1,923
Views
43
CrossRef citations to date
0
Altmetric
Original Articles

Genotoxic effect induced by hydrogen peroxide in human hepatoma cells using comet assay

, , &
Article: 4637 | Received 04 Sep 2009, Published online: 13 Jan 2010

References

  • Dong-Hyun L, Timothy R, Gred P. Oxidative DNA damage induced by copper and hydrogen peroxide promotes CG–TT tandem mutations at methylated CpG dinucleotides in nucleotide excision repair-deficient cells. Nucleic Acids Res. 2002; 30: 3566–73.
  • Cadet JD, Douki T, Gasparutto T, Pouget D, Ravanat JP, Sauvaigo JL. Hydroxyl radicals and DNA base damage. Mutat Res. 1999; 424: 9–21.
  • Kleiman NJ, Wang RR, Spector A. Hydrogen peroxide-induced DNA damage in bovine lens epithelial cells. Mutat Res. 1990; 240: 35–45.
  • Subhashinee SK, Wijeratne LC, Vikcy S. Hydrogen peroxide induced oxidative stress damage and antioxidant enzyme response in Caco-2 human colon cells. J Agric Food Chem. 2005; 53: 8768–74.
  • Davies K. Oxidative stress, antioxidant defenses and damage removal, repair and replacement systems. IUBMB Life. 2000; 50: 279–89.
  • Jornot PH, Junod A. Hydrogen peroxide-induced DNA damage is independent of nuclear calcium but dependent on redox-active ions. Biochem J. 1998; 335: 85–94.
  • Sun-Yee K, Ryung-Hyo K, Tae-Lin H, Jeen-Woo P. α-Phenyl-N-t-butylnitrone protects oxidative damage to HepG2 cells. J Biochem Mol Biol. 2000; 34: 43–6.
  • Piperakis M, Karanastasi G, Lakovidou-Kritsi Z, Piperakis M. The use of comet assay in measuring DNA damage and repair efficiency in child, adult, and old age populations. Cell Biol Toxicol. 2009; 25: 65–71.
  • Collins A. Oxidative DNA damage, antioxidants, and cancer. Bioessays. 1999; 21: 238–46.
  • Tenopoulou M, Doulias P, Barbouti A, Brunk U, Galaris D. Role of compartmentalized redox-active iron hydrogen peroxide-induced DNA damage and apoptosis. Biochem J. 2005; 387: 703–10.
  • Rojas E, Lopez M, Valverde M. Single cell gel cell electrophoresis assay: methodology and applications. J Chromatogr B Biomed Sci Appl. 1999; 722: 225–54.
  • Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al.. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35: 206–21.
  • Singh NP, Tice DB, Pearson RR, Brant JD, Schneider EL. DNA damage and repair with age in individual human lymphocytes. Mutat Res. 1990; 237: 123–30.
  • Philips HJ. Dye exclusion tests for cell viability. In: Kruse Jr. PF, Patterson Jr. MKDye exclusion tests for cell viability. NY: Academic press. 1973. p. 406–8.
  • Wang CC, Fang KM, Yang CS, Tzeng SF. Reactive oxygen species-induced cell death of rat primary astrocytes through mitochondria-mediated mechanism. J Cell Biochem. 2009; 107: 933–43.
  • Wang K, Hong YJ, Huang ZQ. Protective effects of silybin on human umbilical vein endothelial cell injury induced by H2O2 in vitro. Vascul Pharmacol. 2005; 43: 198–206.
  • Frenzilli GB, Barale E. Validation of single cell gel assay in human leukpcytes with 18 reference compounds. Mutat Res. 2000; 468: 93–108.
  • Rosignoli P, Fabiani R, De Bartolomeo A, Spinozzi F, Agea E, Pelli MA, et al.. Protective activity of butyrate on hydrogen peroxide-induced DNA damage in isolated human colonocytes and HT29 tumor cells. Carcinogenesis. 2001; 22: 1675–80.
  • Gasiorowski K, Brokos B. DNA repair of hydrogen peroxide-induced damage in human lymphocytes in the presence of four antimutagens. A study with alkaline single cell gel electrophoresis (Comet assay). Cell Mol Biol Lett. 2001; 6: 897–911.
  • Henzler T, Steudle E. Transport and metabolic degradation of hydrogen peroxide in chara corallina: model calculations and measurements with the pressure probe suggest transport of H2O2 across water channels. J Exp Bot. 2000; 51: 2053–66.
  • Jaruga P, Dizdaroglu M. Repair of products of oxidative DNA base damage in human cells. Nucleic Acids Res. 1996; 24: 1389–94.