK = L-1-Tosylamide-2-phenylethyl-chloromethyl ketone; TLCK = N-α-p-Tosyl-L-lysine Chloromethyl ketone; STKM = 0.25 M sucrose, 50 mM Tris, 25 mM KCl, 5 mM MgCl2; PMSF = phenylmethylsulfonyl fluoride
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- “Transformation” herein will denote an increase in DNA/chromatin affinity: “activation” will denote increased ligand binding as per Leach, et al. (16)
- , Trasylol = aprotinin, bovine trypsin kallikrein inhibitor; available from Mobay Chemical Corp
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- , This dose has been optimized for TED-chromatin of chick intestinal mucosa. In less pure systems, this effect may be masked due to competition for TPCK by other proteins - ex. KTED cytosol of the chick pancreas (23)
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