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Short Report

Usefulness of a 16S rDNA real-time PCR to monitor neonatal sepsis and to assist in medical decision to discontinue antibiotics

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Pages 2141-2144 | Received 03 Jun 2015, Accepted 24 Jul 2015, Published online: 28 Aug 2015

References

  • Liu L, Johnson HL, Cousens S, et al. Global, regional, and national causes of child mortality: an updated systematic analysis for 2010 with time trends since 2000. Lancet 2012;379:2151–61
  • Pammi M, Flores A, Leeflang M, Versalovic J. Molecular assays in the diagnosis of neonatal sepsis: a systematic review and meta-analysis. Pediatrics 2011;128:e973–85
  • Ohlin A, Bäckman A, Ewald U, et al. Diagnosis of neonatal sepsis by broad-range 16S real-time polymerase chain reaction. Neonatology 2012;101:241–6
  • Gosiewski T, Flis A, Sroka A, et al. Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis. BMC Microbiol 2014;14:313
  • Rogers GB, Stressmann FA, Koller G, et al. Assessing the diagnostic importance of nonviable bacterial cells in respiratory infections. Diagn Microbiol Infect Dis 2008;62:133–41
  • Jiang LJ, Wu WJ, Wu H, et al. Rapid detection and monitoring therapeutic efficacy of Mycobacterium tuberculosis complex using a novel real-time assay. J Microbiol Biotechnol 2012;22:1301–6
  • Goldstein B, Giroir B, Randolph A. International pediatric sepsis consensus conference: definitions for sepsis and organ dysfunction in pediatrics. Pediatr Crit Care Med 2005;6:2–8
  • Manroe BL, Weinberg AG, Rosenfeld CR, Browne R. The neonatal blood count in health and disease. J Pediatr 1979;95:89–98
  • Leuthner SR, Das UG. Low Apgar scores and the definition of birth asphyxia. Pediatr Clin North Am 2004;51:737–45
  • Pereira AP, Dias MA, Bastos MH, et al. Determining gestational age for public health care users in Brazil: comparison of methods and algorithm creation. BMC Res Notes 2013;6:60
  • Brozanski BS, Jones, JG, Krohn MJ, et al. Use of polymerase chain reaction as a diagnostic tool for neonatal sepsis can result in a decrease in use of antibiotics and total neonatal intensive care unit length of stay. J Perinatol 2006;26:688–92
  • Nocker A, Camper AK. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl Environ Microbiol 2006;72:1997–2004
  • Vendrame M, Iacumin L, Manzano M, Comi G. Use of propidium monoazide for the enumeration of viable Oenococcus oeni in must and wine by quantitative PCR. Food Microbiol 2013;35:49–57
  • Barbau-Piednoir E, Mahillon J, Pillyser J, et al. Evaluation of viability-qPCR detection system on viable and dead Salmonella serovar enteritidis. J Microbiol Methods 2014;103:131–7