Advanced search
Publication Cover
Infection Ecology & Epidemiology Volume 6, 2016 - Issue 1
Submit an article Journal homepage
699
Views
11
CrossRef citations to date
0
Altmetric
Original Research Articles

Detection of Echinococcus multilocularis by MC-PCR: evaluation of diagnostic sensitivity and specificity without gold standard

Helene WahlströmDepartment of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, SwedenCorrespondence[email protected]
, DVM, PhD, Dipl. ECVPH,
Arianna CominDepartment of Disease Control and Epidemiology, National Veterinary Institute, Uppsala, Sweden
, DVM, PhD,
Mats IsakssonDepartment of Microbiology, National Veterinary Institute, Uppsala, Sweden
&
Peter DeplazesInstitute of Parasitology, Vetsuisse and Medical Faculty, University of Zurich, Zurich, Switzerland
, Prof. Dr. med. vet., EVPC
Article: 30173 | Received 26 Oct 2015, Accepted 28 Jan 2016, Published online: 09 Mar 2016

References

  • Vuitton DA, Demonmerot F, Knapp J, Richou C, Grenouillet F, Chauchet A, etal. Clinical epidemiology of human AE in Europe. Vet Parasitol. 2015; 213: 110–20. [PubMed Abstract].
  • Craig PS, Rogan MT, Allan JC. Detection, screening and community epidemiology of taeniid cestode zoonoses: cystic echinococcosis, alveolar echinococcosis and neurocysticercosis. Adv Parasitol. 1996; 38: 169–250. [PubMed Abstract].
  • Conraths FJ, Deplazes P. Echinococcus multilocularis: epidemiology, surveillance and state-of-the-art diagnostics from a veterinary public health perspective. Vet Parasitol. 2015; 213: 149–61. [PubMed Abstract].
  • Raoul F, Hegglin D, Giraudoux P. Trophic ecology, behaviour and host population dynamics in Echinococcus multilocularis transmission. Vet Parasitol. 2015; 213: 162–71. [PubMed Abstract].
  • Gottstein B, Stojkovic M, Vuitton DA, Millon L, Marcinkute A, Deplazes P. Threat of alveolar echinococcosis to public health – a challenge for Europe. Trends Parasitol. 2015; 31: 407–12. [PubMed Abstract].
  • Isaksson M, Hagstrom A, Armua-Fernandez M, Wahlstrom H, Agren E, Miller A, etal. A semi-automated magnetic capture probe based DNA extraction and real-time PCR method applied in the Swedish surveillance of Echinococcus multilocularis in red fox (Vulpes vulpes) faecal samples. Parasite Vector. 2014; 7: 583.
  • Øines Ø, Isaksson M, Hagstrom A, Tavornpanich S, Davidson RK. Laboratory assessment of sensitive molecular tools for detection of low levels of Echinococcus multilocularis-eggs in fox (Vulpes vulpes) faeces. Parasite Vector. 2014; 7: 246.
  • Thrushfield M. Veterinary epidemiology. 2005; Oxford, UK: Blackwell. 3rd ed 330, 567.
  • Deplazes P, Alther P, Tanner I, Thompson RC, Eckert J. Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations. J Parasitol. 1999; 85: 115–21. [PubMed Abstract].
  • Karamon J, Sroka J, Cencek T. Limit of detection of sedimentation and counting technique (SCT) for Echinococcus multilocularis diagnosis, estimated under experimental conditions. Exp Parasitol. 2010; 124: 244–6. [PubMed Abstract].
  • Rindskopf D. The use of latent class analysis in medical diagnosis. Annual Meeting of the American Statistical Association. 2002. New York, 11–15 August 2002, pp. 2912–16..
  • Hofer S, Gloor S, Muller U, Mathis A, Hegglin D, Deplazes P. High prevalence of Echinococcus multilocularis in urban red foxes (Vulpes vulpes) and voles (Arvicola terrestris) in the city of Zurich, Switzerland. Parasitology. 2000; 120: 135–42. [PubMed Abstract].
  • Branscum AJ, Gardner IA, Johnson WO. Estimation of diagnostic-test sensitivity and specificity through Bayesian modeling. Prev Vet Med. 2005; 68: 145–63. [PubMed Abstract].
  • Spiegelhalter DJ, Best NG, Carlin BP, Van Der Linde A. Bayesian measures of model complexity and fit. J R Statist Soc B. 2002; 64: 583–639.
  • Eckert J, Gemmell MA, Meslin FX, Pawlowski ZS. WHO/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. 2001; 265. Available from: http://whqlibdoc.who.int/publications/2001/929044522X.pdf [cited 20 August 2015].
  • Hegglin D, Bontadina F, Contesse P, Gloor S, Deplazes P. Plasticity of predation behaviour as a putative driving forse for parasite life-cycle dynamics: the case of urban foxes and Echinococcosis multilocularis tapeworm. Funct Ecol. 2007; 21: 552–60.
  • Burlet P, Deplazes P, Hegglin D. Age, season and spatio-temporal factors affecting the prevalence of Echinococcus multilocularis and Taenia taeniaeformis in Arvicola terrestris . Parasite Vector. 2011; 4: 6.
  • Stieger C, Hegglin D, Schwarzenbach G, Mathis A, Deplazes P. Spatial and temporal aspects of urban transmission of Echinococcus multilocularis . Parasitology. 2002; 124: 631–40. [PubMed Abstract].
  • Veit P, Bilger B, Schad V, Schafer J, Frank W, Lucius R. Influence of environmental factors on the infectivity of Echinococcus multilocularis eggs. Parasitology. 1995; 110: 79–86. [PubMed Abstract].
  • Dinkel A, von Nickisch-Rosenegk M, Bilger B, Merli M, Lucius R, Romig T. Detection of Echinococcus multilocularis in the definitive host: coprodiagnosis by PCR as an alternative to necropsy. J Clin Microbiol. 1998; 36: 1871–6. [PubMed Abstract] [PubMed CentralFull Text].
  • Al-Sabi MN, Kapel CM, Deplazes P, Mathis A. Comparative copro-diagnosis of Echinococcus multilocularis in experimentally infected foxes. Parasitol Res. 2007; 101: 731–6. [PubMed Abstract].
  • Toft N, Jorgensen E, Hojsgaard S. Diagnosing diagnostic tests: evaluating the assumptions underlying the estimation of sensitivity and specificity in the absence of a gold standard. Prev Vet Med. 2005; 68: 19–33. [PubMed Abstract].

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.