6
Views
10
CrossRef citations to date
0
Altmetric
Research Article

A System to Select for Mutant LLC-PK1 Cells Affected in camp Mediated Hormonal Response Using a Photoactivatable Analogue of Vasopressin

, &
Pages 61-80 | Published online: 26 Sep 2008

References

  • Gilman A. G., Sternweis P. C., Howlett A. C., Ross E. M. Biochemical and genetic resolution of components of the S49 lymphoma adenylase cyclase system. Hormones and cell culture, part A, G. H. Sato, R. Ross. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 1979; 299–315
  • Gilman A. G. G-proteins and dual control of adenylate cyclase. Cell 1984; 36: 577–579
  • Steinberg R. A. Molecular approaches to the study of cyclic AMP action 1983 in Biochemical actions of hormones, G. Litwack. Academic Press, New York, Vol. 11: 25–65
  • Lohmann S. M., Walter U. Regulation of the cellular and subcellular concentrations and distribution of cyclic nucleotide-dependent protein kinase. Adv.Cyclic Nucleotide Protein Phosphorylation Res. 1984; 18: 63–117
  • Hemmings B. A. Regulation of cAMP-dependent protein kinase in cultured cells. Curr. Topics Cell Regulation 1985; 27: 117–132
  • Hull R. N., Cherry W. R., Weaver G. W. The origin and characteristics of a pig kidney cell strain. LLC-PK1 In vitro 1976; 12: 670–677
  • Dayer J. M., Vassalli J. D., Bobbit J. L., Hull R. N., Reich E., Krane S. M. Calcitonin stimulates plasminogen activator in porcine renal tubular cells: LLC-PK1. J.Cell Biol. 1981; 91: 195–200
  • Jans D. A., Resink T. J., Wilson E. R., Reich E., Hemmings B. A. Isolation of a mutant LLC-PK1 cell line defective in hormonal responsiveness: a pleiotropic lesion in receptor function. Eur.J.Biochem. 1986; 160: 407–412
  • Jans D. A., Resink T. J., Hemmings B. A. Complementation between LLC-PK1 mutants affected in polypeptide hormone receptor function. Eur.J.Biochem. 1987; 162: 571–576
  • Nagamine Y., Sudol M., Reich E. Hormonal regulation of plasminogen activator mRNA production in porcine kidney cells. Cell 1983; 32: 1181–1190
  • Jans D. A., Hemmings B. A. LLC-PK1 cell mutants in cAMP metabolism respond normally to phorbol esters. FEBS Lett. 1986; 205: 127–131
  • Jans D. A., Hemmings B. A. cAMP metabolism in the porcine kidney epithelial cell line LLC-PK1: the central role of the cAMP dependent protein kinase in CAMP mediated gene induction. Adv. in Second Messenger and Phosphoprotein Res. 1988; 21: 109–121
  • Fahrenholz F., Boer R., Crause P., Toth M. V. Photoaffinity labelling of the renal V2 vasopressin receptor -identification and enrichment of a vasopressin-binding subunit. Eur.J.Biochem. 1985; 152: 589–595
  • Fahrenholz F., Eggena P., Gazis D., Toth M. V., Schwartz I. L. Photoreactive neurohypophyseal hormone analogs: effects on transport processes in mammalian and amphibian epithelia. Endocrinology 1986; 118: 1026–1031
  • Eggena P., Fahrenholz F., Schwartz I. A. Irreversible stimulation of hydroosmotic response in toad bladder by photoaffinity labelling with Phe2 Phe(p-N3)3 vasopressin. Endocrinology 1983; 113: 1413–1421
  • Eggena P., Fahrenholz F., Schwartz I. A. Covalent linkage of Phe2 Phe(p-N3)3 AVP to vasopressin receptors in toad bladder during photolysis. Am.J.Physiol. 1984; 246: C486–C493
  • Jans D. A., Gajdas E. L., Dierks-Ventling C., Hemmings B. A., Fahrenholz F. Long term stimulation of cAMP production in LLC-PK1 pig kidney cells by salmon calcitonin or a photoacti-vatable analogue of vasopressin. Biochim. Biophys.Acta 1987; 930: 392–400
  • Finlay G. J., Baguley B. C., Wilson W. R. A semi automated microculture method for investigating growth inhibitory effects of cytotoxic compounds on exponentially growing carcinoma cells. Anal.Biochem. 1984; 139: 272–277
  • Jans D. A., Resink T. J., Hemmings B. A. The dependence of urokinase-type plasminogen activator induction on cAMP-dependent protein kinase activation in LLC-PK1 cells. Biochem.J. 1987; 243: 413–418
  • Devis P. E., Grohol S. H., Taub M. Dibutyryl cyclic AMP resistant MDCK cells in serum-free medium have reduced cyclic AMP dependent protein kinase activity and a diminished effect of PGE1 on differentiated function. J.Cell. Physiol. 1985; 125: 23–25
  • Lamp S. J., Findlay D. M., Moseley J. M., Martin T. J. Calcitonin induction of a persistent activated state of adenylate cyclase in human breast cancer cells (T47D). J.Biol.Chem. 1981; 256: 12269–12274
  • Bradford W. M. A rapid, sensitive method for the question of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 72: 248–249
  • Fahrenholz F., Boer R., Crause P., Fritzsch G., Grzonka Z. Interactions of vasopressin agonists and antagonists with membrane receptors. Eur.J.Pharmacol. 1984; 100: 47–58
  • Steinberg R. A., Steinberg M. G., Van Daalen Wetters T. A variant of S49 mouse lymphoma cells with enhanced secretion of cyclic AMP. J.Cell.Physiol. 1979; 100: 579–588
  • Frimmer M., Ziegler K. Photoaffinity labeling of whole cells by flashed light: a simple apparatus for high energy ultraviolet flashes. Biochim.Biophys.Acta 1986; 855: 143–146
  • Fahrenholz F., Hackenberg M., Müller M. Identification of a myometrial oxytocin-receptor protein. Eur.J.Biochem. 1988; 174: 81–85
  • Sibley D. R., Lefkowitz R. J. Molecular mechanisms of receptor desensitization using the β-adrenergic receptor-coupled adenylate cyclase system as a model. Nature 1987; 317: 124–129
  • Benovic J. L., Bouvier M., Caron M. G., Lefkowitz R. J. Regulation of adenyl cyclase-coupled β-adrenergic receptors. Ann.Rev.Cell Biol. 1988; 4: 405–428

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.