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ORIGINAL ARTICLE

PCR for enteric pathogens in high-prevalence settings. What does a positive signal tell us?

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Pages 491-498 | Received 29 Sep 2014, Accepted 18 Feb 2015, Published online: 12 Mar 2015

References

  • Ravdin JI. Diagnosis of invasive amoebiasis – time to end the morphology era. Gut 1994;35:1018–21.
  • Mirelman D, Nuchamowitz Y, Stolarsky T. Comparison of use of enzyme-linked immunosorbent assay-based kits and PCR amplification of rRNA genes for simultaneous detection of Entamoeba histolytica and E. dispar. J Clin Microbiol 1997;35:2405–7.
  • Blessmann J, Buss H, Nu PA, Dinh BT, Ngo QT, Van AL, et al. Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J Clin Microbiol 2002;40:4413–17.
  • Verweij JJ, Blangé RA, Templeton K, Schinkel J, Brienen EA, van Rooyen MA, et al. Simultaneous detection of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum in fecal samples by using multiplex real-time PCR. J Clin Microbiol 2004;42:1220–3.
  • Roy S, Kabir M, Mondal D, Ali IK, Petri WA Jr, Haque R. Real-time-PCR assay for diagnosis of Entamoeba histolytica infection. J Clin Microbiol 2005;43:2168–72.
  • Haque R, Roy S, Siddique A, Mondal U, Rahman SM, Mondal D, et al. Multiplex real-time PCR assay for detection of Entamoeba histolytica, Giardia intestinalis, and Cryptosporidium spp. Am J Trop Med Hyg 2007;76:713–17.
  • Bruijnesteijn van Coppenraet LE, Wallinga JA, Ruijs GJ, Bruins MJ, Verweij JJ. Parasitological diagnosis combining an internally controlled real-time PCR assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. Clin Microbiol Infect 2009;15:869–74.
  • ten Hove RJ, van Esbroeck M, Vervoort T, van den Ende J, van Lieshout L, Verweij JJ. Molecular diagnostics of intestinal parasites in returning travellers. Eur J Clin Microbiol Infect Dis 2009;28:1045–53.
  • Hamzah Z, Petmitr S, Mungthin M, Leelayoova S, Chavalitshewinkoon-Petmitr P. Development of multiplex real-time polymerase chain reaction for detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in clinical specimens. Am J Trop Med Hyg 2010;83:909–13.
  • Liang SY, Hsia KT, Chan YH, Fan CK, Jiang DD, Landt O, et al. Evaluation of a new single-tube multiprobe real-time PCR for diagnosis of Entamoeba histolytica and Entamoeba dispar. J Parasitol 2010;96:793–7.
  • Stark D, Al-Qassab SE, Barratt JL, Stanley K, Roberts T, Marriott D, et al. Evaluation of multiplex tandem real-time PCR for detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in clinical stool samples. J Clin Microbiol 2011;49:257–62.
  • Taniuchi M, Verweij JJ, Noor Z, Sobuz SU, Lieshout L, Petri WA Jr, et al. High throughput multiplex PCR and probe-based detection with Luminex beads for seven intestinal parasites. Am J Trop Med Hyg 2011;84:332–7.
  • Wiemer D, Loderstaedt U, von Wulffen H, Priesnitz S, Fischer M, Tannich E, et al. Real-time multiplex PCR for simultaneous detection of Campylobacter jejuni, Salmonella, Shigella, and Yersinia species in fecal samples. Int J Med Microbiol 2011;311:577–84.
  • Frickmann H, Schwarz NG, Wiemer DF, Fischer M, Tannich E, Scheid PL, et al. Food and drinking water hygiene and intestinal protozoa in deployed German soldiers. Eur J Microbiol Immunol (Bp) 2013;3:53–60.
  • Yavzori M, Uriel N, Porat N, Dagan R, Ambar R, Shpilberg O, et al. Development of molecular tests for rapid detection of enteropathogens. Harefuah 2000;138:758–62.
  • Evans AS. Causation and disease: the Henle-Koch postulates revisited. Yale J Biol Med 1976;49:175–95.
  • Carter KC. Koch's postulates in relation to the work of Jacob Henle and Edwin Klebs. Med Hist 1985;29:353–74.
  • Gaydos CA, Crotchfelt KA, Howell MR, Kralian S, Hauptman P, Quinn TC. Molecular amplification assay to detect chlamydial infections in urine specimens from high school female students and to monitor the persistence of chlamydial DNA after therapy. J Infect Dis 1998;177:417–24.
  • Rovery C, Greub G, Lepidi H, Casalta JP, Habib G, Collart F, et al. PCR detection of bacteria on cardiac valves of patients with treated bacterial endocarditis. J Clin Microbiol 2005;43:163–7.
  • van den Bijllaardt W, Overdevest IT, Buiting AG, Verweij JJ. Rapid clearance of Giardia lamblia DNA from the gut after successful treatment. Clin Microbiol Infect 2014;20:O972–4.
  • Kato K, Miura M. Comparative examinations. Jap J Parasitol 1954;3:35.
  • Katz N, Chaves A, Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 1972;14:397–400.
  • Schwarz NG, Rakotozandrindrainy R, Heriniaina JN, Randriamampionona N, Hahn A, Hogan B, et al. Schistosoma mansoni in schoolchildren in a Madagascan highland school assessed by PCR and sedimentation microscopy and Bayesian estimation of sensitivities and specificities. Acta Trop 2014;134:89–94.
  • Frickmann H, Dekker D, Boahen K, Acquah S, Sarpong N, Adu-Sarkodie Y, et al. Increased detection of invasive enteropathogenic bacteria in pre-incubated blood culture materials by real-time PCR in comparison with automated incubation in Sub-Saharan Africa. Scand J Infect Dis 2013; 45:616–22.
  • Verweij JJ, Laeijendecker D, Brienen EAT, van Lieshout L, Polderman AM. Detection of Cyclospora cayetanensis in travellers returning from the tropics and subtropics using microscopy and real-time PCR. Int J Med Microbiol 2003;293:199–202.
  • Basuni M, Muhi J, Othman N, Verweij JJ, Ahmad M, Miswan N, et al. A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths. Am J Trop Med Hyg 2011;84:338–43.
  • Niesters HGM. Quantitation of viral load using real-time amplification techniques. Methods 2001;25:419–29.
  • Frickmann H, Tenner-Racz K, Eggert P, Schwarz NG, Poppert S, Tannich E, et al. Influence of parasite density and sample storage time on the reliability of Entamoeba histolytica-specific PCR from formalin-fixed and paraffin-embedded tissues. Diagn Mol Pathol 2013;22:236–44.
  • Perneger TV. What's wrong with Bonferroni adjustments. BMJ 1998;316:1236–8.
  • Verweij JJ. Application of PCR-based methods for diagnosis of intestinal parasitic infections in the clinical laboratory. Parasitology 2014;29:1–10.
  • Basuni M, Mohamed Z, Ahmad M, Zakaria NZ, Noordin R. Detection of selected intestinal helminths and protozoa at Hospital Universiti Sains Malaysia using multiplex real-time PCR. Trop Biomed 2012;29:434–42.
  • Verweij JJ, Pit DS, van Lieshout L, Baeta SM, Derry GD, Gasser RB, et al. Determining the prevalence of Oesophagostomum bifurcum and Necator americanus infections using specific PCR amplification of DNA from fecal samples. Trop Med Int Health 2001;9:726–31.
  • Koehler KM, Lasky T, Fein SB, Delong SM, Hawkins MA, Rabatsky-Ehr T, et al. Population-based incidence of infection with selected bacterial enteric pathogens in children younger than five years of age, 1996–1998. Pediatr Infect Dis J 2006;25:129–34.
  • Moss DM, Priest JW, Hamlin K, Derado G, Herbein J, Petri WA Jr, et al. Longitudinal evaluation of enteric protozoa in Haitian children by stool exam and multiplex serologic assay. Am J Trop Med Hyg 2014;90:653–60.
  • Mejia R, Vicuña Y, Broncano N, Sandoval C, Vaca M, Chico M, et al. A novel, multi-parallel, real-time polymerase chain reaction approach for eight gastrointestinal parasites provides improved diagnostic capabilities to resource-limited at-risk populations. Am J Trop Med Hyg 2013;88:1041–7.
  • Liu J, Kabir F, Manneh J, Lertsethtakarn P, Begum S, Gratz J, et al. Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. Lancet Infect Dis 2014;14:716–24.
  • Platts-Mills JA, Gratz J, Mduma E, Svensen E, Amour C, Liu J, et al. Association between stool enteropathogen quantity and disease in Tanzanian children using TaqMan array cards: a nested case-control study. Am J Trop Med Hyg 2014;90:133–8.
  • Lindsay B, Ochieng JB, Ikumapayi UN, Toure A, Ahmed D, Li S, et al. Quantitative PCR for detection of Shigella improves ascertainment of Shigella burden in children with moderate-to-severe diarrhea in low-income countries. J Clin Microbiol 2013;51:1740–6.

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