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Research Article

The Role of β, γ-Subunits of Guanine Nucleotide Binding Proteins in Control of a Reconstituted Signal Transmission Chain Containing Purified Components of the Adenylate Cyclase System

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Pages 17-42 | Published online: 26 Sep 2008

References

  • Cerione R. A., Staniszewski C., Caron M G., Lefkowitz R. J., Codina J., Birnbaumer L. A role for Ni in the hormonal stimulation of adenylate cyclase. Nature 1985; 318: 293–295
  • Tanabe T., Nukada T., Nishikawa Y., Sugimoto K., Suzuki H., Takahashi H., Noda M., Haga T., Ichiyama A., Kangawa K., Minamino N., Matsuo H., Numa S. Primary structure of the α-subunit of transducin and its relationship to ras proteins. Nature 1985; 315: 242–245
  • Robishaw J. D., Russell D. W., Harris B. A., Smigel M. D., Gilman A. G. Deduced primary structure of the α subunit of the GTP-binding stimulatory protein of adenylate cyclase, Proc. Natl. Acad. Sci. USA 1986; 83: 1251–1255
  • Nukada T., Tanabe T., Takahashi H., Noda M., Haga K., Haga T., Ichiyama A., Kangawa K., Hiranaga M., Matsuo H., Numa S. Primary structure of the α-subunit of bovine adenylate cyclase-inhibiting G-protein deduced from the cDNA sequence. FEBS Lett. 1986; 197: 305–310
  • Manning D. R., Gilman A. G. The regulatory components of adenylate cyclase and transducin. J. Biol. Chem. 1983; 258: 7059–7063
  • Sugimoto K., Nukada T., Tanabe T., Takahashi H., Noda M., Minamino N., Kangawa K., Matsuo H., Hirose T., Inayama S., Numa S. Primary structure of the ß-subunit of bovine transducin deduced from the cDNA sequence. FEBS Lett. 1985; 191: 235–240
  • Gierschik P., Codina J., Simons C., Birnbaumer L., Spiegel A. Antisera against a guanine nucleotide binding protein from retina cross-react with the ß subunit of the adenylyl cyclase-associated guanine nucleotide binding proteins, Ns and Ni, Proc. Natl. Acad. Sci. USA 1985; 82: 727–731
  • Hildebrandt J. D., Codina J., Rosenthal W., Birnbaumer L., Neer E. J., Yamazaki A., Bitensky M. W. Characterization by two-dimensional peptide mapping of the γ subunits of Ns and Ni, the regulatory proteins of adenylyl cyclase, and of transducin, the guanine nucleotide-binding protein of rod outer segments of the eye. J. Biol. Chem. 1985; 260: 14867–14872
  • Hanski E., Sternweis P. C., Northup J. K., Dromerick A. W., Gilman A. G. The regulatory component of adenylate cyclase. J. Biol. Chem. 1981; 256: 12911–12919
  • Northup J. K., Sternweis P. C., Gilman A. G. The subunits of the stimulatory regulatory component of adenylate cyclase. J. Biol. Chem. 1983; 258: 11361–11368
  • Riemenschneider L. 1985, Dissertation Würzburg
  • Gierschik P., Falloon J., Milligan G., Pines M., Gallin J. I., Spiegel A. Immunochemical evidence for a novel pertussis toxin substrate in human neutrophils. J. Biol. Chem. 1986; 261: 8058–8062
  • Gierschik, et al, in preparation
  • Stryer L., Hurley J. B., Fung B. K.-K. Transducin: an amplifier protein in vision. TIBS 1981; 6: 245–247
  • Feder D., Im M.-J., Klein H. W., Hekman M., HolzhÖFer A., Dees C., Levitzki A., Helmreich E. J.M., Pfeuffer T. Reconstitution of ß1-adrenoceptor-dependent adenylate cyclase from purified components. EMBO J. 1986; 5: 1509–1514
  • Pfeuffer T., Metzger H. 7–0-Hemisuccinyl-deacetyl forskolin-sepharose: a novel affinity support for purification of adenylate cyclase. FEBS Lett. 1982; 146: 369–375
  • JÜRss R., Hekman M., Helmreich E. J.M. Proteolysis-associated deglycosylation of ß1-adrenergic receptor in turkey erythrocytes and membranes. Biochemistry 1985; 24: 3349–3354
  • Hekman M., Feder D., Keenan A. K., Gal A., Klein H. W., Pfeuffer T., Levitzki A., Helmreich E. J.M. Reconstitution of ß-adrenergic receptor with components of adenylate cyclase. EMBO J. 1984; 3: 3339–3345
  • Sternweis P. C., Northup J. K., Smigel M. D., Gilman A. G. The regulatory component of adenylate cyclase. J. Biol. Chem. 1981; 256: 11517–11526
  • Pfeuffer E., Mollner S., Pfeuffer T. Adenylate cyclase from bovine brain cortex: purification and characterization of the catalytic unit. EMBO J. 1985; 4: 3675–3679
  • Pedersen S. E., Ross E. M. Functional activation of ß-adrenergic receptors by thiols in the presence or absence of agonists. J. Biol. Chem. 1985; 260: 14150–14157
  • Hekman M., Schiltz E., Henis Y. I., Elson E. L., Helmreich E. J.M. Mobility, localization, and heterogeneity of ß-adrenergic receptors. Advances Cyclic Nucl. Prot. Phos. Res, P. Greengard, G. A. Robison, R. Paoletti, S. Nicosia. Raven Press, New York 1984; Vol. 17: 47–60
  • Northup J. K., Smigel M. D., Gilman A. G. The guanine nucleotide activating site of the regulatory component of adenylate cyclase. J. Biol. Chem. 1982; 257: 11416–11423
  • Brandt D. R., Ross E. M. GTPase activity of the stimulatory GTP-binding regulatory protein of adenylate cyclase, Gs. J. Biol. Chem. 1985; 260: 266–272
  • Salomon Y., Londos C., Rodbell M. A highly sensitive adenylate cyclase assay, Anal. Biochem. 1974; 58: 541–548
  • Pfeuffer E., Dreher R.-M., Metzger H., Pfeuffer T. Catalytic unit of adenylate cyclase: Purification and identification by affinity crosslinking, Proc. Natl. Acad. Sci. USA 1985; 82: 3086–3090
  • May D. C., Ross E. M., Gilman A. G., Smigel M. D. Reconstitution of catecholamine-stimulated adenylate cyclase activity using three purified proteins. J. Biol. Chem. 1985; 260: 15829–15833
  • Londos C., Salomon Y., Lin M. C., Harwood J. P., Schramm M., Wolff J., Rodbell M. 5′-Guanylylimidodiphosphate, a potent activator of adenylate cyclase systems in eukaryotic cells, Proc. Natl. Acad. Sci. USA 1974; 71: 3087–3090
  • Cerione R. A., Codina J., Kilpatrick B. F., Staniszewski C., Gierschik P., Somers R. L., Spiegel A. M., Birnbaumer L., Caron M. G., Lefkowitz R. J. Transducin and the inhibitory nucleotide regulatory protein inhibit the stimulatory nucleotide regulatory protein mediated stimulation of adenylate cyclase in phospholipid vesicle systems. Biochemistry 1985; 24: 4499–4503
  • Cerione R. A., Staniszewski C., Gierschik P., Codina J., Somers R. L., Birnbaumer L., Spiegel A. M., Caron M. G., Lefkowitz R. J. Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. J. Biol. Chem. 1986; 261: 9514–9520
  • Oakley B. R., Kirsch D. R., Morris N. R. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels, Anal. Biochem. 1980; 105: 361–363
  • Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685
  • Sternweis P. C., Robishaw J. D. Isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. J. Biol. Chem. 1984; 259: 13806–13813
  • Roof D. J., Applebury M. L., Sternweis P. C. Relationships within the family of GTP-binding proteins isolated from bovine central nervous system. J. Biol. Chem. 1985; 260: 16242–16249
  • Neer E. J., Lok J. M., Wolf L. G. Purification and properties of the inhibitory guanine nucleotide regulatory unit of brain adenylate cyclase. J. Biol. Chem. 1984; 259: 14222–14229
  • Robishaw J. D., Smigel M. D., Gilman A. G. Molecular basis for two forms of the G protein that stimulates adenylate cyclase. J. Biol. Chem. 1986; 261: 9587–9590
  • Brandt D. R., Asano T., Pedersen S. E., Ross E. M. Reconstitution of catecholamine-stimulated guanosinetriphosphatase activity. Biochemistry 1983; 22: 4357–4362
  • Katada T., Bokoch G. M., Northup J. K., Ui M., Gilman A. G. The inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. J. Biol. Chem. 1984; 259: 3568–3577
  • Codina J., Hildebrandt J. D., Birnbaumer L. Effects of guanine nucleotides and Mg on human erythrocyte Ni and Ns, the regulatory components of adenylyl cyclase. J. Biol. Chem. 1984; 259: 11408–11418
  • Katada T., Oinuma M., Ui M. Two guanine nucleotide-binding proteins in rat brain serving as the specific substrate of islet-activating protein, pertussis toxin. J. Biol. Chem. 1986; 261: 8182–8191
  • Tolkovsky A. M., Levitzki A. Mode of coupling between the β-adrenergic receptor and adenylate cyclase in turkey erythrocytes. Biochemistry 1978; 17: 3795–3810
  • Sevilla N., Steer M. L., Levitzki A. Synergistic activation of adenylate cyclase by guanylyl imidophosphate and epinephrine. Biochemistry 1976; 15: 3493–3499
  • Rosenthal W., Koesling D., Rudolph U., Kleuss C., Pallast M., Yajima M., Schultz G. Identification and characterization of the 35-kDa ß subunit of guanine-nucleotide-binding proteins by an antiserum raised against transducin, Eur. J. Biochem. 1986; 158: 255–263
  • Sternweis P. C. The purified α subunits of Go and Gi from bovine brain require ßγ for association with phospholipid vesicles. J. Biol. Chem. 1986; 261: 631–637
  • Pfeuffer T. GTP-binding proteins in membranes and the control of adenylate cyclase activity. J. Biol. Chem. 1977; 252: 7224–7234
  • Sullivan K. A., Liao Y.-C., Alborzi A., Beiderman B., Chang F.-H., Masters S. B., Levinson A. D., Bourne H. R. Inhibitory and stimulatory G proteins of adenylate cyclase: cDNA and amino acid sequences of the α chains, Proc. Natl. Acad. Sci. USA 1986; 83: 6687–6691
  • Fung B. K.-K. Characterization of transducin from bovine retinal rod outer segments. I. Separation and reconstitution of the subunits, J. Biol. Chem. 1983; 258: 10495–10502
  • Fung B. K.-K., Nash C. R. Characterization of transducin from bovine retinal rod outer segments. II. Evidence for distinct binding sites and conformational changes revealed by limited proteolysis with trypsin, J. Biol. Chem. 1983; 258: 10503–10510
  • Kanaho Y., Tsai S.-C., Adamik R., Hewlett E. L., Moss J., Vaughan M. Rhodopsin-enhanced GTPase activity of the inhibitory GTP-binding protein of adenylate cyclase. J. Biol. Chem. 1984; 259: 7378–7381
  • Abood M. E., Hurley J. B., Pappone M-C., Bourne H. R., Stryer L. Functional homology between signal-coupling proteins. J. Biol. Chem. 1982; 257: 10540–10543
  • Manning D. R., Fraser B. A., Kahn R. A., Gilman A. G. ADP-ribosylation of transducin by islet-activating protein. J. Biol. Chem. 1984; 259: 749–756
  • Lerea C. L., Somers D. E., Hurley J. B., Klock I. B., Bunt-Milam A. H. Identification of specific transducin α subunits in retinal rod and cone photoreceptors. Science 1986; 234: 77–80

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