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Journal of Receptor Research Volume 8, 1988 - Issue 1-4
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Research Article

Bioimmunoassay (BIA): A Sandwich Immunoassay Scheme Employing Monoclonal Antibodies and Hormone Receptors to Quantify Analytes

William R. Moyle Department of Obstetrics and Gynecology and Anatomy, Robert Wood Johnson (Rutgers) Medical School, Piscataway, NJ, 08854; Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032
,
Donna M. Anderson Department of Obstetrics and Gynecology and Anatomy, Robert Wood Johnson (Rutgers) Medical School, Piscataway, NJ, 08854; Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032
,
Gordon J. Macdonald Department of Obstetrics and Gynecology and Anatomy, Robert Wood Johnson (Rutgers) Medical School, Piscataway, NJ, 08854; Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032
&
E. Glenn Armstrong Department of Obstetrics and Gynecology and Anatomy, Robert Wood Johnson (Rutgers) Medical School, Piscataway, NJ, 08854; Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY, 10032
Pages 419-436 | Published online: 26 Sep 2008

References

  • Moyle W. R., Ehrlich P. H., Canfield R. E. Use of monoclonal antibodies to hCG subunits to examine the orientation of hCG in the hormone-receptor complex. Proc. Soc. Nat'l. Acad. Sci. (USA) 1982; 79: 2245–2249
  • Ehrlich P. H., Moustafa Z. A., Krichevsky A., Birken S., Armstrong E. G., Canfield R. E. Characterization and relative orientation of epitopes for monoclonal antibodies and antisera to human chorionic gonadotropin. Am. J. Reprod. Immunol. Microbiol. 1985; 8: 48–54
  • Macdonald G. J., Anderson D. M., Tinglu G., Ehrlich P. H., Moyle W. R. (1985) Orientation of human chorionic gonadotropin in ovarian-hCG-receptor complexes. Proceedings of the fifth ovarian workshop. 1985, D O Toft, R J Ryan. Ovarian Workshops Press, Champane, IL, 113–117
  • Morgan F. S., Birken S., Canfield R. E. The amino acid sequence of human chorionic gonadotropin: the alpha and beta subunit. J. Biol. Chem. 1975; 250: 5247–5258
  • Parlow A. F. A rapid bioassay method for LH and factors stimulating LH secretion. Fed Proc 1958; 17: 40
  • Lee C. Y., Ryan R. J. Luteinizing hormone receptors: specific binding of human luteinizing hormone to homogenates of luteinized rat ovaries. Proc. Nat'l. Acad. Sci. (USA) 1972; 69: 3520–3523
  • Lee C. Y., Ryan R. J. Radioreceptor assay for human chorionic gonadotropin. J. Clin. Endocrinol. Metab. 1975; 40: 228–233
  • Pierce J. G., Parsons T. F. Glycoprotein hormones: structure and function. Ann. Rev. Biochem. 1981; 50: 465–495
  • Moyle W. R., Netburn M., Cosgrove A. E., Krieger J., Bahl O. P. hCG-Leydig cell functional binding kinetics: threshold and nonlinearity in response. Am. J. Physiol. 1980; 238: E293–E302
  • Garfink J. E., Moyle W. R., Bahl O. P. A rapid sensitive bioassay for luteinizing hormone-like gonadotropins. Gen. Comp. Endocrinol. 1976; 30: 292–300
  • Licht P., Papkoff H., Farmer S. W., Muller C. H., Tsui H. W., Crews D. Evolution of gonadotropin structure and function. Rec. Prog. Hormone Res 1977; 33: 169–248
  • Moyle W. R., Bahl O. P., Marz L. Role of the carbohydrate fraction of human chorionic gonadotropin in the mechanism of hormone action. J. Biol. Chem. 1975; 250: 9163–9169
  • Manjunath P., Sairam M. R. Biochemical, biological, and immunological properties of chemically deglycosylated human chorionic gonadotropin. J. Biol. Chem. 1982; 257: 7109–7115

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