The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model

References

• Abd-El-Aleem SA, Ferguson MWJ, Appleton I, Bhowmick A, Mccollum CN. Ireland GW. (2001). Expression of cyclooxygenase isoforms in normal human skin and chronic venous ulcers. J Pathol, 195, 616–623.
   PubMed Web of Science ®Google Scholar
• Hashem MA, Jun KY, Lee E, Lim S, Choo HY, Kwon Y. (2008). A rapid and sensitive screening system for human type I collagen with the aim of discovering potent anti-aging or anti-fibrotic compounds. Mol Cells, 26, 625–630.
   PubMed Web of Science ®Google Scholar
• Annan K, Houghton PJ. (2008). Antibacterial, antioxidant and fibroblast growth stimulation of aqueous extracts of Ficus asperifolia Miq. and Gossypium arboreum L., wound-healing plants of Ghana. J Ethnopharmacol, 119, 141–144.
   PubMed Web of Science ®Google Scholar
• Asghar Z, Masood Z. (2008). Evaluation of antioxidant properties of silymarin and its potential to inhibit peroxyl radicals in vitro. Pak J Pharm Sci, 21, 249–254.
   PubMed Web of Science ®Google Scholar
• Bradford MM. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 72, 248–254.
   PubMed Web of Science ®Google Scholar
• Diegelmann RF, Evans MC. (2004). Wound healing: An overview of acute, fibrotic and delayed healing. Front Biosci, 9, 283–289.
   PubMed Web of Science ®Google Scholar
• Dovi JV, He LK, DiPietro LA. (2003). Accelerated wound closure in neutrophil-depleted mice. J Leukoc Biol, 73, 448–455.
   PubMed Web of Science ®Google Scholar
• Futagami A, Ishizaki M, Fukuda Y, Kawana S, Yamanaka N. (2002). Wound healing involves induction of cyclooxygenase-2 expression in rat skin. Lab Invest, 82, 1503–1513.
   PubMed Web of Science ®Google Scholar
• Gu M, Singh RP, Dhanalakshmi S, Agarwal C, Agarwal R. (2007). Silibinin inhibits inflammatory and angiogenic attributes in photocarcinogenesis in SKH-1 hairless mice. Cancer Res, 67, 3483–3491.
   PubMed Web of Science ®Google Scholar
• Gupta A, Singh RL, Raghubir R. (2002). Antioxidant status during cutaneous wound healing in immunocompromised rats. Mol Cell Biochem, 241, 1–7.
   PubMed Web of Science ®Google Scholar
• Gutiérrez-Fernández A, Inada M, Balbín M, Fueyo A, Pitiot AS, Astudillo A, Hirose K, Hirata M, Shapiro SD, Noël A, Werb Z, Krane SM, López-Otín C, Puente XS. (2007). Increased inflammation delays wound healing in mice deficient in collagenase-2 (MMP-8). FASEB J, 21, 2580–2591.
   PubMed Web of Science ®Google Scholar
• Houghton PJ, Hylands PJ, Mensah AY, Hensel A, Deters AM. (2005). In vitro tests and ethnopharmacological investigations: Wound healing as an example. J Ethnopharmacol, 100, 100–107.
   PubMed Web of Science ®Google Scholar
• Katiyar SK. (2002). Treatment of silymarin, a plant flavonoid, prevents ultraviolet light-induced immune suppression and oxidative stress in mouse skin. Int J Oncol, 21, 1213–1222.
   PubMed Web of Science ®Google Scholar
• Katiyar SK, Korman NJ, Mukhtar H, Agarwal R. (1997). Protective effects of silymarin against photocarcinogenesis in a mouse skin model. J Natl Cancer Inst, 89, 556–566.
   PubMed Web of Science ®Google Scholar
• Köksal E, Gülçin I, Beyza S, Sarikaya O, Bursal E. (2009). In vitro antioxidant activity of silymarin. J Enzyme Inhib Med Chem, 24, 395–405.
   PubMed Web of Science ®Google Scholar
• Lahiri-Chatterjee M, Katiyar SK, Mohan RR, Agarwal R. (1999). A flavonoid antioxidant, silymarin, affords exceptionally high protection against tumor promotion in the SENCAR mouse skin tumorigenesis model. Cancer Res, 59, 622–632.
   PubMed Web of Science ®Google Scholar
• Lau TW, Chan YW, Lau CP, Chan CM, Lau CB, Fung KP, Leung PC, Ho YY. (2007). Investigation of the effects of Chinese medicine on fibroblast viability: implications in wound healing. Phytother Res, 21, 938–947.
   PubMed Web of Science ®Google Scholar
• Meeran SM, Katiyar S, Elmets CA, Katiyar SK. (2006). Silymarin inhibits UV radiation-induced immunosuppression through augmentation of interleukin-12 in mice. Mol Cancer Ther, 5, 1660–1668.
   PubMed Web of Science ®Google Scholar
• Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci, 84, 407–412.
   PubMed Web of Science ®Google Scholar
• Nencini C, Giorgi G, Micheli L. (2007). Protective effect of silymarin on oxidative stress in rat brain. Phytomedicine, 14, 129–135.
   PubMed Web of Science ®Google Scholar
• Peng L, Wang B, Ren P. (2005). Reduction of MTT by flavonoids in the absence of cells. Colloids Surf B Biointerfaces, 45, 108–111.
   PubMed Web of Science ®Google Scholar
• Psotová J, Zahálková J, Hrbác J, Simánek V, Bartek J. (2001). Determination of total antioxidant capacity in plasma by cyclic voltammetry. Two case reports. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 145, 81–83.
   PubMedGoogle Scholar
• Ramakrishnan G, Raghavendran HR, Vinodhkumar R, Devaki T. (2006). Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem Biol Interact, 161, 104–114.
   PubMed Web of Science ®Google Scholar
• Raso GM, Meli R, Di Carlo G, Pacilio M, Di Carlo R. (2001). Inhibition of inducible nitric oxide synthase and cyclooxygenase-2 expression by flavonoids in macrophage J774A.1. Life Sci, 68, 921–931.
   PubMed Web of Science ®Google Scholar
• Russo A, Longo R, Vanella A. (2002). Antioxidant activity of propolis: Role of caffeic acid phenethyl ester and galangin. Fitoterapia, 73 Suppl 1, S21–S29.
   Web of Science ®Google Scholar
• Schümann J, Prockl J, Kiemer AK, Vollmar AM, Bang R, Tiegs G. (2003). Silibinin protects mice from T cell-dependent liver injury. J Hepatol, 39, 333–340.
   PubMed Web of Science ®Google Scholar
• Singer AJ, Clark RA. (1999). Cutaneous wound healing. N Engl J Med, 341, 738–746.
   PubMed Web of Science ®Google Scholar
• Soneja A, Drews M, Malinski T. (2005). Role of nitric oxide, nitroxidative and oxidative stress in wound healing. Pharmacol Rep, 57 Suppl, 108–119.
   PubMed Web of Science ®Google Scholar
• Stallmeyer B, Kämpfer H, Kolb N, Pfeilschifter J, Frank S. (1999). The function of nitric oxide in wound repair: inhibition of inducible nitric oxide-synthase severely impairs wound reepithelialization. J Invest Dermatol, 113, 1090–1098.
   PubMed Web of Science ®Google Scholar
• Stipcevic T, Piljac J, Vanden Berghe D. (2006). Effect of different flavonoids on collagen synthesis in human fibroblasts. Plant Foods Hum Nutr, 61, 29–34.
   PubMed Web of Science ®Google Scholar
• Svobodová A, Walterová D, Psotová J. (2006). Influence of silymarin and its flavonolignans on H(2)O(2)-induced oxidative stress in human keratinocytes and mouse fibroblasts. Burns, 32, 973–979.
   PubMed Web of Science ®Google Scholar
• Svobodová A, Zdarilová A, Malisková J, Mikulková H, Walterová D, Vostalová J. (2007). Attenuation of UVA-induced damage to human keratinocytes by silymarin. J Dermatol Sci, 46, 21–30.
   PubMed Web of Science ®Google Scholar
• Toklu HZ, Tunali-Akbay T, Erkanli G, Yüksel M, Ercan F, Sener G. (2007). Silymarin, the antioxidant component of Silybum marianum, protects against burn-induced oxidative skin injury. Burns, 33, 908–916.
   PubMed Web of Science ®Google Scholar
• Turgut F, Bayrak O, Catal F, Bayrak R, Atmaca AF, Koc A, Akbas A, Akcay A, Unal D. (2008). Antioxidant and protective effects of silymarin on ischemia and reperfusion injury in the kidney tissues of rats. Int Urol Nephrol, 40, 453–460.
   PubMed Web of Science ®Google Scholar
• Wilgus TA, Bergdall VK, Tober KL, Hill KJ, Mitra S, Flavahan NA, Oberyszyn TM. (2004). The impact of cyclooxygenase-2 mediated inflammation on scarless fetal wound healing. Am J Pathol, 165, 753–761.
   PubMed Web of Science ®Google Scholar
• Wilgus TA, Vodovotz Y, Vittadini E, Clubbs EA, Oberyszyn TM. (2003). Reduction of scar formation in full-thickness wounds with topical celecoxib treatment. Wound Repair Regen, 11, 25–34.
   PubMed Web of Science ®Google Scholar
• Zhao J, Lahiri-Chatterjee M, Sharma Y, Agarwal R. (2000). Inhibitory effect of a flavonoid antioxidant silymarin on benzoyl peroxide-induced tumor promotion, oxidative stress and inflammatory responses in SENCAR mouse skin. Carcinogenesis, 21, 811–816.
   PubMed Web of Science ®Google Scholar
• Zhao J, Sharma Y, Agarwal R. (1999). Significant inhibition by the flavonoid antioxidant silymarin against 12-O-tetradecanoylphorbol 13-acetate-caused modulation of antioxidant and inflammatory enzymes, and cyclooxygenase 2 and interleukin-1α expression in SENCAR mouse epidermis: Implications in the prevention of stage I tumor promotion. Mol Carcinog, 26, 321–333.
   PubMed Web of Science ®Google Scholar
• Zi X, Mukhtar H, Agarwal R. (1997). Novel cancer chemopreventive effects of a flavonoid antioxidant silymarin: inhibition of mRNA expression of an endogenous tumor promoter TNF alpha. Biochem Biophys Res Commun, 239, 334–339.
   PubMed Web of Science ®Google Scholar