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Original Article

Anti-SS-A/Ro antibody determination by indirect immunofluorescence and comparison of different methods of anti-nuclear antibody screening

Evaluation of the utility of HEp-2 cells transfected with the 60 kDa SS-A/Ro as a substrate

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Pages 585-592 | Received 21 Mar 2008, Accepted 03 Jun 2008, Published online: 02 Jan 2014

References

  • Tan EM. Autoantibodies to nuclear antigens (ANA): immuno-biology and medicine. Adv Immunol 1982;33:167–240.
  • Muro Y. Antinuclear antibodies. Autoimmunity 2005;38:3–9.
  • Wolin SL, Steitz JA. The Ro small cytoplasmic ribonucleopro-teins: identification of the antigenic protein and its binding site on the Ro RNAs. Proc Natl Acad Sci U S A 1984;81:1996–2000.
  • Ben-chetrit E, Chan EKL, Sullivan KF, Tan EM. A 52-1d3 protein is a novel component of the SS-A/Ro antigenic particle. J Exp Med 1988;167: 1560–71.
  • Gaither KK, Fox OF, Yamagata H, Mamula MJ, Reichlin M, Harley JB. Implications of anti-Ro/Sjögren's syndrome A antigen autoantibody in normal sera for autoimmunity. J Clin Invest 1987;79:841–6.
  • Harmon CE, Deng JS, Peebles CL, Tan EM. The importance of tissue substrate in the SS-A/Ro antigen-antibody system. Arthritis Rheum 1984;27:166–73.
  • Xia P, Fritz KA, Geoghegan WD, Jordon RE. The particulate (speckled-like thread) nuclear staining pattern: species and cel-lular distribution of the Ro/SS-A antigen. J Clin Lab Immunol 1987;22:101–5.
  • Clark G, Reichlin M, Tomasi TB. Characterization of a soluble cytoplasmic antigen reactive with sera from patients with sys-temic lupus erythematosus. J Immunol 1969;102:117–22.
  • Takano S, Matsushima H, Hiwatashi T, Miyachi K. Detection of anti-SS-A/Ro antibody by using pig spleen supernatant and characterization of corresponding antigen. Jpn J Rheumatol 1989;2:67–78.
  • Forman MS, Nakamura M, Mimori T, Gelpi C, Hardin JA. Detection of antibodies to small nuclear ribonucleoproteins and small cytoplasmic ribonucleoproteins using unlabeled cell extracts. Arthritis Rheum 1985;28:1356–61.
  • Rader MD, O'Brien C, Liu Y, Harley JB, Reichlin M. Hetero-geneity of the Ro/SSA antigen: different molecular forms in lymphocytes and red blood cells. J Clin Invest 1989;83:1293–8.
  • Buyon JP, Slade SG, Chan EKL, Tan EM, Winchester R. Effective separation of the 52 kDa SSA/Ro polypeptide from the 48 kDa SSB/La polypeptide by altering conditions of polyacryl-amide gel electrophoresis. J Immunol Methods 1990;129: 207–10.
  • Slobbe RL, Pruijn GJM, Damen WGM, van der Kemp JWCM, van Venrooij WJ. Detection and occurrence of the 60-and 52-1d3 Ro-(SSA) antigens and of autoantibodies against these proteins. Clin Exp Immunol 1991;86: 99–105.
  • Manoussakis MN, Kistis KG, Liu X, Aidinis V, Guialis A, Moutsopoulos HM. Detection of anti-Ro(SS-A) antibodies in autoimmune diseases: comparison of five methods. Br J Rheu-matol 1993;32:449–55.
  • Meilof JF, Bantjes I, de Jong J, van Dam AP, Smeenk RJT. The detection of anti-Ro(SS-A) and anti-La(SS-B) antibodies a comparison of counterimmunoelectrophoresis with immunoblot, ELISA, and RNAprecipitation assays. J Immunol Methods 1990;133:215–26.
  • Bossuyt X, Meurs L, Mewis A, Marien G, Blanckaert N. Screening for autoantibodies to SS-A/Ro by indirect immuno-fluorescence using HEp-2000TM cells. Ann Clin Biochem 2000;37: 216–9.
  • von Mühlen CA, Tan EM. Autoantibodies in the diagnosis of systemic rheumatic diseases. Semin Arthritis Rheum 1995;24: 323–58.
  • Hendrick JP, Wolin SL, Rinke J, Lemer MR, Steitz JA. Ro small cytoplasmic ribonucleoproteins are a subclass of La ribonucleo-proteins: further characterization of the Ro and La small ribonucleoproteins from uninfected mammalian cells. Mol Cell Biol 1981;1:1138–48.
  • Keech CL, McCluskey J, Gordon TP. Transfection and overex-pression of the human 60-kDa Ro/SS-A autoantigen in HEp-2 cells. Clin Immunol Immunopathol 1994;73: 146–51.
  • Fritzler MJ, Miller BJ. Detection of autoantibodies to SS-A/Ro by indirect immunofluorescence using a transfected and overex-pressed human 60 kD Ro autoantigen in HEp-2 cells. J Clin Lab Anal 1995;9:218–24.
  • Keech CL, Howarth S, Coates T, Rischmueller M, McCluskey J, Gordon TP. Rapid and sensitive detection of anti-Ro (SS-A) antibodies by indirect immunofluorescence of 60 kDa Ro HEp-2 transfectants. Pathology 1996;28:54–7.
  • Pollock W, Toh BH. Routine immunofluorescence detection of Ro/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro/SS-A. J Clin Pathol 1999;52:684–7.
  • Morozzi G, Bellisai F, Simpatico A, Pucci G, Bacarelli MR, Campanella V, et al. Comparison of different methods for the detection of anti-Ro/SSA antibodies in connective tissue diseases. Clin Exp Rheumatol 2000;18:729–31.
  • Peene I, Van Ael W, Vandenbossche M, Vervaet T, Veys E, De Keyser F. Sensitivity of the HEp-2000 substrate for the detection of anti-SSA/Ro60 antibodies. Clin Rheumatol 2000;19:291–5.
  • Boey ML, Peebles CL, Tsay G, Feng PH, Tan EM. Clinical and autoantibody correlations in Orientals with systemic lupus ery-thematosus. Ann Rheum Dis 1988;47:918–23.
  • Miyagawa S. Neonatal lupus erythematosus: a review of the racial differences and similarities in clinical, serological and immunogenetic features of Japanese versus Caucasian patients. J Dermatol 2005;32:514–22.
  • Watanabe A, Kodera M, Sugiura K, Usuda T, Tan EM, Takasaki Y, et al. Anti-DFS70 antibodies in 597 healthy hospital workers. Arthritis Rheum 2004;50:892–900.
  • Vitali C, Bombardieri S, Moutsopoulos HM, Balestrieri G, Bencivelli W, Bernstein RM, et al. Preliminary criteria for the classification of Sjögren's syndrome: results of a prospective concerted action supported by the European Community. Arthritis Rheum 1993;36:340–7.
  • Tan EM, Cohen AS, Fries JF, Masi AT, McShane DJ, Rothfield NF, et al. The 1982 revised criteria for the classification of sys-temic lupus erythematosus. Arthritis Rheum 1982;25:1271–7.
  • No authors listed. Preliminary criteria for the classification of systemic sclerosis (scleroderma). Subcommittee for Scleroderma Criteria of the American Rheumatism Association Diagnostic and Therapeutic Criteria Committee. Arthritis Rheum 1980;23:581–90.
  • Bohan A, Peter JB. Polymyositis and dermatomyositis. N Engl J Med 1975;292:344–7.
  • Bossuyt X, Frans J, Hendrickx A, Godefridis G, Westhovens R, Mariön G. Detection of anti-SSA antibodies by indirect immu-nofluorescence. Clin Chem 2004;50:2361–9.
  • Miranda-Cartis ME, Boutjdir M, Tseng CE, DiDonato F, Chan EKL, Buyon JP. Induction of antibodies reactive with SSA/Ro-SSB/La and development of congenital heart block in a murine model. J Immunol 1998;161:5886–92.
  • Fouraux MA, Bouvet P, Verkaart S, van Venrooij WJ, Pruijn GJ. Nucleolin associates with a subset of the human Ro ribonucleo-protein complexes. J Mol Biol 2002;320:475–88.
  • Fritzler MJ, Hanson C, Miller J, Eystathioy T. Specificity of autoantibodies to SS-A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate. J Clin Lab Anal 2002;16:103–8.

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