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Research Article

The Toxins Purified from Tityus Serrulatus (Lutz & Mello)Venom

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Pages 457-481 | Published online: 28 Sep 2008

References

  • Miranda F., Kopeyan C., Rochat C., Rochat H., Lissitzky S. Purification of animal neurotoxins. Isolation and charac terization of eleven neurotoxins from the venom of the scorpion Androctonus australis Hector, Buthus occitanus tunetanus and Leiurus quinquestriatus quinquestriatus. Eur. J. Biochem. 1970; 16: 514
  • Catterall W. A. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes. Ann. Rev. Pharmacol. Toxicol. 1980; 20: 15
  • Catterall W. A. Molecular properties of voltage-sensitive sodium channels. Ann. Rev. Biochem. 1986; 55: 953
  • Catterall W. A., Trainer V., Baden D. G. Molecular properties of the sodium channels: a receptor formultiple neurotoxins. Bull. Soc. Pathol. Exot. 1992; 85(5 pt2)481
  • Barchi R. L. Probing the molecular structure of the voltage-dependent sodium channel. Annu. Rev. Neurosci. 1988; 11: 455
  • Strichartz G., Rando T., Wang G. K. An integrated view of the molecular toxinology of sodium channel gating in excitable cells. Ann. Rev. Neurosci. 1987; 10: 237
  • Beneski D. A., Catterall W. A. Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin. Proc. Natl. Acad. Sci. USA 1980; 77: 639
  • Zlotkin E., Miranda F., Rochat H. C. Chemistry and pharmacology of Buthinae scorpion venom. Arthropod venoms, S. Bettini. Springer Veralg, Berlin 1978; 317–369, (Handbook Experimental Pharmacology, 48), Cap. 14
  • Jover E., Couraud F., Rochat H. Two types of scorpion neurotoxins characterized by their binding to two separate receptor sites on rat brain synaptosomes. Biochem. Biophys. Res. Commun. 1980; 95(4)1607
  • Couraud F., Jover E., Dubois J. M., Rochat H. Two types of scorpion toxin receptor sites, one related to the activation, the other to the inactivation of the action potential sodium channel. Toxicon 1982; 40: 9
  • Warnick J. E., Albuquerque E. X., Diniz C. R. Electrophysiological observations on the action of the purified scorpion venom Tityustoxin on nerve and skeletal muscle of the rat. J. Pharmacol. Exp. Ther. 1976; 198(1)155
  • Martin-Eauclaire M. F. Etude des toxines de scorpions : purification et caractérisation chimique et biologique. Thèse d'Etat ès Sciences 1987
  • Martin-Eauclaire M. F., Céard B., Ribeiro A. M., Diniz C. R., Rochat H., Bougis P. E. Biochemical, pharmacological and genomic characterization of Ts IV, an α-toxin from the venom of the South Americ an scorpion Tityus serrulatus. FEBS lett. 1994; 342: 181
  • Kirsch G. E., Skattebl A., Possani C. D., Brown A. M. Modification of Na channel gating by an α-scorpion toxin from Tityus serrulatus. J. Gen. Physiol. 1989; 93: 67
  • Arantes E. C., Ricciopo-Neto F., Sampaio S. V., Vieira C. A., Giglio J. R. Isolation and characterization of TsTx-V, a new neurotoxin from Tityus serrulatus scorpion venom which delays the inactivation of sodium channels. Biochim. Biophys. Acta 1994; 1199(1)69
  • Cahalan M. D. Modification of sodium channel gating in frog myelinated nerve fibres by Centruroides sculpturatus scorpion venom. J. Physiol. 1975; 244: 511
  • Jover E., Martin-Moutot N., Couraud F., Rochat H. Binding of scorpion toxins to rat brain synaptosomal fractions. Effects of membrane potential ions and other neurotoxins. Biochemistry 1980; 19: 463
  • Diniz C. R. Chemical and pharmacological aspects of Tityniae venoms. Arthropod venoms, S. Bettini. Springer-Verlag, Berlin Heidelberg and New-York 1978; 48: 379
  • Vital Brazil M. C. Contribuiçao ao estudo do envenenamento pela picada do escorpiao e seu tratamento. Coletanea de trabalhos do Instituto Butantan. Sao Paulo. Inst. Butantan 1909; 69: 81
  • Maurano H. R. Do Escorpionismo. (Thesis), 1915, Facultade de Medicina do Rio de Janeiro, Rio de Janeiro. Rodriguez & Cia (Jornal de Comércio)
  • Houssay B. A. Action Physiologique du venin des scorpions Buthus quinquestriatus et Tityus bahiensis. J. Physiol. P ath. Gén. 1919; 18: 305
  • Catterall W. A. Cooperative activation of action potential sodium ionophore by neurotoxins. Proc. Natl. Sci. USA 1975; 72(5)1782
  • Diniz C. R., Goncalves J. M. Some chemical and pharmacological properties of Brazilian scorpion venoms. Venoms, E. Buckley, N. Porges. A.A.A.S. Press, Washington 1956; 44: 131–9
  • Diniz C. R., Goncalves J. M. Separation of biologically active components from scorpion venoms by zone electrophoresis. Biochim. Niophys., Acta 1960; 41: 470
  • Diniz C. R., Valeri V. Effects of a toxin present in a purified extract of telson from the scorpion Tityus serrulatus on smooth muscle preparations and in mice. Arch. Int. Pharmacodyn. 1959; 71: 1
  • Diniz C. R., Torres G. M. Release of an achetylcholine-like substance from guinea pig ileum by scorpion venom. Toxicon 1968; 5: 227
  • Gomez M. V., Diniz C. R. Separation of toxic components from the Brazilian scorpion Tityus serrulatus venom. Mem. Inst. Butantan b 1966; 33: 899
  • Gomez M. V. Purificacao e caracterizaçao do toxina do escorpiao Tityus serrulatus. Departamento de Bioquimica. Universidade Federal de Minas Gerais, Belo Horizonte, MG 1967; 68, Thesis
  • Coutinho-Netto J., Diniz C. R. Properties of Tityustoxin obtained by a new method of purification. Ann. Acad. Bras. Cienc. 1974; 46(3–4)698, (Abstract)
  • Gomez M. V., Dai M. E.M., Diniz C. R. Effect of scorpion venom, Tityustoxin, on the release of acetylcholine from incubated slices of rat br ain. J. Neurochem. 1973; 20: 1051
  • Diniz C. R., Pimenta A. F., Coutinho-Netto J., Pompolo Pompolo J. S., Gomez M. V., Bohm G. M. Effect of scorpion venom from Tityus serrulatus (Tityustoxin) on the acetylcholine release and fine structure of nerve terminal. Experentia 1974; 30: 1304
  • Henriques M. C. Liberaçao de acetilcholina por Tityustoxina em systema nervoso periférico. Departamento de Bioquimica. Thesis, Univesidade Federal de Minas Gerais, Belo Horizonte, MG 1973; 108
  • Gomez M. U., Diniz C. R., Barbosa T. S. A comparison of the effects of scorpion venom tityustoxin and ouabain on the release of acetylcholine from incubated slices of rat brain. J. Neurochem. 1975; 24: 331
  • Henriques M. C., Gomez M. V. The effect of scorpion venom Tityustoxin on the uptake of calcium in synaptosomes. Brain Res. Bull. 1981; 7: 255
  • Freire Maia L., Cunha-Melo J. R., Gomez M. U., Tafuri W. L., Maria T. A., Calixto S. L., Futuro-Neto A. Studies on the mechanism of action of Tityustoxin, A. Ohsaka, K. Hayashi, S. Yoshio. Animal, Plant and microbial toxins Plenum Publishing, New York 1976; v.2: 273
  • Diniz C. R., Pimenta A. F., Coutinho-Netto J., Gomez M. V., Larson R. E. Tityustoxin as a neuropharmacological tool. Special publications Sarajevo 1976; 29(4)183, (Academy of Sciences and Arts of Bosnia and Herzegovia)
  • Vital Brazil O., Neder A. C., Corrado A. P. Effects and mechanism of action of Tityus serrulatus venom on skeletal muscle. Pharmacol. Res. Commun. 1973; 5: 137
  • Barhanin J., Giglio J. R., Leopold P., Schmid A., Sampaio S. V., Lazdunski M. Tityus serrulatus venom contains two classes of toxins. J. Biol. Chem. 1982; 257: 12553
  • Toledo D., Neves A. G.A. Purification and partial characterization of a second toxin from the scorpion Tityus serrulatus. Comp. Biochem. Physiol. 1976; 55B: 249
  • Bechis G., Sampieri F., Yuan P. M., Brando T., Martin M. F., Diniz C. R., Rochat H. Amino acid sequence of toxin VII, a β toxin from the venom of the scorpion Tityus serrulatus. Biochem. Biophys. Res. Comm. 1984; 122: 1146
  • Lombet A, Lazdunski M. Characterization, solubilization, affinity labeling and purification of the cardiac sodium channel using Tityus toxin gamma. Eur. J. Biochem. 1984; 141: 651
  • Pauron D., Barhanin J., Lazdunski M. The voltage-dependent sodium channel of insect nervous system identified by receptor sites for tetrodotoxin and scorpion and sea anemone toxins. Biochem. Biophys. Res. Commun. 1985; 131(1)1226
  • De Lima M. E., Martin M. F., Diniz C. R., Rochat H. Tityus serrulatus bears pharmacological properties of both β-toxin and insect toxin from scorpion venoms. Biochem. Biophys. Res. Comm. 1986; 139: 296
  • De Lima M. E., Couraud F., Hue B., Martin M. F., Lapied B., Pelhate M., Diniz C. R., Rochat H. “Insect toxins” from scorpion venoms as tools for the study of the sodium channel of insects. Pesticide Ssci. 1988; 24(3)284
  • De Lima M. E., Couraud F., Lapied B., Pelhate M., Diniz C. R., Rochat H. Photoaffinity labeling of scorpion toxin receptors associated with synaptosomal Na+ channels. Biochem Biophys. Res. Comm. 1988; 151: 187
  • De Lima M. E., Martin-Eauclaire M. F., Hue B., Loret E., Diniz C. R., Rochat H. On the binding of two scorpion toxins to the central nervous system of the cockroach Periplaneta americana. Insect Biochemistry 1989; 19: 413
  • De Lima M. E. Étude de l'interaction des toxines de scorpion avec le systeme nerveux des insects: Contribution à la caracterisation de leur rěcepteur. PhD. thesis, Marseille, France 1988; 147
  • Loret E., Sampieri F., Roussel A., Granier C., Rochat H. Conformational flexibility of a scorpion active on mammals and insects :a circular dichroism study. Proteins 1990; 8: 164
  • Barhanin J., Pauron D., Lombet A., Norman R. I., Vijverberg P. M., Giglio J. R., Lazdunski M. Electrophysiological characterization, solubilization and purification of the Tityus gamma toxin receptor associated with the gating of the sodium channel from rat brain. EMBO J. 1983; 2(6)915
  • Vijverberg H. P.M., Pauron O., Lazdunski M. The effects of Tityus serrulatus scorpion toxin γ on Na channels in neuroblastoma cells. Pfluges Arch. 1984; 401: 297
  • Zaborovskaya L. D., Khodorov B. I. Effect of Tityus γ toxin on the activation process in sodium channels of frog myelinated nerve. Gen. Physiol. Biophys 1985; 4: 101
  • Jonas P., Vogel W., Arantes E. C., Giglio J. R. Toxin gamma of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane. Pflugers Arch. 1986; 407(1)92
  • Oliveira M. J., Fontana M. O., Giglio J. R., Sampaio S. V., Corrado A. P., Prado W. A. Effects of the venom of the Brazilian scorpion Tityus serrulatus. and two of its fractions on the isolated diaphragm of the rat. Gen. Pharmacol. 1989; 20(2)202
  • Yatani A., Kirsch G. E., Possani L. D., Brown A. M. Effects of New World scorpion toxins on single-channel and whole cell cardiac sodium currents. Am. J. Physiol. 1988; 254: H443
  • Barhanin J., Schmid A., Lombet A., Wheeler K., Lazdunski M. Molecular size of different neurotoxin receptors on the voltage sensitive sodium channel. J. Biol. Chem. 1983; 258: 700
  • Darbon Jover H. E., Couraud F., Rochat H. Photoaffinity labeling of α and β-scorpion toxin receptors associated with rat brain sodium channel. Biochem. Biophys Res. Commun. 1983; 115: 415
  • Sharkey R. G., Beneski D. A., Catterall W. A. Differential labeling of the α and βt subunits of the sodium channel by photoreactive derivatives of scorpion toxins. Biochemistry 1984; 23: 6078
  • Seagar M. J., Labbé-Jullié C., Granier C., Goll A., Glossman H., Van Rietschoten J., Couraud F. Molecular structure of rat brain apamin receptor: differential photoaffinity labeling of putative K+ channel subunits and target size analysis. Biochemistry 1986; 25: 4051
  • Possani Martin L. D.B.M., Mochca-Morales J., Svendsen I. Purification and chemical characterization of the major toxins from the venom of the Brazilian scorpion Tityus serrulatus, Lutz and Mello. Carlsberg Res Comm. 1981; 46: 195
  • Possani L. D., Martin B. M., Fletcher M. D., Fletcher P. L. Discharge effect on pancreatic exocrine secretion produced by toxins purified from Tityus serrulatus scorpion venom. J. Biol. Chem. 1991; 266: 3178
  • Sampaio S. V., Laure C. J., Giglio J. R. (1983). Isolation a nd characterization of toxic proteins from the venom of the Brazilian scorpion Tityus serrulatus. Toxicon 1983; 21: 265
  • Arantes E. C., Prado Sampaio W. A.S.V., Giglio J. R. A simplified procedure for the fractionation of Tityus serrulatus venom: isolation and partial characterization of TsTX-IV, a new neurotoxin. Toxicon 1989; 27: 907
  • Possani L. D., Alagon A. C., Fletcher J. R.P.L., Erickson B. W. Purification and properties of mammalian toxins from the venom of the Brazilian scorpion Tityus serrulatus. Lutz and Mello. Arch. Biochem. Biophys 1977; 180: 394
  • Possani L. D., Martin B. M., Svendsen I., Rode Erickson G. S.B.W. Scorpion toxins from Centruroides noxius and Tityus serrulatus. Biochem. J. 1985; 229: 739
  • Martin-Eauclaire M. F., Céard B., Ribeiro A. M., Diniz C. R., Rochat H., Bougis P. E. Molecular cloning and nucleotide sequence analysis of a cDNA encoding the main β-neurotoxin from the venom of the South American scorpion Tityus serrulatus. FEBS Lett. 1992; 302: 220
  • Becerril B., Corona Mejia M., Martin M. C., Lucas B. M., Bolivar S. F., Possani L. D. The genomic region encoding toxin gamma from the scorpion Tityus serrulatus. contains anintron. FEBS Lett. 1993; 335: 6
  • Possani L. D., Martin B. M., Swendsen I. The primary structure of noxius toxin : a K+ channel blocking peptide, purified from the the venom of the scorpion Centruroides noxius Hoffman. Carlsberg. Res. Comm. 1982; 47: 285
  • Céard B., De Lima M. E., Bougis P. E., Martin-Eauclaire M. F. Purification of the main β-toxin from Tityus serrulatus venom using HPLC. Toxicon 1992; 30: 105
  • Mansuelle P., Martin-Eauclaire M. F., Chavez-Olortegui C., De Lima M. E., Rochat H., Granier C. The β-type toxin Ts II from the scorpion Tityus serrulatus : amino acid sequence determination and assessement of biological and antigenic properties. Natural toxins 1992; 1: 119
  • Darbon Jover H. E., Couraud F., Rochat H. α-scorpion neurotoxin derivatives suitable as potential markers of sodium channels. Preparation and characterization. Int. J. Peptide Protein Res. 1983; 22: 179
  • Bahraoui El E., Ayeb M., Van Rietschoten J., Rochat H., Granier C. Immunochemistry of scorpion α-toxins : study with synthetic peptides of the antigenicity of four regions of toxin II of Androctonus australis Hector. Mol. Immunol. 1986; 23: 357
  • Arantes E. C., Sampaio S. V., Vieira C. A., Giglio J. R. (1992) What is Tityustoxin ?. Toxicon 1992; 7: 786
  • De Lima M. E., Martin-Eauclaire M. F., Chavez-Olortegui C., Diniz C. R., Granier C. Tityus serrulatus venom toxins display a complex pattern of antigenic reactivity. Toxicon 1993; 31: 223
  • Marangoni S., Gliso J., Sampaio S. V., Arantes E. C., Giglio Oliveira J. R.B., Frangiose B. (1990). The complete amino acid sequence of toxin TsTX VI isolated from the venom of the scorpion Tityus serrulatus. J. Protein Chem. 1990; 9: 595
  • Possani L. D., Steinmetz W. E., Dent M. A.R., Alagon A. C., Wüthrich K. Preliminary spectroscopic characterization of six toxins from latin american scorpions. Biochem. Biophys. Acta. 1981; 669: 183
  • Arěas E. P.G., Giglio J. R., Arantes E. C., Kawano Y. Raman and infrared spectra of toxin γ from the venom of the scorpion Tityus serrulatus. Biochem. Biophys. Acta 1987; 915: 292
  • Delori R., Van Rietschoten J., Rochat H. Scorpion venoms and neurotoxins : an immunological study. Toxicon 1981; 19: 393
  • El Ayeb M., Delori P., Rochat H. Immunochemistry of scorpion toxins: antigenic homologies checked with radioimmunoassays. Toxicon 1983; 21: 709
  • Martin M. F., Garcia Y, Perez G., El Ayeb M., Kopeyan C., Bechis G., Jover E., Rochat H. Purification and chemical and biological characterization of seven toxins from the mexican scorpion, Centruroides suffusus suffusus. J. Biol. Chem. 1987; 262: 4452
  • Chavez-Olortegui C., Ait-Amara D., Rochat H., Diniz C., Granier C. In vivo protection against scorpion toxins by liposomal immunization. Vaccine 1991; 9: 907
  • Heneine L. G.D., Cordoso V. N., Daniel J. P., Heneine I. F. Detoxification of the T2 fraction from a scorpion (Tityus serrulatus., Lutz and Mello) venom by iodination and some immunogenic properties of the derivatives. Toxicon 1986; 24: 501
  • Nishikawa A. K., Caricati C. P., Lima M. L.S.R., Dos Santos M. C., Kiponis T. L., Eiskstedt V. R.D., Knysak I., Da Silva M. H., Higashi H. O., Dias da Silva W. Antigenic cross-reactivity among the venoms from several species of Brazilian scorpions. Toxicon 1994; 32: 989
  • Bougis P., Rochat H., Smith L. A. Precursors of Androctonus australis scorpion neurotoxin: structure of precursors, precessing outcomes and expression of a functional recombinant toxin II. J. Biol. Chem. 1989; 264: 19259
  • Zilberberg N., Zlotkin E., Gurevitz M. The cDNA sequences of a depressant insect selective neurotoxin from the scorpion Buthatus judaicus. Toxicon 1991; 29: 1155
  • Strong P. N. Potassium channel toxins. Pharmacol. J. 1990; 46: 137
  • Bontems F., Roumestand C., Gilquin B., Menez A., Toma F. Refined structure of charybdotoxin: common motifs in scorpion toxins and insect defensins. Science 1991; 254: 1521
  • Carbone E., Wanke E., Prestipino G., Possani L. D., Haelicke A. Selective blockage of voltage-dependent potassium channels by a novel scorpion toxin. Nature 1982; 296: 90
  • Blaustein M. P., Rogowski Schneider R. S.M.J., Krueger B. K. Polypeptide toxins from the venoms of Old World and New World scorpions preferentially block different potassium channels. Mol. Pharmacol. 1991; 40: 932
  • Werkman T. R., Gustafson T. A., Rogowski R. S., Blaustein M. P., Rogawski M. A. Tityus toxin-Kα, a structurally novel and highly potent K+ channel peptide toxin, interacts with the α-dendrotoxin binding site on the cloned Kv1–2 K+ channel. Mol. Pharmacol. 1993; 44: 430
  • Rogowski R. S., Krueger B. K., Collins J. H., Blaustein M. P. Tityus toxin-Kα blocks voltage-gated noninactivating K+ channels and unblocks inactivating K+ channels blocked by α-dendrotoxin in synaptosomes. Proc. Natl. Sci. USA 1994; 91: 1475

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