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Paper from the 20th Summerschool in Immunotoxicology, Beaune, France, Sept. 26–28, 2011

Methods: Implementation of in vitro and ex vivo phagocytosis and respiratory burst function assessments in safety testing

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Pages 106-117 | Received 29 Jun 2012, Accepted 29 Sep 2012, Published online: 23 Nov 2012

References

  • Burleson, G. R., Dean, J. H., Munson, A. E. (Eds.) 1995. Methods in Immunotoxicology. Chapter 2. In: Isolation of Alveolar Macrophages, Peritoneal Macrophages, and Kupffer Cells. Vol. 2. (Lewis, J. G., Ed.). New York, NY: Wiley-Liss, Inc., pp. 15–26.
  • Butcher, S. K., Chahal, H., Nayak, L., Sinclair, A., Henriquez, N. V., Sapey, E., O’Mahony, D., Lord, J. M. 2001. Senescence in innate immune responses: Reduced neutrophil phagocytic capacity and CD16 expression in elderly humans. J. Leukocyte Biol. 70:881–886.
  • Butcher, S. K., Killampalli, V., Chahal, H., Kaya Alpar, E., Lord, J. M. 2003. Effect of age on susceptibility to post-traumatic infection in the elderly. Biochem. Soc. Trans. 31:449–451.
  • Cathcart, R., Schwiers, E., Ames, B. N. 1983. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. Anal. Biochem. 134:111–116.
  • Chan, H., Kedzierska, K., O’Mullane, J., Crowe, S. J., Jaworowski, A. 2001. Quantifying complement-mediated phagocytosis by human monocyte-derived macrophages. Immunol. Cell Biol. 79:429–435.
  • Coxon, P. Y, Rane, M. J., Uriarte, S., Powell, D. W., Singh, S., Butt, W., Chen, Q., McLeish, K. R. 2003. MAPK-activated protein kinase-2 participates in p38 MAPK-dependent and ERK-dependent functions in human neutrophils. Cell Signal. 15:993–1001.
  • Dzik, J. M., Zielinski, Z., Cielsla, J., and Walajtys-Rode, E. 2010. Trichinella spiralis infection enhances protein kinase C phosphorylation in guinea pig alveolar macrophages. Parasite Immunol. 32:209–220.
  • Elliot, J. A., Winn, W. C. 1986. Treatment of alveolar macrophages with cytochalasin D inhibits uptake and subsequent growth of Legionella pneumophila. Infect. Immun. 51:31–36.
  • Foukas, L. C., Katsoulas, H. L., Paraskevopoulou, N., Metheniti, A., Lambropoulou, M., Marmaras, V. 1998. Phagocytosis of Escherichia coli by insect hemocytes requires both activation of the Ras/mitogen-activated protein kinase signal transduction pathway for attachment and β3 integrin for internalization. J. Biol. Chem. 273:14813–14818.
  • Hübell, K., Hegener, K., Schnell, R., Mansmann, G., Oberhäuser, F., Staib, P., Diehl, V., Engert, A. 1999. Suppressed neutrophil function as a risk factor for severe infection after cytotoxic chemotherapy in patients with acute non-lymphocytic leukemia. J. Ann. Hematol. 78:73–77.
  • Jackson, J. K., Lauener, R., Duronio, V., Burt, H. M. 1997. The involvement of phosphatidylinositol 3-kinase in crystal induced human neutrophil activation. J. Rheumatol. 24:341–348.
  • Klaus, G. G. 1973. Cytochalasin B. Dissociation of pinocytosis and phagocytosis by peritoneal macrophages. Exp. Cell Res. 79:73–78.
  • Lehmann, D. M., Seneviratne, A. M. P. B., Smrcka, A. V. 2007. Small molecule disruption of G-protein subunit signaling inhibits neutrophil chemotaxis and inflammation. Mol. Pharmacol. 73:410–418.
  • Price, K. D. 2010. Bacterial infections in cynomolgus moneys given small molecule immunomodulatory antagonists. J. Immunotoxicol. 7:128–137.
  • Rosales, C., Brown, E. J. 1991. Two mechanisms for IgG Fc-receptor-mediated phagocytosis by human neutrophils. J. Immunol. 146:3937–3944.
  • Rosenkranz, A. R., Schmaldienst, S., Stuhlmeier, K. M., Chen, W., Knapp, W., Zlabinger, G. J. 1992. A microplate assay for the detection of oxidative products using 2′,7′-dichlorofluorescin diacetate. J. Immunol. Meth. 156:39–45.
  • Schmaldienst, S., Horl, W. H. 1996. Bacterial infections during immunosuppression-immunosuppressive agents interfere not only with immune response, but also with polymorphonuclear cell function. Nephrol. Dial. Transplant. 11:1243.
  • Schnyder, B., Meunier, P. C, Car, B. D. 1998. Inhibition of kinases impairs neutrophil activation and killing of Staphylococcus aureus. Biochem. J. 331:489–495.
  • Schopfer, K., Douglas, S. D. 1976. Neutrophil function in children with kwashiorkor. J. Lab. Clin. Med. 88:450–461.
  • Takeshige, K., Minakami, S. 1981. Involvement of calmodulin in phagocytotic respiratory burst of leukocytes. Biochem. Biophys. Res. Com. 99:484–490.
  • Torres, M., Hall, F. L., O’Neill, K. 1993. Stimulation of human neutrophils with formyl-methionyl-leucyl-phenylalanine induces tyrosine phosphorylation and activation of two distinct mitogen-activated protein-kinases. J. Immunol. 150:1563–1577.
  • van der Vliet, A. 2008. NADPH oxidases in lung biology and pathology, host defense enzymes and more. Free Radic. Biol. Med. 44:938–955.
  • Voloshina, E. V., Prasol, E. A., Grachev, S. V., Prokhorenko, I. R. 2009. Effect of cytochalasin D on the respiratory burst of primed neutrophils activated with a secondary stimulus. Dokl Biochem. Biophys. 424:13–15.
  • Wan, C. P., Park, C. S., Lau, B. H. 1993. A rapid and simple microfluorometric phagocytosis assay. J. Immunol. Meth. 162:1–7.
  • Wang, R., Town, T., Gokarn, V., Flavell, R. A., Chandawarkar, R. Y. 2006a. HSP70 enhances macrophage phagocytosis by interaction with lipid raft-associated TLR-7 and up-regulating p38 MAPK and PI3K pathways. J. Surg. Res. 136:58–69.
  • Wang, X. M., Kim, H. P., Song, R., Choi, A. M. K. 2006b. Caveolin-1 confers anti-inflammatory effects in murine macrophages via the MKK3/p38 MAPK pathway. Am. J. Resp. Cell Mol. Biol. 34:434–442.
  • Ward, R. A., Nakamura, M., McLeish, K. R. 2000. Priming of the neutrophil respiratory burst involves p38 mitogen-activated protein kinase-dependent exocytosis of flavocytochrome b558-containing granules. J. Biol. Chem. 275:36713–36719.
  • Weinstein, R. J., Young, L. S. 1976. Neutrophil function in gram-negative rod bacteremia: The interaction between phagocytic cells, infecting organisms, and humoral factors. J. Clin. Invest. 58:8190–8199.
  • Yona, S., Lin, H., Dri, P., Davies, J. Q., Hayhoe, R. P. G., Lewis, S. M., Heinsbroek, S. E., Brown, K. A., Perretti, M., Hamann, J., Treacher, D. F., Gordon, S., Stacey, M. 2008. Ligation of the adhesion-GPCR EMR2 regulates human neutrophil function. FASEB J. 22:741–751.

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