Cladonia pyxidata and C. pocillum; genetic evidence to regard them as conspecific

LITERATURE CITED

• Ahti T. 1966. Correlation of the chemical and morphological characters in Cladonia chlorophaea and allied lichens. Ann Bot Fenn 3:380–390.
   Google Scholar
• ———. 2000. Cladoniaceae. Flora Neotropica Monograph 78. New York: New York Botanical Garden. 362 p.
   Google Scholar
• Aptroot A, Sipman HJM, van Herk CM. 2001. Cladonia monomorpha, a neglected cup lichen from Europe. Lichenologist 33:271–283.
   Google Scholar
• Beiggi S, Piercey-Normore MD. 2007. Evolution of ITS ribosomal RNA secondary structures in fungal and algal symbionts of selected species of Cladonia sect. Cladonia (Cladoniaceae, Ascomycotina). J Mol Evol 64:528–542.
   Google Scholar
• Bergamini A, Stofer S, Bolliger J, Scheidegger C. 2007. Evaluating macrolichens and environmental variables as predictors of the diversity of epiphytic microlichens. Lichenologist 39:475–489.
   Google Scholar
• Bingle LEH, Simpson TJ, Lazarus CM. 1999. Ketosynthase domain probes identify two subclasses of fungal polyketide synthase genes. Fungal Genet Biol 26:209–223.
   Google Scholar
• Culberson CF. 1972. Improved conditions and new data for the identification of lichen products by a standardized thin-layer chromatographic method. J Chromatogr 72: 113–125.
   Google Scholar
• ———, Armaleo D. 1992. Induction of a complete secondary-product pathway in a cultured lichen fungus. Exp Mycol 16:52–63.
   Google Scholar
• ———, Culberson WL, Johnson A. 1988. Gene flow in lichens. Am J Bot 75:1135–1139.
   Google Scholar
• DePriest PT. 1993. Variation in the Cladonia chlorophaea complex I: morphological and chemical variation in southern Appalachian populations. Bryologist 96:555–563.
   Google Scholar
• ———. 1994. Variation in the Cladonia chlorophaea complex II: ribosomal DNA variation in a southern Appalachian population. Bryologist 97:117–126.
   Google Scholar
• Felsenstein J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791.
   Google Scholar
• Gilbert OL. 1977. Fungal plasticity in Cladonia pocillum. Lichenologist 9:172–173.
   Google Scholar
• Green TGA, Lange OL. 1991. Ecophysiological adaptations of the lichen genera Pseudocyphellaria and Sticta to south temperate rainforests. Lichenologist 23:267–282.
   Google Scholar
• Grube M, DePriest PT, Gargas A, Hafellner J. 1995. DNA isolation from lichen ascomata. Mycol Res 99:1321–1324.
   Google Scholar
• Hawksworth DL. 1976. Ecological factors and species delimitation in the lichens. In: Heywood VH, ed. Taxonomy and ecology. New York: Academic Press. p 31–69.
   Google Scholar
• Helms G, Friedl T, Rambold G, Mayrhofer H. 2001. Identification of photobionts from the lichen family Physciaceae using algal-specific ITS rDNA sequencing. Lichenologist 33:73–86.
   Google Scholar
• Huelsenbeck JP, Ronquist F, Nielsen R, Bollback JP. 2001. Bayesian inference of phylogeny and its impact on evolutionary biology. Science 294:2310–2314.
   Google Scholar
• Jones JB Jr. 2001. Laboratory guide for conducting soil tests and plant analysis. New York: CRC Press. 256 p.
   Google Scholar
• Leuckert C, Ahmadjian V, Culberson CF, Johnson A. 1990. Xanthones and depsidones of the lichen Lecanora dispersa in nature and of its mycobiont in culture. Mycologia 82:370–378.
   Google Scholar
• Miadlikowska J, Lutzoni F. 2000. Phylogenetic revision of the genus Peltigera (lichen-forming ascomycetes) based on morphological, chemical and large subunit nuclear ribosomal DNA data. Int J Plant Sci 161:925–958.
   Google Scholar
• O’Brien HE, Miadlikowska J, Lutzoni F. 2009. Assessing reproductive isolation in highly diverse communities of the lichen-forming fungal genus Peltigera. Evolution 63: 2076–2086.
   Google Scholar
• Ohmura Y, Kawachi M, Kasai F, Watanabe MM. 2006. Genetic combinations of symbionts in a vegetatively reproducing lichen, Parmotrema tinctorum, based on ITS rDNA sequences. Bryologist 109:43–59.
   Google Scholar
• Osyczka P. 2006. The lichen genus Cladonia (Cladoniaceae, lichenized Ascomycota) from Spitsbergen. Pol Polar Res 27:207–242.
   Google Scholar
• Page RDM. 1996. TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358.
   Google Scholar
• Piercey-Normore MD. 2004. Selection of algal partners by lichen fungi and patterns of variation for three species of Cladonia. Can J Bot 82:947–961.
   Google Scholar
• ———. 2006. The lichen-forming ascomycete Evernia mesomorpha associates with multiple genotypes of Trebouxia jamesii. New Phytol 169:331–344.
   Google Scholar
• ———, DePriest PT. 2001. Algal switching among lichen symbioses. Am J Bot 88:1490–1498.
   Google Scholar
• Posada D, Coppins BJ, Hawksworth DL, James PW, Moore DM. 1992. The lichen flora of Great Britain and Ireland. London: Natural History MuseumPUBLICations & British Lichen Society. 710 p.
   Google Scholar
• ———, Crandall KA. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14:817–818.
   Google Scholar
• Rambaut A. 2001. Se-Al (sequence alignment editor). Version 1. Oxford, UK: Department Zoology, Univ Oxford.
   Google Scholar
• Richardson DHS. 1991. Lichens as biological indicators—recent developments. In: Jeffrey DW, Madden B, eds. Bioindicators and environmental management. London, San Diego: Academic Press. p 263–272.
   Google Scholar
• ———. 1992. Pollution monitoring with lichens. Naturalists’ handbooks 19, Slough. England: Richmond Publishing Co. Ltd. 76 p.
   Google Scholar
• Rikkinen J. 1997. Habitat shifts and morphological variation of Pseudevernia furfuracea along a topographical gradient. Symb Bot Ups 32:223–245.
   Google Scholar
• Romeike J, Friedl T, Helms G, Ott S. 2002. Genetic diversity of algal and fungal partners in four species of Umbilicaria (lichenized ascomycetes) along a transect of the Antarctic peninsula. Mol Biol Evol 19:1209–1217.
   Google Scholar
• Ronquist F, Huelsenbeck JP. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574.
   Google Scholar
• Skaloud P, Peksa O. 2007. Phylogeny, morphology and taxonomic revision of the symbiotic alga Asterochloris (Trebouxiophyceae, Chlorophyta). 4th European phycological Congress, 23–27 Jul 2007. Oviedo, Spain. Poster P1Mi-16. 108 p.
   Google Scholar
• Stenroos S, Hyvonen J, Myllys L, Thell A, Ahti T. 2002. Phylogeny of the genus Cladonia s. lat. (Cladoniaceae, Ascomycetes) inferred from molecular, morphological and chemical data. Cladistics 18:237–278.
   Google Scholar
• Stocker-Wörgötter E. 2001. Experimental lichenology and microbiology of lichens: culture experiments, secondary chemistry of cultured mycobionts, resynthesis and thallus morphogenesis. Bryologist 104:576–581.
   Google Scholar
• Swofford DL. 2003. PAUP*: phylogenetic analysis using parsimony (*and other methods). Version 4. Sunderland, Massachusetts: Sinauer Associates.
   Google Scholar
• Thomson JW. 1967. The lichen genus Cladonia in North America. Toronto, Canada: Univ Toronto Press. 172 p.
   Google Scholar
• ———. 1984. American Arctic Lichens 1. The Macrolichens. New York: Columbia Univ Press. 504 p.
   Google Scholar
• White TJ, Bruns TD, Lee SB, Taylor JW. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR protocols: a guide to methods and applications. New York: Academic Press. p 315–322.
   Google Scholar
• Yahr R, Vilgalys R, DePriest PT. 2004. Strong fungal specificity and selectivity for algal symbionts in Florida scrub Cladonia lichens. Mol Ecol 13:3367–3378.
   Google Scholar