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Research Article

Single-platform quality control assay to quantify multipotential stromal cells in bone marrow aspirates prior to bulk manufacture or direct therapeutic use

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Pages 431-440 | Received 27 Oct 2011, Accepted 14 Dec 2011, Published online: 24 Jan 2012

References

  • Tolar J, Villeneuve P, Keating A. Mesenchymal stromal cells for graft-versus-host disease. Hum Gene Ther. 2011;22: 257–62.
  • Dryden GW. Overview of stem cell therapy for Crohn's disease. Expert Opin Biol Ther. 2009;9:841–7.
  • Jones KB, Seshadri T, Krantz R, Keating A, Ferguson PC. Cell-based therapies for osteonecrosis of the femoral head. Biol Blood Marrow Transplant. 2008;14:1081–7.
  • Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, . Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7.
  • Tarte K, Gaillard J, Lataillade JJ, Fouillard L, Becker M, Mossafa H, . Clinical-grade production of human mesenchymal stromal cells: occurrence of aneuploidy without transformation. Blood. 2010;115:1549–53.
  • Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R, . Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS ONE. 2008;3:e2213.
  • Stolzing A, Jones E, McGonagle D, Scutt A. Age-related changes in human bone marrow-derived mesenchymal stem cells: consequences for cell therapies. Mech Ageing Develop. 2008;129:163–73.
  • Baxter MA, Wynn RF, Jowitt SN, Wraith JE, Fairbairn LJ, Bellantuono I. Study of telomere length reveals rapid aging of human marrow stromal cells following in vitro expansion. Stem Cells. 2004;22:675–82.
  • Wagner W, Bork S, Lepperdinger G, Joussen S, Ma N, Strunk D, . How to track cellular aging of mesenchymal stromal cells? Aging. 2010;2:224–30.
  • Halfon S, Abramov N, Grinblat B, Ginis I. Markers distinguishing mesenchymal stem cells from fibroblasts are downregulated with passaging. Stem Cells Develop. 2011;20:53–66.
  • Jones E, English A, Churchman SM, Kouroupis D, Boxall SA, Kinsey S, . Large-scale extraction and characterization of CD271+ multipotential stromal cells from trabecular bone in health and osteoarthritis: implications for bone regeneration strategies based on uncultured or minimally cultured multipotential stromal cells. Arthritis Rheum. 2010;62:1944–54.
  • Tormin A, Brune JC, Olsson E, Valcich J, Neuman U, Olofsson T, . Characterization of bone marrow-derived mesenchymal stromal cells (MSC) based on gene expression profiling of functionally defined MSC subsets. Cytotherapy. 2009;11:114–28.
  • Wagner W, Bork S, Horn P, Krunic D, Walenda T, Diehlmann A, . Aging and replicative senescence have related effects on human stem and progenitor cells. PLoS ONE. 2009;4:e5846.
  • D'Ippolito G, Schiller PC, Ricordi C, Roos BA, Howard GA. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res. 1999;14:1115–22.
  • Hernigou P, Poignard A, Beaujean F, Rouard H. Percutaneous autologous bone-marrow grafting for nonunions: influence of the number and concentration of progenitor cells. J Bone Joint Surgery. 2005;87A:1430–7.
  • Veyrat-Masson R, Boiret-Dupre N, Rapatel C, Descamps S, Guillouard L, Guerin J-J, . Mesenchymal content of fresh bone marrow: a proposed quality control method for cell therapy. Br J Haematol. 2007;139:312–20.
  • Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Maeyers P, Chiarieri D, . Characterisation of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood. 1980;56:289–301.
  • Dobson K, Reading L, Scutt A. A cost-effective method for the automatic quantitative analysis of fibroblastic colony-forming units. Calcified Tissue Int. 1999;65:166–72.
  • Kasten P, Beyen I, Egermann M, Suda AJ, Moghaddam AA, Zimmermann G, . Instant stem cell therapy: characterization and concentration of human mesenchymal stem cells in vitro. Eur Cells Mat. 2008;16:47–55.
  • Quirici N, Soligo D, Bossolasco P, Servida F, Lumini C, Deliliers GL. Isolation of bone marrow mesenchymal stem cells by anti-nerve growth factor receptor antibodies. Exp Hematol. 2002;30:783–91.
  • Jones EA, English A, Kinsey SE, Emery P, McGonagle DG, Jones RA, . Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arth Rheum. 2002;46:3349–60.
  • Buhring H-J, Battula VL, Treml S, Schewe B, Kanz L, Vogel W. Novel markers for the prospective isolation of human MSC. Ann NY Acad Sci. 2007;1106:262–71.
  • Jones EA, English A, Kinsey SE, Straszynski L, Emery P, Ponchel F, . Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytom B: Clin Cytom. 2006;70:391–9.
  • Tormin A, Li O, Brune JC, Walsh S, Schütz B, Ehinger M, . CD146 expression on primary non-hematopoietic bone marrow stem cells correlates to in situ localization. Blood. 2011;117:5061–77.
  • Galotto M, Berisso G, Delfino L, Podesta M, Ottaggio L, Dallorso S, . Stromal damage as consequence of high-dose chemo/radiotherapy in bone marrow transplant recipients. Exp Hematol. 1999;27:1460–6.
  • Cox G, McGonagle D, Boxall SA, Buckley CT, Jones E, Giannoudis PV. The use of the reamer-irrigator-aspirator to harvest mesenchymal stem cells. J Bone Joint Surg Br Vol. 2011;93B:517–24.
  • Seebach C, Henrich D, Tewksbury R, Wilhelm K, Marzi I. Number and proliferative capacity of human mesenchymal stem cells are modulated positively in multiple trauma patients and negatively in atrophic nonunions. Calc Tissue Int. 2007;80:294–300.
  • Keeney M, Chin-Yee I, Weir K, Popma J, Nayar R, Sutherland DR. Single platform flow cytometric absolute CD34+ cell counts based on the ISHAGE guidelines. International Society of Hematotherapy and Graft Engineering. Cytometry. 1998;34:61–70.
  • Jones EA, Kinsey SE, English A, Jones RA, Straszynski L, Meredith DM, . Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arth Rheum. 2002;46:3349–60.
  • Piedras-Ross J, Leon-Rodriguez E, Sanchez-Guerrero S, Lopez-Karpovitch X. Comparison of single- and dual-platform approaches to enumerate CD34(+) cells in bone marrow and mobilized peripheral blood stem cells. Arch Med Res. 2003;34:16–9.
  • Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH. Isolation and characterization of size-sieved stem cells from human bone marrow. Stem Cells. 2002;20:249–58.
  • Lemarie C, Bouchet G, Sielleur I, Durand V, Navarro F, Calmels B, . A new single-platform method for the enumeration of CD34+ cells. Cytotherapy. 2009;11:804–6.
  • Sutherland DR, Nayyar R, Acton E, Giftakis A, Dean S, Mosiman VL. Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers. Cytotherapy. 2009;11: 595–605.
  • Fritsch G, Buchinger P, Printz D. Use of flow cytometric CD34 analysis to quantify hematopoietic progenitor cells. Leuk Lymphoma. 1993;10:443–51.
  • Hernigou P, Poignard A, Manicom O, Mathieu G, Rouard H. The use of percutaneous autologous bone marrow transplantation in nonunion and avascular necrosis of bone. J Bone Joint Surg Br Vol. 2005;87B:896–902.
  • Jones E, McGonagle D. Human bone marrow mesenchymal stem cells in vivo. Rheumatology. 2008;47:126–31.

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