A System for the Translation of Receptor Messenger-RNA and the Study of the Assembly of Functional Receptors

References

• Sumikawa K., Houghton M., Smith J. C., Bell L., Richards B. M., Barnard E. A. The molecular cloning and characterisation of cDNA coding for the α-subunit of the acetylcholine receptor. Nucleic Acids Res. 1982; 10: 5809
   PubMed Web of Science ®Google Scholar
• Noda M., Takahashi H., Tanabe T., Toyosato M., Furutani Y., Hirose T., Asai M., Inayama S., Miyata T., Numa S. Primary structure of α-subunit precursor of Torpedocalifornica acetylcholine receptor deduced from cDNA sequence. Nature 1982; 299: 793
   PubMed Web of Science ®Google Scholar
• Noda M., Takahashi H., Tanabe T., Toyasato M., Kikyotani S., Furutani Y., Hirose T., Takashima H., Inayama S., Niyata T., Numa S. Structural homology of Torpedocalifornica acetylcholine receptor subunits. Nature 1983; 302: 528
   PubMed Web of Science ®Google Scholar
• Claudio T., Ballivet M., Patrick J., Heinemann S. Nucleotide and deduced amino acid sequences of Torpedocalifornica acetylcholine receptor α-subunit. Proc. Natl. Acad. Sci. USA 1983; 80: 1111
   PubMed Web of Science ®Google Scholar
• Devillers-Thiery A., Giraudat J., Bentaboulet M., Changeux J.-P. Complete mRNA coding sequence of the acetylcholine binding α-subunit of Torpedomarmorata acetylcholine receptor: A model for the transmembrane organization of the polypeptide chain. Proc. Natl. Acad. Sci. USA 1983; 80: 2067
   PubMed Web of Science ®Google Scholar
• Noda M., Furutani Y., Takahashi H., Toyosato Tanabe M., Shimizu S., Kikyotani S., Kayano T., Hirose T., Inayama S., Numa S. Cloning and sequence analysis of calf cDNA and human genomic DNA encoding α-subunit precursor of muscle acetylcholine receptor. Nature 1983; 305: 818
   PubMed Web of Science ®Google Scholar
• Gurdon J. B., Lane C. D., Woodland H. R., Marbaix G. Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells. Nature 1971; 233: 177
   PubMed Web of Science ®Google Scholar
• Gurdon J. B. The control of gene expression in animal development. Clarendon, Oxford 1974
   Google Scholar
• Lane C. D., Shannon S., Craig R. Sequestration and turnover of guinea-pig milk proteins and chicken ovalbumin in Xenopus oocytes. Eur. J. Biochem. 1979; 101: 485
   PubMedGoogle Scholar
• Sumikawa K., Houghton M., Emtage J. S., Richards B. M., Barnard E. A. Active multi-subunit ACh receptor assembled by translation of heterologous mRNA in Xenopus oocytes. Nature 1981; 292: 862
   PubMed Web of Science ®Google Scholar
• Mendez B., Valenzuela P., Martial J. A., Baxter J. D. Cell-free synthesis of acetylcholine receptor polypeptides. Science 1980; 209: 695
   PubMed Web of Science ®Google Scholar
• Anderson D. J., Blobel G. Invitro glycosylation and membrane insertion of the four subunits of Torpedo acetylcholine receptor. Proc. Natl. Acad. Sci. USA 1981; 78: 5598
   PubMed Web of Science ®Google Scholar
• Anderson D. J., Blobel G. Molecular events in the synthesis and assembly of a nicotinic acetylcholine receptor. Cold Spring Harbor Symp. Quant. Biol. 1983; 48: 125
   PubMedGoogle Scholar
• Chirgwin J. M., Przybla A.-E., MacDonald R. J., Rutter W. J. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 1979; 18: 5294
   PubMed Web of Science ®Google Scholar
• Smart T. G., Constanti A., Bilbe G., Brown D. A., Barnard E. A. Synthesis of functional chick brain GABA-benzodiazepine-barbiturate receptor complexes in mRNA-injected Xenopus oocytes. Neurosci. Lett. 1983; 40: 55
   PubMed Web of Science ®Google Scholar
• Barnard E. A., Miledi R., Sumikawa K. Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes. Proc. R. Soc. Lond. B 1982; 125: 241
   Google Scholar
• Barnard E. A., Beeson D., Bilbe G., Brown D. A., Conti-Tronconi B. M., Dolly J. O., Dunn S. M.J., Mehraban F., Richards B. M., Smart T. G. Acetylcholine and GABA receptors: Subunits of central and peripheral receptors and their encoding nucleic acids. Cold Spring Harbor Symp. Quant. Biol. 1983; 48: 109
   PubMedGoogle Scholar
• Sumikawa K., Miledi R., Houghton Barnard M., In E. A. Cell Surface Receptors, P. G. Strange. MacMillan and Co., London 1983; 249
   Google Scholar
• Miledi R., Parker I., Sumikawa K. Properties of acetylcholine receptors translated by cat muscle mRNA in Xenopus ooctes. EMBO J. 1982; 1: 1307
   PubMed Web of Science ®Google Scholar
• Vandlen R. L., Wu W. C.-S., Esenach J. C., Raftery M. A. Studies of the composition of purified Torpedocalifornica acetylcholine receptor and its subunits. Biochemistry 1979; 18: 1845
   PubMed Web of Science ®Google Scholar
• Lindstrom J., Merlie J., Yogeeswaran G. Biochemical properties of acetylcholine receptor subunits from Torpedo californica. Biochemistry 1979; 18: 4465
   PubMed Web of Science ®Google Scholar
• Sumikawa K., Barnard E. A., unpublished results
   Google Scholar
• Sigel E., Stephenson F. A., Mamalaki C., Barnard E. A. A γ-aminobutyric acid/benzodiazepine receptor complex of bovine cerebral cortex. Purification and partial characterization. J. Biol. Chem. 1983; 258: 6965
   PubMed Web of Science ®Google Scholar
• Sigel E., Barnard E. A. A γ-aminobutyric acid/benzodiazepine receptor complex from bovine cerebral cortex. Improved purification with preservation of regulatory sites and their interactions. J. Biol. Chem. 1984; 259, (in press)
   Web of Science ®Google Scholar
• Houamed K., Bilbe G., Smart T. G., Constanti A., Brown D. A., Barnard E. A., Richards B. M. Expression of functional GABA, glycine and glutamate receptors in Xenopus oocytes injected with rat brain mRNA. Nature 1984, (in press)
   PubMed Web of Science ®Google Scholar
• Barker J. A., Dufy B., Owen D. G., Segal M. Excitable membrane properties of cultured central nervous system neurons and clonal pituitary cells. Cold Spring Harbour Symp. Quant. Biol. 1983; 48: 259
   Google Scholar
• Gunderson C. B., Miledi R., Parker I. Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes. Proc. R. Soc. Lond. B 1983; 219: 103
   PubMed Web of Science ®Google Scholar
• Fairclough R. H., Finer-Moore J., Love R. A., Kristofferson D., Desmeules P. J., Stroud R. M. Subunit organization and structure of an acetylcholine receptor. Cold Spring Harbour Symp. Quant. Biol. 1983; 48: 9
   Google Scholar
• Mishina M., Kurosati T., Tobimatsu T., Morimoto Y., Noda M., Yamamoto T., Terao M., Lindstrom J., Takahashi T., Kuno M., Numa S. Expression of functional acetylcholine receptor from cloned cDNAs. Nature 1984; 307: 604
   PubMed Web of Science ®Google Scholar
• Blobel G. Intracellular protein topogenesis. Proc. Natl. Acad. Sci. USA 1980; 77: 1496
   PubMed Web of Science ®Google Scholar