The Use of Quantitative Real Time Polymerase Chain Reaction to Quantify Some Rumen Bacterial Strains in an In Vitro Rumen System

References

• AOAC, 2000. Official Methods of Analysis, 17th ed., Association of Official Analytical Chemists, Gaithersburg, MD, USA.
   Google Scholar
• CardozoP.W. CalsamigliaS. FerretA. KamelC. 2005. Screening for the effects of natural plant extracts at two pH level on in vitro rumen microbial fermentation of a high-concentrate diet for beef cattle. J. Anim. Sci. 83:2572-2579.
   PubMed Web of Science ®Google Scholar
• ChurchD.C. 1988. The ruminant animal: digestive physiology and nutrition. Prentice-Hall Ed., Englewood Cliffs, NJ, USA.
   Google Scholar
• DehorityB.A. OrpinC.G. 1988. Development of, and natural fluctuations in, rumen microbial populations. In: HobsonP.N. ( ed.) The rumen microbial ecosystem. Elsevier Publ., London, UK, pp 151-183.
   Google Scholar
• GetachewG. CrovettoG.M. FondevilaM. KrishnamoorthyU. SinghB. SpangheroM. SteingassH. RobinsonP.H. 2002. Laboratory variation of in vitro gas production and estimated metabolizable energy values of ruminant feeds. Anim. Feed Sci. Tech. 102:169-180.
   Google Scholar
• GudlaP. AbuGhazalehA.A. IshlakA. JonesK. 2011. The effect of level of forage and oil supplement on biohydrogenation intermediates and bacteria in continuous cultures. Anim. Feed. Sci. Tech. 171:108-116.
   Google Scholar
• HenningP.H. HornC.H. LeeuwK.J. MeissnerH.H. HaggF.M. 2010a. Effect of ruminal administration of the lactate-utilizing strain Megasphaera elsdenii (Me) NCIMB 41125 on abrupt or gradual transition from forage to concentrate diets. Anim. Feed Sci. Tech. 157:20-29.
   Web of Science ®Google Scholar
• HenningP.H. HornC.H. SteynD.G. MeissnerH.H. HaggF.M. 2010b. The potential of Megasphaera elsdenii isolates to control ruminal acidosis. Anim. Feed Sci. Tech. 157:13-19.
   Google Scholar
• HuwsS.A. LeeM.R. MuetzelS.M. ScottM.B. WallaceR.J. ScollanN.D. 2010. Forage type and fish oil cause shifts in rumen bacterial diversity. FEMS Microbiol. Ecol. 73:396-407.
   PubMed Web of Science ®Google Scholar
• KlieveA.V. HennesseyD. OuwerkerkD. ForsterR.J. MackieR.I. AttwoodG.T. 2003. Establishing populations of Megasphaera elsdenii YE 34 and Butyrivibrio fibrisolvens YE 44 in the rumen of cattle fed high-grain diets. J. Appl. Microbiol. 95:621-630.
   PubMed Web of Science ®Google Scholar
• KoikeS. KobayashiY. 2001. Development and use of competitive PCR assays for the rumen cellulolytic bacteria: Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FEMS Microbiol. Lett. 204:361-366.
   PubMed Web of Science ®Google Scholar
• KoikeS. KobayashiY. 2009. Fibrolytic rumen bacteria: their ecology and functions. Asian Austral. J. Anim. 22:131-138.
   Web of Science ®Google Scholar
• LathamM.J. StorryJ.E. SharpeM.E. 1972. Effect of low-roughage diets on the microflora and lipid metabolism in the rumen. J. Appl. Microbiol. 24:871-877.
   Google Scholar
• MartínezM.E. RanillaM.J. TejidoM.L. RamosS. CarroM.D. 2010. Comparison of fermentation of diets of variable composition and microbial populations in the rumen of sheep and Rusitec fermenters. I. Digestibility, fermentation parameters, and microbial growth. J. Dairy Sci. 93: 3684-3698.
   Google Scholar
• Menke K.H. Steingass 1988. Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Anim. Res. Dev. 28:7-55.
   Google Scholar
• MuetzelS. LawrenceP. HoffmannE. M. BeckerK. 2009. Evaluation of a stratified continuous rumen incubation system. Anim. Feed. Sci. Tech. 151: 32-43.
   Google Scholar
• PalmonariA. StevensonD.M. MertensD.R. CruywagenC.W. WeimerP.J. 2010. pH dynamics and bacterial community composition in the rumen of lactating dairy cows. J. Dairy Sci. 93:279-287.
   PubMed Web of Science ®Google Scholar
• PopovaM. MartinC. EugèneM. MialonM.M. DoreauM. MorgaviD.P. 2011. Effect of fibre and starch-rich finishing diets on methanogenic Archaea diversity and activity in the rumen of feedlot bulls. Anim. Feed. Sci. Tech. 166-167:113-121.
   Web of Science ®Google Scholar
• SpangheroM. BerzaghiP. FortinaR. MasoeroF. RapettiL. ZanfiC. TassoneS. GalloA. 2010. Technical note: precision and accuracy of in vitro digestion of neutral detergent fiber and predicted net energy of lactation content of fibrous feeds. J. Dairy Sci. 93:4855-4859.
   PubMed Web of Science ®Google Scholar
• SpeightS.M. HarmonD.L. 2010. Batch culture evaluation of carbohydrate inhibitors to moderate rumen fermentation. Anim. Feed. Sci. Tech. 155:156-162.
   Web of Science ®Google Scholar
• StevensonD.M. WeimerP.J. 2007. Dominance of Prevotella and low abundance of classical ruminal bacterial species in the bovine rumen revealed by relative quantification real-time PCR. Appl. Microbiol. Biotechnol. 75:165-174.
   PubMed Web of Science ®Google Scholar
• TajimaK. AminovR.I. NagamineT. MatsuiH. NakamuraM. BennoY. 2001. Dietdependent shifts in the bacterial population of the rumen revealed with Real-Time PCR. Appl. Environ. Microb. 67:2766-2774.
   PubMed Web of Science ®Google Scholar
• TilleyJ.M.A. TerryR.A. 1963. A two-stage method for the in vitro digestion of forage crops. J. Brit. Grassland Soc. 18:104-111.
   Google Scholar
• Van SoestP.J. RobertsonJ.B. LewisB.A. 1991. Method for dietary fibre, neutral detergent fibre and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
   PubMed Web of Science ®Google Scholar
• WeimerP.J. StevensonD.M. MertensD.R. HallM.B. 2011. Fiber digestion, VFA production, and microbial population changes during in vitro ruminal fermentations of mixed rations by monensin-adapted and unadapted microbes. Anim. Feed. Sci. Tech. 160:68-78.
   Google Scholar