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Mycologia Volume 106, 2014 - Issue 2
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Original Articles

Use of the vital stain FUN-1 indicates viability of Phytophthora capsici propagules and can be used to predict maximum zoospore production

Melanie L. Lewis Ivey
&
Sally A. Miller Ohio State University-Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691Correspondence[email protected]
Pages 362-367 | Received 07 Jan 2013, Accepted 05 Aug 2013, Published online: 20 Jan 2017

Literature cited

  • BanuettFHerskowitzI. 2002. Bud morphogenesis and the actin and microtubule cytoskeletons during budding in the corn smut fungus, Ustilago maydis. Fungal Genet Biol 37:149–170, 10.1016/S1087-1845(02)00548-0
  • ButtTMHochHCStaplesRCSt LegerRJ. 1989. Use of fluorochromes in the study of fungal cytology and differentiation. Exp Mycol 13:303–320, 10.1016/0147-5975(89)90026-1
  • ChenCYSeguin-SwartzG. 2002. A rapid method for assessing the viability of fungal spores. Can J Plant Pathol 24:230–232, 10.1080/07060660309507000
  • El-HamalawiZAErwinDC. 1986. Physical, enzymatic and chemical factors affecting viability and germination of oospores of Phytophthora megasperma f. sp. medicaginis. Phytopathology 76:503–507, 10.1094/Phyto-76-503
  • ErwinDCRiberiroOK. 1996. Phytophthora Diseases Worldwide. St Paul, Minnesota: American Phytopathological Society Press.
  • EtxeberriaAMendarteSLarreglaS. 2011. Determination of viability of Phytophthora capsici oospores with the tetrazolium bromide staining test versus a plasmolysis method. Rev Iberoam Micol 28:43–49, 10.1016/j.riam.2010.11.005
  • HansenEMReeserPWSuttonW. 2012. Phytophthora beyond agriculture. Annu Rev Phytopathol 50:359–378, 10.1146/annurev-phyto-081211-172946
  • Henry-StanleyMJGarniRMWellsCL. 2004. Adaption of FUN-1 and calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells. J Microbiol Methods 59:289–292, 10.1016/j.mimet.2004.07.001
  • HochHCGalvaniCDSzarowskiDHTurnerJN. 2005. Two new fluorescent dyes applicable for visualization of fungal cell walls. Mycologia 97:580–588, 10.3852/mycologia.97.3.580
  • HughesJMcCullyME. 1975. The use of an optical brightener in the study of plant structure. Stain Technol 50:319–329.
  • KimSAhnIPRhoHSLeeYH. 2005. MHPI, a Magnaporthe grisea hydrophobin gene, is required for fungal development of plant colonization. Mol Microbiol 57:1224–1237, 10.1111/j.1365-2958.2005.04750.x
  • Lass-FlörlCNaglMSpethCUlmerHDierichMPWürznerR. 2001. Studies of in vitro activities of voriconazole and itraconazole against Aspergillus hyphae using viability staining. Antimicrob Agents Chemother 45:124–128, 10.1128/AAC.45.1.124-128.2001
  • LatorseMPJarryFMercierVVelosoS. 2007. Tuber blight control: effect of foliar applied fungicides such as Infinito on viability of sporangia of Phytophthora infestans. In: SchepersHTAM, ed. Proceedings of the 10th workshop of an European network for development of an integrated control strategy for potato late blight. Wageningen: Applied Plant Research Wageningen University and Research Centre. p 37–47.
  • LeveneH. 1960. Robust tests for equality of variances. In: OlkinI, ed. Contributions to probability and statistics. Palo Alto: Stanford Univ. Press. p 278–292.
  • Lewis IveyML. 2011. Assessing microbial risks and management strategies in vegetables [doctoral dissertation]. Columbus: Ohio State Univ. Press. 476 p.
  • MillardPJRothBLThiHPYueSTHauglandRP. 1997. Development of the FUN-1 family of fluorescent probes for vacuole labeling and viability testing of yeasts. Appl Environ Microbiol 63:2897–2905.
  • OardSRushMCOardJH. 2004. Characterization of antimicrobial peptides against a US strain of the rice pathogen Rhizoctonia solani. J Appl Microbiol 97:169–180, 10.1111/j.1365-2672.2004.02291.x
  • RiedelMWagnerSGotzMBelbarhiLLefortFWerresS. 2007. Studies of tissue colonization in rhododendron by Phytophthora ramorum. In: FrankelSJKliejunasJTPalmieriKM, eds. Proceedings of the sudden oak death 3rd science symposium. Albany, New York: U.S. Forest Service. p 485–487.
  • RonceroCJonesEW. 1985. Fluorescence microscopy methods for yeasts. Methods Cell Biol 31:357–435.
  • SergeevaVNairNLegendreLDarleyESpooner-HartR. 2002. The use of flurochromes to determine the effect of chlorine dioxide on survival of Plasmopara viticola on grapevine. Aust Plant Pathol 31:295–297, 10.1071/AP02018
  • SutherlandEDCohenSD. 1983. Evaluation of tetrazolium bromide as a vital stain for fungal oospores. Phytopathology 73:1532–1535, 10.1094/Phyto-73-1532
  • VittalRHaudenshieldJSHartmanGL. 2012. A multiplexed immunofluorescence method identifies Phakopsora pachyrhizi urediniospores and determines their viability. Phytopathology 102:1143–1152, 10.1094/PHYTO-02-12-0040-R

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