Advanced search
Publication Cover
Journal of Extracellular Vesicles Volume 4, 2015 - Issue 1
Journal homepage
52,877
Views
1,034
CrossRef citations to date
0
Altmetric
Technical Reports

Optimized exosome isolation protocol for cell culture supernatant and human plasma

Richard J. LobbTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia;School of Medicine University of Queensland, Brisbane, QLD, Australia
,
Melanie BeckerTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
,
Shu Wen WenTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
,
Christina S. F. WongTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
,
Adrian P. WiegmansTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia
,
Antoine LeimgruberDepartment of Medical Imaging, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland;Service of Nuclear Medicine and Molecular Imaging Department of Medical Imaging, University Hospital of Lausanne, Switzerland
&
Andreas MöllerTumour Microenvironment Laboratory QIMR Berghofer Medical Research Institute, Herston, QLD, Australia;School of Medicine University of Queensland, Brisbane, QLD, AustraliaCorrespondence[email protected]
 show all
Article: 27031 | Received 22 Dec 2014, Accepted 06 Aug 2015, Published online: 17 Jul 2015

References

  • Vlassov AV, Magdaleno S, Setterquist R, Conrad R. Exosomes: current knowledge of their composition, biological functions, and diagnostic and therapeutic potentials. Biochim Biophys Acta. 2012; 1820: 940–8.
  • Wendler F, Bota-Rabassedas N, Franch-Marro X. Cancer becomes wasteful: emerging roles of exosomes(dagger) in cell-fate determination. J Extracell Vesicles. 2013; 2: 22390. http://dx.doi.org/10.3402/jev.v2i0.22390.
  • EL Andaloussi S, Mager I, Breakefield XO, Wood MJ. Extracellular vesicles: biology and emerging therapeutic opportunities. Nat Rev Drug Discov. 2013; 12: 347–57.
  • Thery C, Ostrowski M, Segura E. Membrane vesicles as conveyors of immune responses. Nat Rev Immunol. 2009; 9: 581–93.
  • Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006. Chapter 3:Unit 3.22.
  • Van Deun J, Mestdagh P, Sormunen R, Cocquyt V, Vermaelen K, Vandesompele Jet al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles. 2014; 3: 24858. http://dx.doi.org/10.3402/jev.v3.24858.
  • Lamparski HG, Metha-Damani A, Yao JY, Patel S, Hsu DH, Ruegg C et al. Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Methods. 2002; 270: 211–26.
  • Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013; 200: 373–83.
  • Mathivanan S, Fahner CJ, Reid GE, Simpson RJ. ExoCarta 2012: database of exosomal proteins, RNA and lipids. Nucleic Acids Res. 2012; 40(Database issue): 1241–4.
  • Simpson RJ, Kalra H, Mathivanan S. ExoCarta as a resource for exosomal research. J Extracell Vesicles. 2012; 1: 18374. http://dx.doi.org/10.3402/jev.v1i0.18374.
  • Thery C, Boussac M, Veron P, Ricciardi-Castagnoli P, Raposo G, Garin J et al. Proteomic analysis of dendritic cell-derived exosomes: a secreted subcellular compartment distinct from apoptotic vesicles. J Immunol. 2001; 166: 7309–18.
  • Webber J, Clayton A. How pure are your vesicles?. J Extracell Vesicles. 2013; 2: 19861. http://dx.doi.org/10.3402/jev.v2i0.19861.
  • Kalra H, Adda CG, Liem M, Ang CS, Mechler A, Simpson RJ et al. Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma. Proteomics. 2013; 13: 3354–64.
  • Simpson RJ, Lim JW, Moritz RL, Mathivanan S. Exosomes: proteomic insights and diagnostic potential. Expert Rev Proteomics. 2009; 6: 267–83.
  • Caby MP, Lankar D, Vincendeau-Scherrer C, Raposo G, Bonnerot C. Exosomal-like vesicles are present in human blood plasma. Int Immunol. 2005; 17: 879–87.
  • Witwer KW, Buzas EI, Bemis LT, Bora A, Lasser C, Lotvall Jet al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles. 2013; 2: 20360. http://dx.doi.org/10.3402/jev.v2i0.20360.
  • Lai RC, Chen TS, Lim SK. Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease. Regen Med. 2011; 6: 481–92.
  • Gould SJ, Raposo G. As we wait: coping with an imperfect nomenclature for extracellular vesicles. J Extracell Vesicles. 2013; 2: 20389. http://dx.doi.org/10.3402/jev.v2i0.20389.
  • Cvjetkovic A, Lotvall J, Lasser C. The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles. J Extracell Vesicles. 2014; 3: 23111. http://dx.doi.org/10.3402/jev.v3.23111.
  • Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM et al. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods. 56; 2012: 293–304.
  • Bobrie A, Colombo M, Krumeich S, Raposo G, Thery C. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 2012; 1: 18397. http://dx.doi.org/10.3402/jev.v1i0.18397.
  • Momen-Heravi F, Balaj L, Alian S, Trachtenberg AJ, Hochberg FH, Skog J et al. Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles. Front Physiol. 2012; 3: 162.
  • Boing AN, van der Pol E, Grootemaat AE, Coumans FA, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 2014; 3: 23430. http://dx.doi.org/10.3402/jev.v3.23430.
  • Lotvall J, Hill AF, Hochberg F, Buzas EI, Di Vizio D, Gardiner Cet al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. J Extracell Vesicles. 2014; 3: 26913. http://dx.doi.org/10.3402/jev.v3.26913.
  • Jeppesen DK, Hvam ML, Primdahl-Bengtson B, Boysen AT, Whitehead B, Dyrskjot Let al. Comparative analysis of discrete exosome fractions obtained by differential centrifugation. J Extracell Vesicles. 2014; 3: 25011. http://dx.doi.org/10.3402/jev.v3.25011.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.