References
- King A. C., Cuatrecasas P. Resolution of high and low affinity epidermal growth factor receptors: inhibition of high affinity component by low temperature, cycloheximide, and phorbol esters. J. Biol. Chem. 1982; 257: 3053–3060
- Chatelier R. C., Ashcroft R. G., Lloyd C. J., Nice E. C., Whitehead R. H., Sawyer W. H., Burgess A. W. Binding of fluoresceinated epidermal growth factor to A431 cell sub‐populations studied using a model‐independent analysis of flow cytometric fluorescence data. EMBO J. 1986; 5: 1181–1186
- Gladhaug I. P., Christoffersen T. Kinetics of epidermal growth factor binding and processing in isolated intact rat hepatocytes: dynamic externalization of receptors during ligand internalization. Eur. J. Biochem. 1987; 164: 267–275
- Scatchard G. The attractions of proteins for small molecules and ions. Ann. NY Acad. Sci. 1949; 51: 660–672
- Kermode J. C., Becker E. L. Heterogeneity of binding of chemotactic formyl peptides to their receptors on rabbit neutrophils: methodological and analytical considerations in the study of complex receptor‐binding patterns. J. Recept. Res. 1986; 6: 247–270
- Kermode J. C. The curvilinear Scatchard plot: experimental artifact or receptor heterogeneity?. Biochem. Pharmacol. 1989; 38: 2053–2060
- Carpenter G., Lembach K. J., Morrison M. M., Cohen S. Characterization of the binding of 125I‐labeled epidermal growth factor to human fibroblasts. J. Biol. Chem. 1975; 250: 4297–4304
- Lin P. H., Selinfreund R., Wakshull E., Wharton W. Rapid and efficient purification of plasma membranes from cultured cells: characterization of epidermal growth factor binding. Biochemistry 1987; 26: 731–736
- Munson P. J. Experimental artifacts and the analysis of ligand binding data: results of a computer simulation. J. Recept. Res. 1983; 3: 249–259
- Taylor S. I. Binding of hormones to receptors: an alternative explanation of nonlinear Scatchard plots. Biochemistry 1975; 14: 2357–2361
- Kermode J. C., Thompson B. D. Iodination of thyroid‐stimulating hormone for receptor‐binding studies with human thyroid membranes: effects of specific activity and method of iodination. J. Endocrinol. 1980; 84: 439–447
- Kermode J. C. Means for the assessment of radioligand quality and its importance in receptor‐binding studies: observations with radiolabelled formylmethionyl‐leucyl‐phenylalanine. Biochem. J. 1988; 252: 521–528
- Greenwood F. C., Hunter W. M., Glover J. S. The preparation of 131I‐labelled human growth hormone of high specific radioactivity. Biochem. J. 1963; 89: 114–123
- Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I‐containing acylating agent: application to the radioimmunoassay. Biochem. J. 1973; 133: 529–539
- Kermode J. C., Thompson B. D., Edmonds C. J. Comparison of binding of bovine and human thyroid‐stimulating hormone to receptor sites on human thyroid membranes. J. Endocrinol. 1981; 88: 205–217
- Savage C. R., Hash J. H., Cohen S. Epidermal growth factor: location of disulfide bonds. J. Biol. Chem. 1973; 248: 7669–7672
- Gregory H. Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature 1975; 257: 325–327
- Thompson B. D., Kermode J. C., Unpublished observations
- Manley S. W., Bourke J. R., Hawker R. W. The thyrotrophin receptor in guinea‐pig thyroid homogenate: general properties. J. Endocrinol. 1974; 61: 419–436
- Birnbaumer L., Swartz T. L., Rojas F. J., Garber A. J. Synthesis, purification by HPLC and characterization of monoiodoglucagon labeled with carrier free 125I: use as glucagon receptor probe. J. Recept. Res. 1983; 3: 3–20
- Linde S., Hansen B., Lernmark Å. Preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis. Methods Enzymol. 1983; 92: 309–335
- McMaster D., Suzuki Y., Rorstad O., Lederis K. Iodinated derivatives of vasoactive intestinal peptide (VIP), PHI and PHM: purification, chemical characterization and biological activity. Peptides 1987; 8: 663–676
- Komoriya A., Hortsch M., Meyers C., Smith M., Kanety H., Schlessinger J. Biologically active synthetic fragments of epidermal growth factor: localization of a major receptor binding region. Proc. Natl. Acad. Sci. USA 1984; 81: 1351–1355