Labelling of Rat Brain ß-Adrenoceptors: (³H)CGP-12177 or (¹²⁵I)Iodocyanopindolol?

References

• Nahorski S. R. Association of high affinity stereospecific binding of (3H)propranolol to cerebellar membranes with beta-adrenoceptors. Nature 1976; 259: 488–489
   PubMedGoogle Scholar
• Bylund D. B., Snyder S. H. Beta-adrenergic receptor binding in membrane preparations from mammalian brain. Mol. Pharmacol. 1976; 12: 568–580
   PubMed Web of Science ®Google Scholar
• Stone E. A., U'Prichard D. C. (3H)dihydroalprenolol binding in the rat brainstem. Eur. J. Pharmacol. 1981; 75: 159–161
   Google Scholar
• Riva M. A., Creese I. Comparison of two putative selective radioligands for labeling central nervous system ß-adrenergic receptors: Inadequacy of [3H]dihydroalprenolol. Mol. Pharmacol. 1989; 36: 201–210
   Google Scholar
• Aurbach G. D., Fedak S. A., Woodard C. J., Palmer J. S., Hauser D., Troxler T. The beta-adrenergic receptor: stereospecific interaction of iodinated beta-blocking agent with a high affinity state. Science 1974; 186: 1220–124
   Google Scholar
• Barovsky K., Brooker G. (-)[125I]-iodopindolol, a new highly selective radioiodinated ß-adrenergic receptor antagonist: Measurement of ß-receptors on intact rat astrocytoma cell. J. Cyclic Nucleotide Res. 1980; 6: 297–307
   PubMedGoogle Scholar
• Engel G., Hoyer D., Berthold R., Wagner H. (125Iodo)cyanopindolol, a new ligand for ß-adrenoceptors: identification and quantitation of subclasses of ß-adrenoceptors in guinea Pig. Naunyn-Schmiedeberg's Arch-Pharmacol. 1981; 317: 277–285
   Google Scholar
• Petrovic S. L., McDonald J. K., Snyder G. D., McCann S. M. Characterization of ß-adrenergic receptors in rat brain and pituitary using a new high affinity ligand, [125I]iodocyanopindolol. Brain. Res. 1983; 261: 249–259
   PubMed Web of Science ®Google Scholar
• Morin D., Zini R., Brunner F., Sebille B., Tillement J. P. Thermodynamic study of iodocyanopindolol ß-adrenoceptor interactions with rat lung and cerebral cortex. J. Receptor. Res. 1983–84; 3: 773–790
   PubMed Web of Science ®Google Scholar
• Willcoks A. L., Nahorski S. R. Binding of 125I-iodohydroxybenzylpindolol to cerebral membranes: association with 5-hydroxytryptamine recognition sites as well as beta-adrenoceptors. Biochem. Pharmacol. 1983; 32: 3311–3319
   Google Scholar
• Hoyer D., Engel G., Kalkman H. O. Characterization of the 5HT1B recognition site in rat brain: binding studies with (-)(125I)iodocyanopindolol. Eur. J. Pharmacol. 1985; 118: 1–12
   PubMedGoogle Scholar
• Neve K. A., McGonigle P., Molinoff P. B. Quantitative analysis of the selectivity of radioligands for subtypes of beta-adrenergic receptors. J. Pharmacol. Exp. Ther. 1986; 238: 46–53
   PubMedGoogle Scholar
• Staehelin M., Simons P., Jaeggi K., Wigger N. CGP-12177: a hydrophobic ß-adrenergic receptor radioligand reveals high affinity binding of agonists to intact cells. J. Biol. Chem. 1983; 258: 3496–3502
   PubMed Web of Science ®Google Scholar
• Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurements with the folin reagent. J. Biol. Chem. 1951; 193: 265–271
   PubMed Web of Science ®Google Scholar
• Weiland G. A., Molinoff P. B. Minireview: quantitative analysis of drug-receptor interactions: I determination of kinetic and equilibrium properties. Life Sci. 1981; 29: 313–330
   PubMed Web of Science ®Google Scholar
• Cheng Y. C., Prusoff W. H. Relationships between the inhibition constant (Ki) and the concentration of inhibitor which causes 50 per cent inhibition (IC50) of an enzymatic reaction. Biochem. Pharmacol. 1973; 22: 3099–3108
   PubMed Web of Science ®Google Scholar
• Scatchard G. The attractions of proteins for small molecules and ions. Ann. N. Y. Sci. 1949; 51: 660–672
   Web of Science ®Google Scholar
• Dooley D. J., Bittiger H., Reymann N. C. CGP 20712A: a useful tool for quantitating ß1- and ß2-adrenoceptors. Eur. J. Pharmacol. 1986; 130: 137–139
   Google Scholar
• Offord S. J., Ordway G. A., Frazer A. Application of (125I)iodocyanopindolol to measure 5-hydroxytrypt-amine1B receptors in the brain of the rat. J. Pharmacol. Exp. Ther. 1988; 244: 144–153
   PubMedGoogle Scholar
• O'Donnel J. M., Wolfe B. B., Frazer A. Agonist interactions with beta-adrenergic receptors in rat brain. J. Pharmacol. Exp. Ther. 1984; 228: 640–647
   Google Scholar
• Schutz W., Nanoff C., Freissmuth M. The fallacy of non-selectivity of radioactive ligands. TIPS 1988; 9: 261–264
   Google Scholar
• McGonigle P., Neve K. A., Molinoff P. B. A quantitative method of analysing the interaction of slightly selective radioligands with multiple receptor subtypes. Mol. Pharmacol. 1986; 30: 329–337
   Google Scholar
• Nanoff C., Freissmuth H., Schutz W. The role of a low ß1-adrenoceptor selectivity of (3H)CGP-12177 for resolving subtype-selectivity of competitive ligands. Naunyn-Schmiedeberg's Arch. Pharmacol. 1987; 336: 519–527
   Google Scholar
• Tsuchihashi H., Yokoyama H., Nagatomo T. Binding characteristics of (3H)-CGP-12177 to ß-adrenoceptors in rat myocardial membranes. Japan J. Pharmacol. 1989; 49: 11–19
   Google Scholar
• Minneman K. P., Hegstrand L. R., Molinoff P. B. Simultaneous determination of ß1- and ß2-adrenergic receptors in tissues containing both receptor subtypes. Mol. Pharmacol. 1979; 16: 34–46
   PubMed Web of Science ®Google Scholar
• Beer M., Richardson A., Poat J., Iversen L. L., Stahl S. M. In vitro selectivity of agonists and antagonists for betal-and beta2-adrenoceptor subtypes in rat brain. Biochem. Pharmacol. 1988; 37: 1145–1151
   Google Scholar
• Rainbow T. C., Parsons B., Wolfe B. B. Quantitative autoradiography of ß1- and ß2-adrenergic receptors in rat brain. Proc. Natl. Acad. Sci. 1984; 81: 1585–1589
   PubMedGoogle Scholar
• Dooley D. J., Heal D. J., Goodwin G. M. Repeated electroconvulsive shock prevents increased neocortical ß1-adrenoceptor binding after DSP-4 treatment in rats. Eur. J. Pharmacol. 1986; 134: 333–337
   Google Scholar
• Heal D. J., Butler S. A., Hurst E. M., Buckett W. R. Antidepressant treatments, including sibutramine hydrochloride and electroconvulsive shock, decrease ß1- but not ß2-adrenoceptors in rat cortex. J. Neurochem. 1989; 53: 1019–1025
   PubMedGoogle Scholar